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Agronomy
Special Issues
Innovation in Detection and Management of Diseases of Vegetables and...
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Innovation in Detection and Management of Diseases of Vegetables and Fruits

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Agricultural Biosystem and Biological Engineering".

Deadline for manuscript submissions: closed (31 March 2020) | Viewed by 8947

Special Issue Editors

Prof. Dr. Maria Lodovica Gullino

E-Mail Website
Guest Editor
Centro di Competenza Agroinnova and DISAFA, University of Torino, 10124 Torino, TO, Italy
Interests: plant pathology; plant disease management; plant and food biosecurity; climate change and plant disease
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Davide Spadaro

E-Mail Website
Guest Editor
Department of Agricultural, Forest and Food Sciences (DISAFA) and AGROINNOVA (Centre of Competence for the Innovation in the Agro-Environmental Sector), University of Turin, 10124 Turin, Italy
Interests: plant pathology; crop protection; molecular diagnostics; biological control; postharvest; mycotoxins
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear colleagues,

The scope of this special issue is to provide an update on innovative detection and management tools for pathogens of vegetables and fruits. Reviews and original research articles dealing with new problems posed by well-known diseases and innovative strategies for their management or addressing new diseases for which it is necessary to develop specific protection tools or modify currently applied management strategies fit the scope of this issue.

This Issue will be released during the International Year of Plant Health (IYPH) and will help rising awareness of the importance of plant health for a sustainable development of farming systems. Integrated disease management intends to manage plant diseases by assembling innovative prevention, detection and control tools. Most practices designed to improve soil health help to guarantee the plant health. The use of healthy seed or propagation material is an effective tool to prevent pathogens. Rapid and reliable diagnostic methods for plant pathogens allow for a rational and efficient use of plant protection products. New molecular techniques (qPCR, digital PCR, microarray) have been developed to pathogen detection. High throughput sequencing is having an enormous impact on plant health. The use of field techniques, such as LAMP and portable platforms, is promising to early and quickly detect pathogens. Innovative biobased products, such as antagonistic microroganisms or botanicals, permit an environmentally sustainable approach to disease management. The choice of the appropriate plant disease management strategy should not only integrate the impact on the soil and crop health, but also on the agricultural and non-agricultural environments, the natural resources, and human health.

Prof. Dr. Maria Lodovica Gullino
Prof. Dr. Davide Spadaro
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant pathogens
  • biological and integrated control
  • molecular diagnostics
  • botanicals
  • LAMP
  • qPCR
  • biocontrol agents

Published Papers (2 papers)

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Research

16 pages, 274 KiB  
Article
New Molecular Tool for a Quick and Easy Detection of Apple Scab in the Field
by Sara Franco Ortega, Simona Prencipe, Maria Lodovica Gullino and Davide Spadaro
Agronomy 2020, 10(4), 581; https://doi.org/10.3390/agronomy10040581 - 18 Apr 2020
Cited by 11 | Viewed by 3116
Abstract
Venturia inaequalis, an agent of apple scab, is the most important pathogen of Malus x domestica. Control measures against this pathogen rely on intensive phytosanitary programs based on predictive models to identify the meteorological conditions conducive to the primary infection. The detection [...] Read more.
Venturia inaequalis, an agent of apple scab, is the most important pathogen of Malus x domestica. Control measures against this pathogen rely on intensive phytosanitary programs based on predictive models to identify the meteorological conditions conducive to the primary infection. The detection of the pathogen in field, both in naturally infected symptomatic and asymptomatic leaves, is desirable. Loop-mediated isothermal amplification (LAMP) assays are profitable molecular diagnostic tools for the direct detection of pathogens in field. A LAMP assay for V. inaequalis has been designed on the elongation factor 1-alpha sequence. The validation of the LAMP assay was carried out following the international EPPO standard PM 7/98 in terms of specificity, sensitivity, repeatability and reproducibility. Specificity testing was performed using target and non-target species, such as phylogenetically related Venturia species and other pathogens commonly found in apple, resulting in positive amplification only for the target with a time to positive ranging from 20 to 30 min. Sensitivity testing was performed with serial dilutions of DNA of the target and by artificial inoculation of young apple leaves. The reliability of the LAMP assay as an early-detection tool and its user-friendly application make it suitable for the diagnosis of apple scab in the field. Full article
(This article belongs to the Special Issue Innovation in Detection and Management of Diseases of Vegetables and Fruits)
15 pages, 4188 KiB  
Article
Species Diversity in Colletotrichum Causing Anthracnose of Aromatic and Ornamental Lamiaceae in Italy
by Vladimiro Guarnaccia, Giovanna Gilardi, Ilaria Martino, Angelo Garibaldi and Maria Lodovica Gullino
Agronomy 2019, 9(10), 613; https://doi.org/10.3390/agronomy9100613 - 05 Oct 2019
Cited by 30 | Viewed by 5439
Abstract
Species of Colletotrichum are considered important plant pathogens, saprobes, and endophytes on a wide range of host plants. In Italy, several Colletotrichum species have been reported in glasshouse environments. In this study, we have explored the occurrence, diversity, and pathogenicity of Colletotrichum spp. [...] Read more.
Species of Colletotrichum are considered important plant pathogens, saprobes, and endophytes on a wide range of host plants. In Italy, several Colletotrichum species have been reported in glasshouse environments. In this study, we have explored the occurrence, diversity, and pathogenicity of Colletotrichum spp. associated with aromatic and ornamental plants belonging to the Lamiaceae family. Surveys were carried out during the 2011–2018 period in Liguria and Piedmont, Italy. A total of 19 Colletotrichum isolates were collected from symptomatic leaves and seeds of Ocimum basilicum (basil), Origanum vulgare (oregano) and different Salvia spp. A multi-locus phylogeny was established based on the basis of four genomic loci (ITS, GAPDH, ACT and TUB2). The aggressiveness of selected, representative isolates were tested. Colletotrichum isolates were identified as being members of three major species complexes: C. acutatum, C. destructivum, and C. gloeosporioides. Colletotrichum fioriniae, C. bryonicola, and C. fructicola were found in association with leaf lesions on Salvia leucantha, S. nemorosa, and S. greggii, respectively. Colletotrichum nigrum was isolated from twig lesions of S. greggii. Moreover, C. fioriniae and C. ocimi were found to be responsible for causing leaf anthracnose of oregano and basil, respectively. All the tested isolates were pathogenic and reproduced identical symptoms to those observed in commercial glasshouses. The present study improves our understanding of Colletotrichum species associated with several hosts belonging to the Lamiaceae family, which are cultivated extensively throughout Italy for different purpose, and provides information that may be useful for an effective disease management program. Full article
(This article belongs to the Special Issue Innovation in Detection and Management of Diseases of Vegetables and Fruits)
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