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Biology
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Exploring and Designing Novel Microbes for Biotechnology
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Exploring and Designing Novel Microbes for Biotechnology

A special issue of Biology (ISSN 2079-7737).

Deadline for manuscript submissions: closed (16 April 2021) | Viewed by 11914

Special Issue Editors

Dr. Michele Fabris

E-Mail Website
Guest Editor
CSIRO Synthetic Biology Future Science Platform, University of Technology Sydney, P.O. Box 123, Broadway, NSW 2007, Australia
Interests: algal and plant metabolism, algal and plant terpenoid metabolism; algae and plant molecular biology and genetics; algal strain engineering; algal biotechnology; synthetic biology; metabolic engineering
Dr. Raffaela Abbriano

E-Mail Website
Guest Editor
Climate Change Cluster, University of Technology Sydney, Broadway Campus, Ultimo NSW 2007, Sydney, Australia
Interests: phytoplankton evolution and metabolism; microalgal genomics; microbial strain engineering; algal biotechnology; synthetic biology

Special Issue Information

Dear Colleagues,

The transition from fossil-based production to more sustainable, bio-based manufacturing practices greatly depends on the capacity of organisms to provide a broad spectrum of products at large scale. Microbial-based bioproduction is a long sought goal, as it would offer low-cost cultivation requirements, specific metabolic advantages, and sustainability. The yet unexplored vast diversity of microbes contains features and traits that could offer novel biological solutions to industrial needs. Advancements in molecular biology, genetic engineering and sequencing technologies are increasingly aimed at new, non-model organisms, which are expected to fill these gaps.

The aim of this Special Issue is the investigation of novel natural and engineered traits that could have industrial potential in non-model organisms, including genomics, genetic engineering and synthetic biology efforts.

This issue will provide a timely collection on new fundamental and applied knowledge that is expected to foster and advance the industrial exploitation of microorganisms for bioproduction.

Dr. Michele Fabris
Dr. Raffaela Abbriano
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Novel microbial traits
  • Genomics
  • Functional genetics
  • Enzyme characterisation
  • Genome sequencing
  • Metabolism
  • Synthetic biology
  • Metabolic engineering
  • Bioproduction

Published Papers (2 papers)

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Research

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15 pages, 2316 KiB  
Article
Cloning of Thalassiosira pseudonana’s Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli
by Ryan R. Cochrane, Stephanie L. Brumwell, Arina Shrestha, Daniel J. Giguere, Samir Hamadache, Gregory B. Gloor, David R. Edgell and Bogumil J. Karas
Biology 2020, 9(11), 358; https://doi.org/10.3390/biology9110358 - 26 Oct 2020
Cited by 3 | Viewed by 3976
Abstract
Algae are attractive organisms for biotechnology applications such as the production of biofuels, medicines, and other high-value compounds due to their genetic diversity, varied physical characteristics, and metabolic processes. As new species are being domesticated, rapid nuclear and organelle genome engineering methods need [...] Read more.
Algae are attractive organisms for biotechnology applications such as the production of biofuels, medicines, and other high-value compounds due to their genetic diversity, varied physical characteristics, and metabolic processes. As new species are being domesticated, rapid nuclear and organelle genome engineering methods need to be developed or optimized. To that end, we have previously demonstrated that the mitochondrial genome of microalgae Phaeodactylum tricornutum can be cloned and engineered in Saccharomyces cerevisiae and Escherichia coli. Here, we show that the same approach can be used to clone mitochondrial genomes of another microalga, Thalassiosira pseudonana. We have demonstrated that these genomes can be cloned in S. cerevisiae as easily as those of P. tricornutum, but they are less stable when propagated in E. coli. Specifically, after approximately 60 generations of propagation in E. coli, 17% of cloned T. pseudonana mitochondrial genomes contained deletions compared to 0% of previously cloned P. tricornutum mitochondrial genomes. This genome instability is potentially due to the lower G+C DNA content of T. pseudonana (30%) compared to P. tricornutum (35%). Consequently, the previously established method can be applied to clone T. pseudonana’s mitochondrial genome, however, more frequent analyses of genome integrity will be required following propagation in E. coli prior to use in downstream applications. Full article
(This article belongs to the Special Issue Exploring and Designing Novel Microbes for Biotechnology)
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Review

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18 pages, 992 KiB  
Review
Gene Delivery Technologies with Applications in Microalgal Genetic Engineering
by Sergio Gutiérrez and Kyle J. Lauersen
Biology 2021, 10(4), 265; https://doi.org/10.3390/biology10040265 - 26 Mar 2021
Cited by 29 | Viewed by 7213
Abstract
Microalgae and cyanobacteria are photosynthetic microbes that can be grown with the simple inputs of water, carbon dioxide, (sun)light, and trace elements. Their engineering holds the promise of tailored bio-molecule production using sustainable, environmentally friendly waste carbon inputs. Although algal engineering examples are [...] Read more.
Microalgae and cyanobacteria are photosynthetic microbes that can be grown with the simple inputs of water, carbon dioxide, (sun)light, and trace elements. Their engineering holds the promise of tailored bio-molecule production using sustainable, environmentally friendly waste carbon inputs. Although algal engineering examples are beginning to show maturity, severe limitations remain in the transformation of multigene expression cassettes into model species and DNA delivery into non-model hosts. This review highlights common and emerging DNA delivery methods used for other organisms that may find future applications in algal engineering. Full article
(This article belongs to the Special Issue Exploring and Designing Novel Microbes for Biotechnology)
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