Modern Approaches in Molecular Genetics and Tissue Bioengineering: Role of Associated Biomarkers

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (20 December 2021) | Viewed by 1642

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Institute of Hydrobiology, Biology Centre CAS, Na Sádkách 7, 370 05 České Budějovice, Czech Republic
Interests: marine and freshwater; fish reproductive physiology and breeding

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Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany, Czech Republic
Interests: fisheries science; fish biology

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Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, Australia
Interests: biotechnology; fisheries science; fish biology

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Department of Biological and Medical Sciences (Faculty of Pharmacy Hradec Králové), Charles University, Prague, Czech Republic
Interests: biotechnology; cardiovascular system & cardiology; microbiology; pharmacology toxicology

Special Issue Information

Dear Colleagues,

There is a wide range of advanced approaches in molecular genetics and bioengineering from cell signaling and functional tissue engineering to cell–matrix interaction and tissue structure–function. It is believed that biomarkers can provide a powerfully dynamic method to identify and diagnose various disorders and diseases in clinical trials. Biomarkers can also reflect the entire spectrum of disease from the earliest manifestations to the terminal stages. The application of biomarkers in the diagnosis and management of several diseases, such as cardiovascular disease, infections, immunological and genetic disorders, and cancer are well known. Their use in research has grown out of the need to have a more direct measurement of exposures in the causal pathway of disease that is free from recall bias and that can also have the potential of providing information on the absorption and metabolism of the exposures. Recent studies on biomarkers have demonstrated their potential roles in molecular biology and laboratory technology. Therefore, applying novel molecular methods to determine molecular biomarkers can be regarded as a strong procedure in disease prevention, diagnosis, and management.

Dr. Amin Golpour
Dr. Hadiseh Dadras
Dr. Komeil Razmi
Dr. Samira Eissazadeh
Guest Editors

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Keywords

  • biomarker
  • cell
  • biology
  • molecular genetics
  • tissue

Published Papers (1 paper)

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Research

9 pages, 1893 KiB  
Article
The Topical Effect of rhGDF-5 Embedded in a Collagen–Gelatin Scaffold for Accelerated Wound Healing
by Wiebke Eisler, Manuel Held, Afshin Rahmanian-Schwarz, Jennifer Schiefer, Shahab Rahmanian, Adrien Daigeler and Jan-Ole Baur
Appl. Sci. 2022, 12(2), 867; https://doi.org/10.3390/app12020867 - 15 Jan 2022
Viewed by 1235
Abstract
The application of exogenous growth factors such as the recombinant human growth and differentiation factor 5 (rhGDF-5) represents a major research topic with great potential for the treatment of complex wounds. In a randomized, controlled minipig study, the topical effect of rhGDF-5 on [...] Read more.
The application of exogenous growth factors such as the recombinant human growth and differentiation factor 5 (rhGDF-5) represents a major research topic with great potential for the treatment of complex wounds. In a randomized, controlled minipig study, the topical effect of rhGDF-5 on full-thickness skin defects was evaluated. A total of 60 deep dermal wounds were either treated with rhGDF-5 embedded in an innovative collagen scaffold or another commonly used collagen matrix or left untreated. Wound healing was analyzed by planimetric analysis to determine wound closure over time. After 21 days, the areas of the initial wounds were excised, and the newly formed tissue was examined histologically. In comparison to untreated wounds, all examined matrices accelerated dermal wound healing. The largest acceleration of wound healing was seen with the high-dose rhGDF-5-treated wounds, which, compared to the untreated wounds, accelerated wound healing by 2.58 days, improved the neoepidermal thickness by 32.40 µm, and increased the epidermal cell density by 44.88 cells. The innovative collagen scaffold delivered rhGDF-5 adequately, served as a template to guide proliferating and restructuring cells, and accelerated wound healing. Thus, this composite product offers a novel tool for developing effective wound dressings in regenerative medicine. Full article
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