Advances in Electroporation Systems and Applications

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Electrical, Electronics and Communications Engineering".

Deadline for manuscript submissions: 20 October 2024 | Viewed by 953

Special Issue Editor


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Guest Editor
1. Institute of High Magnetic Fields, Vilnius Gediminas Technical University, LT-10223 Vilnius, Lithuania
2. Department of Immunology, Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania
Interests: bioelectromagnetics; bioelectronics; high-power electronics; electromagnetic field effects; electroporation
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Special Issue Information

Dear Colleagues,

Electroporation is a phenomenon of biological cell membrane permeabilization triggered by a pulsed electric field; it is accompanied by the electro-transfer of target molecules inside or outside the cell. It is widely used in biomedicine, food processing, biotechnology, and other applied sciences. Depending on the electric field parameters, a variety of electroporation-mediated biological effects can be triggered, which require state-of-the-art technological platforms for pulse generation, metrology, and application. As a result, the development of electroporation systems is ongoing, and the array of applications is systemically expanded. This Special Issue is dedicated to all aspects of applied electroporation research, as well as the development of pulsed power devices.

Prof. Dr. Vitalij Novickij
Guest Editor

Manuscript Submission Information

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Keywords

  • electroporation
  • electropermeabilization
  • cell membrane permeability
  • electric field effects
  • bioelectromagnetics
  • drug delivery
  • electrochemotherapy
  • microbial inactivation
  • food processing and preservation
  • pulsed power devices
  • high-voltage generators
  • electroporators
  • irreversible electroporation
  • tissue ablation
  • lipid pores
  • electrotransformation
  • nanosecond and microsecond pulses
  • electric field processing
  • extraction of molecules
  • pulsed treatment
  • biomass processing
  • non-thermal processing

Published Papers (1 paper)

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Research

22 pages, 5518 KiB  
Article
Effects of Electroporation on the Function of Sarco/Endoplasmic Reticulum Ca2+-ATPase and Na+,K+-ATPase in H9c2 Cells
by Vid Jan, Maida Jusović and Damijan Miklavčič
Appl. Sci. 2024, 14(7), 2695; https://doi.org/10.3390/app14072695 - 22 Mar 2024
Viewed by 652
Abstract
Pulsed field ablation (PFA) is a promising new treatment for atrial fibrillation (AF), in which pulmonary vein isolation is achieved by irreversible electroporation. Electroporation causes ATP to leak through the permeabilized membrane. ATP is required both for the healing of the cell membrane [...] Read more.
Pulsed field ablation (PFA) is a promising new treatment for atrial fibrillation (AF), in which pulmonary vein isolation is achieved by irreversible electroporation. Electroporation causes ATP to leak through the permeabilized membrane. ATP is required both for the healing of the cell membrane and for the functioning of ion pumps, such as sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) or Na+,K+-ATPase (NKA), which play a key role in maintaining continuous contractions of the heart muscle. We investigated the effects of electroporation on the expression of ion pumps and possible correlations with the activation of AMPK, the main energy sensor in cells. H9c2 rat cardiac cells were exposed to either monopolar or bipolar (H-FIRE) pulses. Cells lysed 4 or 24 h after electroporation were used for mRNA and protein expression analyses. Overall, both pulse protocols caused a dose-dependent downregulation of crucial SERCA and NKA isoforms, except for NKAα2 and β3, which were upregulated after 24 h. Monopolar pulses also decreased the phosphorylation of FXYD1, which may cause an inhibition of NKA activity. Both pulse protocols caused an increased AMPK activity, which may decrease both SERCA and NKA activity via calcium/calmodulin-dependent protein kinase. Our results provide important new insights into what happens in surviving cardiomyocytes after they are exposed to PFA. Full article
(This article belongs to the Special Issue Advances in Electroporation Systems and Applications)
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