Antibiofilm Activity against Multidrug-Resistant Pathogens

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Antibiofilm Strategies".

Deadline for manuscript submissions: closed (31 March 2024) | Viewed by 2049

Special Issue Editors


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Guest Editor
Department of Biology and Ecology, Faculty of Sciences, Trg Dositeja Obradovica 3, University of Novi Sad, 21000 Novi Sad, Serbia
Interests: multidrug-resistant bacteria; bacterial biofilm; antimicrobial agents; essential oils; phytochemicals; bacteriophages; synergistic interactions; Acinetobacter baumannii

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Guest Editor
Department of Biology and Ecology, Faculty of Sciences, Trg Dositeja Obradovica 3, University of Novi Sad, 21000 Novi Sad, Serbia
Interests: bacteriophages; antimicrobial agents; bacterial biofilm; multidrug-resistant bacteria; Pseudomonas aeruginosa; phage therapy
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Special Issue Information

Dear Colleagues,

Ninety-nine percent of bacteria exist in the form of a biofilm, which represents an emerging problem in global public health, causing great concern to the population, health professionals and the scientific community. Microbial biofilms possess intrinsic resistance against conventional antibiotics and cleaning procedures, with the capability to firmly adhere to surfaces for persistent contamination. The biofilm matrix provides bacteria with additional resistance power, which enables them to tolerate harsh conditions and also resist antimicrobials, which can lead to the spread of infections such as multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria. Current conventional therapies are inadequate for the safe and effective treatment of biofilms, since high doses of antibiotics are required to eradicate biofilms, indicating that biofilm treatment needs new therapeutical strategies. These global issues strongly motivate researchers to develop novel methodologies to investigate the kinetics underlying biofilm formation, to understand the response of the biofilm to different chemical and physical treatments, and to identify biofilm-specific drugs with high-throughput screenings. This Special Issue seeks manuscript submissions that improve our understanding of the process of biofilm formation in general, novel strategies for its investigation, as well as efficient agents for its treatment. Submissions on the treatment and potential eradication of biofilms using alternative antimicrobial agents (alone and in synergistic combinations) and/or novel strategies for their investigation and combined treatment to reduce their transmission and spread are especially encouraged.

Dr. Verica Aleksic Sabo
Prof. Dr. Petar Knezevic
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • biofilm
  • microbial drug resistance
  • antibiofilm agents
  • antibiofilm strategies
  • synergistic interactions

Published Papers (1 paper)

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Research

16 pages, 17399 KiB  
Article
In Vitro Antibiofilm Activity of Resveratrol against Aeromonas hydrophila
by Ting Qin, Kai Chen, Bingwen Xi, Liangkun Pan, Jun Xie, Liushen Lu and Kai Liu
Antibiotics 2023, 12(4), 686; https://doi.org/10.3390/antibiotics12040686 - 31 Mar 2023
Cited by 7 | Viewed by 1700
Abstract
Aeromonas hydrophila is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated the effect of resveratrol on A. hydrophila [...] Read more.
Aeromonas hydrophila is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated the effect of resveratrol on A. hydrophila biofilm formation and motility. The results demonstrated that resveratrol, at sub-MIC levels, can significantly inhibit the biofilm formation of A. hydrophila, and the biofilm was decreased with increasing concentrations. The motility assay showed that resveratrol could diminish the swimming and swarming motility of A. hydrophila. Transcriptome analyses (RNA-seq) showed that A. hydrophila treated with 50 and 100 μg/mL resveratrol, respectively, presented 230 and 308 differentially expressed genes (DEGs), including 90 or 130 upregulated genes and 130 or 178 downregulated genes. Among them, genes related to flagellar, type IV pilus and chemotaxis were significantly repressed. In addition, mRNA of virulence factors OmpA, extracellular proteases, lipases and T6SS were dramatically suppressed. Further analysis revealed that the major DEGs involved in flagellar assembly and bacterial chemotaxis pathways could be regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- and LysR-Type transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, our results indicate that resveratrol can inhibit A. hydrophila biofilm formation by disturbing motility and QS systems, and can be used as a promising candidate drug against motile Aeromonad septicemia. Full article
(This article belongs to the Special Issue Antibiofilm Activity against Multidrug-Resistant Pathogens)
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