Recent Progresses in Cell Imaging: Current State-of-the-Art and Perspectives

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (20 April 2022) | Viewed by 2540

Special Issue Editors


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Guest Editor
Biomedical Engineering Centre, Institute of Optoelectronics, Military University of Technology, 00-908 Warsaw, Poland
Interests: stem cells; extracellular matrix; regenerative medicine; cell–cell interaction; soft X-ray microscopy; cell imaging; AFM

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Guest Editor
Biomedical Engineering Centre, Institute of Optoelectronics, Military University of Technology, 2 Kaliskiego St., 00-908 Warsaw, Poland
Interests: interaction of soft X-ray/extreme ultraviolet (SXR/EUV) radiation and low-temperature plasma with polymers; surface modification; polymeric biomaterials; scaffolds; surface bioactivation; cytocompatibility enhancement; AFM; SEM; XPS
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Guest Editor
Bioengineering Department, Lehigh University, Iaccoca Hall, 111 Research Drive, D325 Bethlehem, PA 18015, USA
Interests: extracellular vesicles; exosome; nanoparticle; stem cells; MSC-based therapy; wound healing; atomic force microscopy; regenerative medicine; cell–cell interaction; cell imaging; transmembrane proteins and cell surface receptors; drug delivery; extracellular matrix

Special Issue Information

Dear Colleagues,

Advances in medicine and life sciences would not be possible without the development of numerous microscopic techniques. Understanding living systems, especially interactions between cells and between cells and their microenvironment, is crucial in the treatment of many diseases, including cancer. In this Special Issue, we focus on the current state of the art and perspectives in microscopic techniques. Three-dimensional and living cells imaging and X-ray-based microscopies as well as confocal microscopy and super resolution techniques and others are welcome. In this Special Issue, all types of contributions, including research papers and review papers, are expected.

Dr. Agata Nowak-Stępniowska
Dr. Joanna Czwartos
Dr. Sajedehalsadat Yazdanparast
Guest Editors

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Keywords

  • live cell imaging
  • 3D imaging
  • cellular interaction
  • ECM
  • cancer cells
  • soft X-ray contact microscopy
  • X-ray-based microscopy
  • confocal microscopy
  • super resolution microscopy
  • atomic force microscopy

Published Papers (1 paper)

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Research

11 pages, 4371 KiB  
Article
M13 Phages Uptake of Gold Nanoparticles for Radio- and Thermal-Therapy and Contrast Imaging Improvement
by Lorenzo Torrisi, Letteria Silipigni, Lubomir Kovacik, Vasily Lavrentiev, Mariapompea Cutroneo, Alfio Torrisi, Laura Maria De Plano, Domenico Franco and Salvatore Guglielmino
Appl. Sci. 2021, 11(23), 11391; https://doi.org/10.3390/app112311391 - 1 Dec 2021
Cited by 1 | Viewed by 1787
Abstract
The presented work deals with the uptake of gold nanoparticles (Au NPs) by M13 phages in solutions. In particular, the Au NPs uptake modalities and their localization in the filamentous phages are evaluated and measured. Gold spherical nanoparticles (with an average diameter of [...] Read more.
The presented work deals with the uptake of gold nanoparticles (Au NPs) by M13 phages in solutions. In particular, the Au NPs uptake modalities and their localization in the filamentous phages are evaluated and measured. Gold spherical nanoparticles (with an average diameter of the order of 10 nm) are obtained by laser ablation in water with a sodium citrated surfactant. The interest of such application comes from the possibility to employ living biological structures to transport heavy metallic nanoparticles inside cells of tumoral tissues. Indeed, phages have the capability to introduce Au NPs in the proximity to the cell nucleus, increasing the efficiency of DNA destruction in the tumoral cells by employing low doses of ionizing radiation during radiotherapy and hyperthermia treatments. Several analyses and microscopy characterizations of the prepared phages samples embedding gold nanoparticles are presented, demonstrating that the presence of Au NPs increases the phages imaging contrast. Full article
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