Recent Advances and Perspectives in Engineering Plant-Derived Metabolism

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (20 April 2023) | Viewed by 2003

Special Issue Editors


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Guest Editor
Institute of Biosciences and Bioresources, IBBR-CNR, Via Universita' 133, 80055 Portici, Naples, Italy
Interests: plant specialized metabolites biosynthetic pathways, their regulation and evolution
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Institute of Biosciences and Bioresources, IBBR-CNR, Via Universita' 133, 80055 Portici, Naples, Italy
Interests: wild and cultivated solanaceae; plant derived drugs; genetic; genomic; plant specialized metabolites; metabolic engineering; plant breeding; plant functional biology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant plasticity is a plant's ability to adapt to and cope with a changing environment. This capability is mostly due to the biosynthesis of an incredible array of structurally complex, bioactive natural compounds that guarantee growth and survival. These molecules are also a source of food and feed, commodity products, and pharmaceuticals, therefore also facilitating human survival and wellbeing. Understanding the biosynthesis, regulation, and evolution of these biomolecules in plants is of great importance for increasing crop yield and quality, and enhancing the application of plant-derived compounds in several fields. This Special Issue aims to publish contributions on plant metabolism discovery, plant and microorganism engineering, and novel applications of plant compounds. All types of articles are welcome; we encourage the submission of original research reports, reviews, minireviews, and perspectives focusing on the following specific topics:

  • The biosynthesis of plant biomolecules in a changing environment.
  • The discovery of new specialized metabolites, the comprehension of their functions in plants, and their applications in human/animal health as well as novel uses in industry.
  • The biosynthesis and production of phytochemicals as a source of food/feed and for industrial uses.
  • New technologies for the prediction, elucidation, and engineering of plant metabolic pathways.
  • Novel approaches in synthetic biology for understanding and improving plant metabolism and physiology as well as powerful tools for the production and engineering of plant-derived metabolites.

Dr. Teresa Docimo
Dr. Vincenzo D’Amelia
Guest Editors

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Keywords

  • metabolic engineering
  • plant biotechnology
  • specialized metabolites
  • biosynthetic pathways
  • phytochemicals
  • human and animal health
  • novel commodities

Published Papers (1 paper)

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Review

17 pages, 4233 KiB  
Review
Overcoming Metabolic Constraints in the MEP-Pathway Enrich Salvia sclarea Hairy Roots in Therapeutic Abietane Diterpenes
by Mariaevelina Alfieri, Alfredo Ambrosone, Mariacarmela Vaccaro, Nunziatina De Tommasi and Antonietta Leone
Appl. Sci. 2022, 12(14), 7116; https://doi.org/10.3390/app12147116 - 14 Jul 2022
Cited by 1 | Viewed by 1414
Abstract
Abietane diterpenoids (e.g., carnosic acid, aethiopinone, 1-oxoaethiopinone, salvipisone, and ferruginol) synthesized in the roots of several Salvia species have proved to have promising biological activities, but their use on a large scale is limited by the very low content extracted from in vivo [...] Read more.
Abietane diterpenoids (e.g., carnosic acid, aethiopinone, 1-oxoaethiopinone, salvipisone, and ferruginol) synthesized in the roots of several Salvia species have proved to have promising biological activities, but their use on a large scale is limited by the very low content extracted from in vivo roots. In this review, we summarized our efforts and the achieved results aimed at optimizing the synthesis of these diterpenes in Salvia sclarea hairy roots by either elicitation or by modifying the expression of genes encoding enzymes of the MEP-pathway, the biosynthetic route from which they derive. Stable S. sclarea hairy roots (HRs) were treated with methyl jasmonate or coronatine, or genetically engineered, by tuning the expression of genes controlling enzymatic rate-limiting steps (DXS, DXR, GGPPS, CPPS alone or in combination), by silencing of the Ent-CPPS gene, encoding an enzyme acting at gibberellin lateral competitive route or by coordinate up-regulation of biosynthetic genes mediated by transcription factors (WRKY and MYC2). Altogether, these different approaches successfully increased the amount of abietane diterpenes in S. sclarea HRs from to 2 to 30 times over the content found in the control HR line. Full article
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