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Horticulturae
Special Issues
Genetic Improvement and Breeding of Grape
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Genetic Improvement and Breeding of Grape

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Genetics, Genomics, Breeding, and Biotechnology (G2B2)".

Deadline for manuscript submissions: closed (20 February 2024) | Viewed by 3097

Special Issue Editors

Dr. Guotian Liu

E-Mail Website
Guest Editor
College of Horticulture, Northwest A&F University, Yangling 712100, China
Interests: grape; disease resistant; effector; breeding
Dr. Zhi Li

E-Mail Website
Guest Editor
College of Horticulture, Northwest A&F University, Yangling 712100, China
Interests: grape; fungal disease; effector; biocontrol
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Grape is widely grown because of its high fruit quality and adaptability in a wide range of climatic conditions. However, there are many limiting factors, such as fungal diseases, insect pests, drought, cold, changeable climate, etc. The objectives of grape genetic improvement must be to solve these emerging problems in addition to maintaining yield and quality. The aim of a number of grapevine breeding programs throughout the world is to develop new varieties or germplasms with higher quality and resistance to biotic and abiotic stresses as well as better adaptability. The purpose of this Special Issue on “Genetic Improvement and Breeding of Grape” is to discuss, among others, the following aspects: (1) gene identification and mapping of excellent grape traits, such as fragrant genes, seedless genes, and resistant genes; (2) development and assisted breeding of grape molecular markers; (3) breeding and identification of new grape varieties, such as improvement of grape embryo rescue breeding efficiency and identification of seedless lines; (4) application of grape transgenic technology; etc. We cordially invite researchers to contribute original research articles and reviews for this Special Issue and share your achievements in grape genetic improvement and breeding.

Dr. Guotian Liu
Dr. Zhi Li
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • grape breeding
  • new variety
  • molecular marker
  • biotic stress
  • abiotic stress
  • gene function
  • embryo rescue
  • transgenic technology

Published Papers (2 papers)

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Research

11 pages, 4262 KiB  
Article
Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes
by Yannan Chu, Min Li, Ruonan Li, Kangzhuang Zhang, Pengpeng Qiu, Xiaojian Yuan, Yulei Han, Xinyu Liu, Yan Xu and Guotian Liu
Horticulturae 2023, 9(9), 992; https://doi.org/10.3390/horticulturae9090992 - 02 Sep 2023
Cited by 1 | Viewed by 1184
Abstract
Seedlessness in grapes is much appreciated by consumers and especially in cultivars consumed either as table grapes or as raisins. In many parts of the world, low temperature is the main environmental stress limiting grape production. In this study, stenospermocarpic (seed abortion) cultivars [...] Read more.
Seedlessness in grapes is much appreciated by consumers and especially in cultivars consumed either as table grapes or as raisins. In many parts of the world, low temperature is the main environmental stress limiting grape production. In this study, stenospermocarpic (seed abortion) cultivars were selected as the female parents while seeded cold-resistant cultivars were selected as the male parents to develop new cold-resistant seedless grapes using embryo rescue technology, which has previously been shown to be a highly efficient way of breeding seedless grapes. Here, we report optima in genotype, sampling time, and culture medium for the embryo rescue of 14 hybrid combinations. Our results indicate that the embryo development rate (39.9%) and the seedlings rate (21.5%) were highest among the 14 crosses when ‘Ruby Seedless’ was used as the female parent and ‘Beibinghong’ was used as the male parent. The best sampling times for ‘Yuehong Wuhe’, ‘Ruby Seedless’, and ‘Melissa seedless’ were 37, 55, and 52 days after flowering, respectively. Embryo rescue efficiency was highest when the sucrose concentration for seedlings was maintained at about 1.0%. Using molecular markers, we detected 91 hybrids with seedless traits and 18 hybrids with cold resistance traits. Full article
(This article belongs to the Special Issue Genetic Improvement and Breeding of Grape)
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18 pages, 3849 KiB  
Article
Development, Identification and Validation of a Novel SSR Molecular Marker for Heat Resistance of Grapes Based on miRNA
by Lipeng Zhang, Yue Song, Junpeng Li, Jingjing Liu, Zhen Zhang, Yuanyuan Xu, Dongying Fan, Mingying Liu, Yi Ren, Xiaojun Xi, Qiuju Chen, Juan He, Wenping Xu, Shiren Song, Huaifeng Liu and Chao Ma
Horticulturae 2023, 9(8), 931; https://doi.org/10.3390/horticulturae9080931 - 16 Aug 2023
Cited by 2 | Viewed by 1090
Abstract
The adverse effects of high temperatures on fruit quality and yield have been reported in many studies of grapevines. MiRNAs play crucial roles in plant growth and development and also fulfill functions in regulating the high-temperature response. In this research, miRNA-based SSR (simple [...] Read more.
The adverse effects of high temperatures on fruit quality and yield have been reported in many studies of grapevines. MiRNAs play crucial roles in plant growth and development and also fulfill functions in regulating the high-temperature response. In this research, miRNA-based SSR (simple sequence repeat) polymorphisms were analyzed according to the grape genome sequence and then combined with polymerase chain reaction (PCR) amplification and Sanger sequencing methods to analyze the miRNA-SSR diversity of different heat-resistant grape varieties. A statistical analysis of SSR sequences showed that 391 and 12 SSRs were specific to pri-miRNA and pre-miRNA, respectively. In pri-miRNA containing SSR, hexa-nucleotide repeats were the most abundant (52.69%), followed by tetra-nucleotide (13.04%) and minimum penta-nucleotide (4.09%), which were also observed in pre-miRNA sequences. On the other hand, differences in heat resistance among grape varieties were observed. Based on the results of leaf Fv/Fm images, values and phenotypic changes under high-temperature stress, 20 heat-resistant (e.g., Niagara Rosada and Grand Noir) and 20 heat-sensitive (e.g., Shine Muscat and Jumeigui) grape varieties were identified. Further, PCR-Sanger sequencing was used to screen SSR differences in four thermos-tolerant and four thermos-sensitive grape varieties, and finally, eight SSR differential primers were found to be able to distinguish these varieties. Of these, four pairs of primers were selected for validation in 40 grape germplasm resources (20 thermo-tolerance and 20 thermo-sensitivity). The VMIRSSR167c3 highlights that the ratio of SSR (CT) repeat values greater than 17 in heat-resistant varieties was 90%. In conclusion, the VMIRSSR167c3 marker can accurately distinguish between heat-resistant and heat-sensitive grape varieties. The results provide a novel molecular marker for the genetic improvement of grape germplasm resources and will be beneficial to the breeding of heat-resistant varieties in the future. Full article
(This article belongs to the Special Issue Genetic Improvement and Breeding of Grape)
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