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Membranes
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Analytical Sciences Of / With Bio(mimetic) Membranes
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Analytical Sciences Of / With Bio(mimetic) Membranes

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Membrane Analysis and Characterization".

Deadline for manuscript submissions: closed (20 June 2022) | Viewed by 13131

Special Issue Editor

Dr. Yukihiro Okamoto

E-Mail Website
Guest Editor
Division of Chemical Engineering, Graduated School of Engineering Science, Osaka University, Osaka 565-0871, Japan
Interests: analysis of lipid bilayer; separation with lipid membrane; interface, micro–nano separation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Analytical Sciences of/with cell and bio(mimetic) membranes have been very important in fundamental and applied research. For example, analysis of cell membranes can reveal the mechanism of cell membrane function. In addition, artificial bio-membranes can attain superior performance as sensors and imaging tools. Thus, novel and high-performance analytical sciences of/with cell and bio(mimetics) have been demanded for the progress of biology and for practical use.

This Special Issue on “Analytical Sciences Of / With Bio(mimetic) Membranes” of the journal Membranes seeks manuscripts on bio(mimetic) membrane analysis and analysis with bio(mimetic) membranes. Topics include but are not limited to the analysis of cell membranes or biomimetic membranes such as liposomes, sensors or separation with bio(mimetic) membranes, in fundamental or applied research. Authors are invited to submit their latest results in original papers, and reviews are also welcome.

Dr. Yukihiro Okamoto
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cell membrane
  • biomimetic membrane
  • analytical science
  • sensor
  • separation

Published Papers (5 papers)

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Research

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16 pages, 4696 KiB  
Article
Interaction of Lipophilic Cytarabine Derivatives with Biomembrane Model at the Air/Water Interface
by Jhon Fernando Berrio Escobar, Cristiano Giordani, Stefano Russo, Francesco Castelli and Maria Grazia Sarpietro
Membranes 2022, 12(10), 937; https://doi.org/10.3390/membranes12100937 - 27 Sep 2022
Cited by 1 | Viewed by 1250
Abstract
Cell membrane models are useful for obtaining molecular-level information on the interaction of biologically active molecules whose activity is believed to depend also on their effects on the membrane. Cytarabine was conjugated with fatty acids to improve the drug lipophilicity and the interaction [...] Read more.
Cell membrane models are useful for obtaining molecular-level information on the interaction of biologically active molecules whose activity is believed to depend also on their effects on the membrane. Cytarabine was conjugated with fatty acids to improve the drug lipophilicity and the interaction with the biomembrane model. Cytarabine was conjugated with fatty acids of different lengths to form the trimyristoyl cytarabine and the tristearoyl cytarabine derivatives. Their interaction with biomembrane models constituted by dimyristoylphosphatidylcholine (DMPC) monolayers was studied by employing the Langmuir–Blodgett technique. DMPC/cytarabine, DMPC/trimyristoyl cytarabine and DMPC/tristearoyl cytarabine mixed monolayers at increasing molar fractions of the compound were prepared and placed on the subphase. The mean molecular area/surface pressure isotherms were recorded at 37 °C. Between the molecules of DMPC and those of cytarabine or prodrugs, repulsive forces act. However, these forces are very weak between DMPC and cytarabine and stronger between DMPC and the cytarabine derivatives, thus avoiding the expulsion of the compounds at higher surface pressure and modifying the stability of the mixed monolayer. The fatty acid moieties could then modulate the affinity of cytarabine for biomembranes. Full article
(This article belongs to the Special Issue Analytical Sciences Of / With Bio(mimetic) Membranes)
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11 pages, 2536 KiB  
Article
Characterization of Phase Separated Planar Lipid Bilayer Membrane by Fluorescence Ratio Imaging and Scanning Probe Microscope
by Yukihiro Okamoto, Kaito Hamaguchi, Mayo Watanabe, Nozomi Watanabe and Hiroshi Umakoshi
Membranes 2022, 12(8), 770; https://doi.org/10.3390/membranes12080770 - 09 Aug 2022
Cited by 1 | Viewed by 1951
Abstract
The lipid membrane forms nanodomains (rafts) and shows heterogeneous properties. These nanodomains relate to significant roles in various cell functions, and thus the analysis of the nanodomains in phase-separated lipid membranes is important to clarify the function and role of the nanodomains. However, [...] Read more.
The lipid membrane forms nanodomains (rafts) and shows heterogeneous properties. These nanodomains relate to significant roles in various cell functions, and thus the analysis of the nanodomains in phase-separated lipid membranes is important to clarify the function and role of the nanodomains. However, the lipid membrane possesses small-sized nanodomains and shows a small height difference between the nanodomains and their surroundings at certain lipid compositions. In addition, nanodomain analysis sometimes requires highly sensitive and expensive apparatus, such as a two-photon microscope. These have prevented the analysis by the conventional fluorescence microscope and by the topography of the scanning probe microscope (SPM), even though these are promising methods in macroscale and microscale analysis, respectively. Therefore, this study aimed to overcome these problems in nanodomain analysis. We successfully demonstrated that solvatochromic dye, LipiORDER, could analyze the phase state of the lipid membrane at the macroscale with low magnification lenses. Furthermore, we could prove that the phase mode of SPM was effective in the visualization of specific nanodomains by properties difference as well as topographic images of SPM. Hence, this combination method successfully gave much information on the phase state at the micro/macro scale, and thus this would be applied to the analysis of heterogeneous lipid membranes. Full article
(This article belongs to the Special Issue Analytical Sciences Of / With Bio(mimetic) Membranes)
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15 pages, 2737 KiB  
Article
A Langmuir-Blodgett Study of the Interaction between Amphotericin B and Lipids of Histoplasma capsulatum
by Pedronel Araque-Marín, Andrea Naranjo Díaz, Luisa Fernanda Gómez Londoño, María del Pilar Jiménez Alzate, Francesco Castelli, Maria Grazia Sarpietro, Cristiano Giordani and Carlos Alberto Peláez Jaramillo
Membranes 2022, 12(5), 483; https://doi.org/10.3390/membranes12050483 - 29 Apr 2022
Viewed by 1750
Abstract
Histoplasma capsulatum is a dimorphic, thermal, and nutritional fungus. In the environment and at an average temperature of 28 °C, it develops as a mold that is composed of infecting particles. Once in the host or in cultures at 37 °C, it undergoes [...] Read more.
Histoplasma capsulatum is a dimorphic, thermal, and nutritional fungus. In the environment and at an average temperature of 28 °C, it develops as a mold that is composed of infecting particles. Once in the host or in cultures at 37 °C, it undergoes a transition into the parasitic form. In the present work, we performed chemical extraction and characterization using chromatography techniques of the associated lipid composition of the external surface of the cell wall of the mycelial phase of two isolates of the H. capsulatum: one clinical and one environmental. Several differences were evidenced in the fatty acids in the phospholipid composition. Surface pressure–area isotherms and compression module curves of the Amphotericin B and lipid extract monolayers, as well as (AmB)-lipid extract mixed monolayers were recorded. Results show a high affinity of AmB towards lipid extracts. The most stable monolayers were formed by AmB + environmental with a mass ratio of 1:3 and AmB + clinical with a mass ratio of 1:2. Knowledge of the AmB aggregation processes at a molecular level and the characterization of the lipid extracts allows the possibility to understand the interaction between the AmB and the lipid fractions of H. capsulatum. Full article
(This article belongs to the Special Issue Analytical Sciences Of / With Bio(mimetic) Membranes)
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19 pages, 4229 KiB  
Communication
Detection of Prostate Cancer via IR Spectroscopic Analysis of Urinary Extracellular Vesicles: A Pilot Study
by Xin-Le Yap, Bayden Wood, Teng-Aik Ong, Jasmine Lim, Bey-Hing Goh and Wai-Leng Lee
Membranes 2021, 11(8), 591; https://doi.org/10.3390/membranes11080591 - 31 Jul 2021
Cited by 10 | Viewed by 3045
Abstract
Extracellular vesicles (EVs) are membranous nanoparticles naturally released from living cells which can be found in all types of body fluids. Recent studies found that cancer cells secreted EVs containing the unique set of biomolecules, which give rise to a distinctive absorbance spectrum [...] Read more.
Extracellular vesicles (EVs) are membranous nanoparticles naturally released from living cells which can be found in all types of body fluids. Recent studies found that cancer cells secreted EVs containing the unique set of biomolecules, which give rise to a distinctive absorbance spectrum representing its cancer type. In this study, we aimed to detect the medium EVs (200–300 nm) from the urine of prostate cancer patients using Fourier transform infrared (FTIR) spectroscopy and determine their association with cancer progression. EVs extracted from 53 urine samples from patients suspected of prostate cancer were analyzed and their FTIR spectra were preprocessed for analysis. Characterization of morphology, particle size and marker proteins confirmed that EVs were successfully isolated from urine samples. Principal component analysis (PCA) of the EV’s spectra showed the model could discriminate prostate cancer with a sensitivity of 59% and a specificity of 81%. The area under curve (AUC) of FTIR PCA model for prostate cancer detection in the cases with 4–20 ng/mL PSA was 0.7, while the AUC for PSA alone was 0.437, suggesting the analysis of urinary EVs described in this study may offer a novel strategy for the development of a noninvasive additional test for prostate cancer screening. Full article
(This article belongs to the Special Issue Analytical Sciences Of / With Bio(mimetic) Membranes)
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Review

