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Applied Sciences
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Applications of Enzymatic Electrochemical Biosensors
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Applications of Enzymatic Electrochemical Biosensors

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Nanotechnology and Applied Nanosciences".

Deadline for manuscript submissions: closed (31 July 2021) | Viewed by 11472

Special Issue Editors

Dr. Lukáš Richtera

E-Mail Website
Guest Editor
1. Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2. Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic
Interests: nanomaterial synthesis and characterization; nanoparticles; nanocomposites; carbon-based nanomaterials; electrochemical sensors and biosensors
Special Issues, Collections and Topics in MDPI journals
Dr. Zorica Stojanović

E-Mail Website
Guest Editor
University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
Interests: electroanalytical chemistry; chemical sensors and biosensors; electrode modification; food analysis; environmental sample analysis

Special Issue Information

Dear Colleagues,

Living organisms are complex units with different biological molecules acting as messengers which can be detected and monitored continuously using enzyme-based electrochemical biosensors in the field of healthcare and clinical diagnosis, the food industry, environmental monitoring and security from biological and chemical warfare agents (CBWA), etc. Enzymatic electrochemical biosensors are applied for the detection of different species of environmental or healthcare significance, organophosphate nerve agents, pathogenic agents, and toxins. Detection of different food contaminants is important for the food industry and food security. Cost-effective enzyme-based biosensors are required for continuous monitoring of food with greater sensitivity. In recent years, real-time monitoring of biologically significant messenger molecules has become significant to study and diagnose the physiological and pathological conditions in vivo for healthcare and diagnostic services. Effective mass screening with rapid and selective detection of CBWA is highly demanding for security and law establishment as counterterrorism measures with the increasing concern of global terrorism.

This Special Issue aims to provide an overview of enzyme-based electrochemical biosensors and their applications in different fields. The main goal is the publication of original contributions dealing with the latest trends, challenges, design and fabrication of enzymatic biosensors, usage of different nanomaterials and their incorporation in the enzymatic biosensor, development of a microfluidics or smart (nano)devices, and last but not least, their application in various research areas as well.

Dr. Lukáš Richtera
Dr. Zorica Stojanović
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Electrochemical enzymatic biosensors
  • Nanomaterials
  • Immobilization
  • Applications
  • Enzymes
  • Microfluidics

Published Papers (3 papers)

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Research

12 pages, 3353 KiB  
Article
The Effect of Synthesis Procedure on Hydrogen Peroxidase-Like Catalytic Activity of Iron Oxide Magnetic Particles
by Atripan Mukherjee, Amir M. Ashrafi, Pavel Svec, Lukáš Richtera, Jan Přibyl, Martin Brtnický, Jindrich Kynicky and Vojtěch Adam
Appl. Sci. 2020, 10(19), 6756; https://doi.org/10.3390/app10196756 - 27 Sep 2020
Cited by 2 | Viewed by 2702
Abstract
A comparative study was carried out using magnetic nanoparticles (MNPs) for the fabrication of non-enzymatic sensors for the continuous and rapid detection and monitoring of H2O2. Various MNPs, differing in terms of their synthesis procedure and modification, were synthesized [...] Read more.
A comparative study was carried out using magnetic nanoparticles (MNPs) for the fabrication of non-enzymatic sensors for the continuous and rapid detection and monitoring of H2O2. Various MNPs, differing in terms of their synthesis procedure and modification, were synthesized and characterized by different techniques. The electrochemical catalytic activity of the synthesized MNPs toward the reduction in H2O2 was investigated by cyclic voltammetry. The naked MNPs showed the highest catalytic activity among all the synthesized MNPs. The biosensor based on the naked MNPs was then applied in the determination of H2O2 using chronoamperometry. The parameters such as the applied cathodic potential and the amount of MNPs on the developed biosensor were optimized. Moreover, the analytical figures of merit, including reproducibility (RSD = 6.14%), sensitivity (m = 0.0676 µA µM−1), limit of detection (LOD) = 27.02 µmol L−1, and limit of quantification (LOQ) = 89.26 µmol L−1 of the developed biosensor indicate satisfactory analysis. Finally, MNPs were successfully utilized for the determination of H2O2 in milk. Full article
(This article belongs to the Special Issue Applications of Enzymatic Electrochemical Biosensors)
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13 pages, 2074 KiB  
Article
Simultaneous Determination of Vitamin E and Vitamin K in Food Supplements Using Adsorptive Stripping Square-Wave Voltammetry at Glassy Carbon Electrode
by Gylxhane Kastrati, Granit Jashari, Milan Sýs, Blanka Švecová, Tahir Arbneshi, Radovan Metelka, Zuzana Bílková and Lucie Korecká
Appl. Sci. 2020, 10(14), 4759; https://doi.org/10.3390/app10144759 - 10 Jul 2020
Cited by 8 | Viewed by 2588
Abstract
A new voltammetric method for the simultaneous determination of vitamin E and vitamin K present in different types of commercially available food supplements has been developed. This electroanalytical method is based on the ex situ adsorptive accumulation of these biologically active compounds onto [...] Read more.
A new voltammetric method for the simultaneous determination of vitamin E and vitamin K present in different types of commercially available food supplements has been developed. This electroanalytical method is based on the ex situ adsorptive accumulation of these biologically active compounds onto the surface of a solid glassy carbon electrode (GCE) with subsequent electrochemical detection by square-wave adsorptive stripping voltammetry in 0.01-mol L−1 HNO3 containing 0.1-mol L−1 KCl at pH 2.08. Due to reversible electrochemical reactions of phylloquinone, a subsequent voltammetric detection of both vitamins in anodic mode can be performed. Since individual forms of vitamins E and K usually exhibit nearly identical electrochemical behavior, it is therefore impossible to distinguish individual forms (quinones and tocopherols) and determine their molar concentrations in this way. Thus, the values of vitamin content were expressed as mass equivalent of phylloquinone and α-tocopherol as they are the most biologically active forms. Despite the high sensitivity, relatively short linear ranges were obtained due to the interaction (competition) of both vitamins during adsorption onto the freshly polished surface of the GCE from a 50% aqueous–acetonitrile mixture. The obtained results showed that the voltammetric approach is a very simple and low-cost analytical method that can be used in analyses of food supplements. Full article
(This article belongs to the Special Issue Applications of Enzymatic Electrochemical Biosensors)
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13 pages, 2381 KiB  
Article
Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes
by Arbër Frangu, Amir M. Ashrafi, Milan Sýs, Tahir Arbneshi, Radovan Metelka, Vojtěch Adam, Milan Vlček and Lukáš Richtera
Appl. Sci. 2020, 10(7), 2497; https://doi.org/10.3390/app10072497 - 05 Apr 2020
Cited by 10 | Viewed by 5577
Abstract
In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion® layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied [...] Read more.
In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion® layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well. Full article
(This article belongs to the Special Issue Applications of Enzymatic Electrochemical Biosensors)
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