Fluorescence Techniques for Exploring Protein Oligomerization
A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".
Deadline for manuscript submissions: 30 August 2024 | Viewed by 60
Special Issue Editor
Interests: biophysics; biophotonics; optics; optical spectroscopy; dielectric spectroscopy; fluorescence; Förster Resonance Energy Transfer (FRET); protein complex structure; imaging; dynamics
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Fluorescence from endogenous and exogenous chromophores is widely used for quantifying protein interactions with like molecules (called homo-oligomerization) or other receptors (hetero-oligomerization). For example, protein co-localization, either at the single molecule level or using super-resolution microscopy, detects protein–protein interactions by tracking the motion and physical proximity of two or more proteins within a cell. Methods based on Förster Resonance Energy Transfer (FRET) probe molecular association measure relative distances between molecules within an oligomer via the transfer of energy via coupling between the transition dipoles of optically excited and unexcited fluorescent tags attached to different molecules of interest. A third class of methods generically known as Fluorescence Fluctuation Spectroscopy probe fluctuations in fluorescence intensities from pixel to pixel in an image (i.e., spatial fluctuations) or from time to time (i.e., temporal fluctuations) to determine whether the molecules that produced the fluctuations diffuse around as monomers, dimers, or higher order oligomers. This Special Issue welcomes contributions from researchers interested in the quantitative studies of protein–protein or protein–ligand interactions in solution, fixed or living cells, and tissues as well as related problems of interest to biophysicists, biologists, biochemists, pharmacologists, and other life scientists.
Prof. Dr. Valerica Raicu
Guest Editor
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Keywords
- protein oligomerization
- Förster Resonance Energy Transfer (FRET)
- quantifying protein interactions
- single molecule fluorescence
- super-resolution microscopy
- fluorescence fluctuation spectroscopy