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20 pages, 4935 KiB  
Review
Structures and Dynamics of Native-State Transmembrane Protein Targets and Bound Lipids
by Michael Overduin, Catharine Trieber, R. Scott Prosser, Louis-Philippe Picard and Joey G. Sheff
Membranes 2021, 11(6), 451; https://doi.org/10.3390/membranes11060451 - 17 Jun 2021
Cited by 13 | Viewed by 4283
Abstract
Membrane proteins work within asymmetric bilayers of lipid molecules that are critical for their biological structures, dynamics and interactions. These properties are lost when detergents dislodge lipids, ligands and subunits, but are maintained in native nanodiscs formed using styrene maleic acid (SMA) and [...] Read more.
Membrane proteins work within asymmetric bilayers of lipid molecules that are critical for their biological structures, dynamics and interactions. These properties are lost when detergents dislodge lipids, ligands and subunits, but are maintained in native nanodiscs formed using styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers. These amphipathic polymers allow extraction of multicomponent complexes of post-translationally modified membrane-bound proteins directly from organ homogenates or membranes from diverse types of cells and organelles. Here, we review the structures and mechanisms of transmembrane targets and their interactions with lipids including phosphoinositides (PIs), as resolved using nanodisc systems and methods including cryo-electron microscopy (cryo-EM) and X-ray diffraction (XRD). We focus on therapeutic targets including several G protein-coupled receptors (GPCRs), as well as ion channels and transporters that are driving the development of next-generation native nanodiscs. The design of new synthetic polymers and complementary biophysical tools bodes well for the future of drug discovery and structural biology of native membrane:protein assemblies (memteins). Full article
(This article belongs to the Special Issue Analytical Sciences Of / With Bio(mimetic) Membranes)
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