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Agriculture
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Detection and Identification of Transgenic Organisms in Agriculture
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Detection and Identification of Transgenic Organisms in Agriculture

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Agricultural Product Quality and Safety".

Deadline for manuscript submissions: closed (10 December 2022) | Viewed by 16521

Special Issue Editors

Dr. Yuhua Wu

E-Mail Website
Guest Editor
Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China
Interests: GMO; genome-edited plants; detection; DNA; quantification; real-time PCR; digital PCR; reference materials
Dr. Hongfei Gao

E-Mail Website
Guest Editor
Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China
Interests: genetically modified organisms; chemiluminescence immunoassay; electrochemistry immunosensor; electrochemiluminescence immunosensor; immunochromatography assay; nanomaterials; electrochemistry DNA sensor; isothermal amplification; point-of-care testing

Special Issue Information

Dear Colleagues,

The rapid development of genetically modified organisms (GMO) has produced huge benefits. To guarantee the healthy development of biotech organisms, many countries/regions stipulate GMO regulations to monitor GMO planting and application in food and feed. The implementation of GMO regulations requires establishing GMO detection technology that can screen GMO ingredients, identify GM events, and quantify GMO contents. The emergence of genome-edited organisms poses a huge challenge to the development of regulation-compliant detection technology for monitoring and testing.

This Special Issue focuses on the development and assessment of GMO detection methods in the screening, identification and quantification of GMOs. This SI on the Detection and Identification of Transgenic Organisms will include interdisciplinary studies embracing agriculture within the disciplines of biochemistry, molecular biology, and omics. Research articles will cover a broad range of accurate quantification technologies, fast detection technology, high-throughput technology and technology focusing on detecting genome-edited organisms. All types of articles are welcome.

Dr. Yuhua Wu
Dr. Hongfei Gao
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agriculture is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • transgenic organisms
  • genome editing
  • protein
  • biosensor
  • DNA
  • PCR
  • sequencing
  • identification
  • quantification
  • fast detection

Published Papers (6 papers)

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Research

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11 pages, 2086 KiB  
Article
Establishment of an ELISA Method for Quantitative Detection of PAT/pat in GM Crops
by Weixiao Liu, Lixia Meng, Xuri Liu, Chao Liu and Wujun Jin
Agriculture 2022, 12(9), 1400; https://doi.org/10.3390/agriculture12091400 - 5 Sep 2022
Cited by 2 | Viewed by 2383
Abstract
The phosphinothricin N-acetyltransferase gene (pat) is widely used to confer resistance to the herbicide phosphinothricin for genetically modified (GM) crops. A quantitative sandwich enzyme-linked immunosorbent assay (ELISA) is developed to detect PAT/pat in GM crops. Two anti-PAT/pat monoclonal antibodies (mAbs), 1F5-1F2 [...] Read more.
The phosphinothricin N-acetyltransferase gene (pat) is widely used to confer resistance to the herbicide phosphinothricin for genetically modified (GM) crops. A quantitative sandwich enzyme-linked immunosorbent assay (ELISA) is developed to detect PAT/pat in GM crops. Two anti-PAT/pat monoclonal antibodies (mAbs), 1F5-1F2 and 1B6-2D3, with titers of 1:1,024,000 and 1:896,000, respectively, against overexpressed His-PAT/pat, were screened out, raised, and characterized. An ELISA method was established with the 1F5-2F2 mAb for capture and the biotin-labeled 1B6-2D3 mAb for detection of PAT/pat. The linear detection range of the method was approximately 1.5625–12.5 ng/mL, with a sensitivity of 0.085 ng/mL and a coefficient of variation (CV) less than 5.0%. No cross-reactivity was found with other herbicide resistance proteins, especially PAT/bar. The established sensitive and specific ELISA was successfully applied in the detection of PAT/pat expression in GM crops. Full article
(This article belongs to the Special Issue Detection and Identification of Transgenic Organisms in Agriculture)
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9 pages, 2207 KiB  
Communication
Rapid Identification of HSA Genetically Modified Goats by Combining Recombinase Polymerase Amplification (RPA) with Lateral Flow Dipstick (LFD)
by Qiuju Su, Kaifeng Guan, Xiang Zhou, Yang Zhou and Bang Liu
Agriculture 2022, 12(7), 927; https://doi.org/10.3390/agriculture12070927 - 27 Jun 2022
Viewed by 1620
Abstract
Genetically modified (GM) animals have attracted considerable attention and faced challenges. Human serum albumin (HSA) GM goats have been used to produce goat milk with serum albumin from humans, which has shown great potential in the market. Establishing an accurate method [...] Read more.
Genetically modified (GM) animals have attracted considerable attention and faced challenges. Human serum albumin (HSA) GM goats have been used to produce goat milk with serum albumin from humans, which has shown great potential in the market. Establishing an accurate method to distinguish goats with a genome modified by HSA has become necessary. Here, we established a platform to detect HSA GM goats by combining the advantages of recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) strategies. The whole detection process could be completed within 1 h, obtaining a direct result that could be visualized by a characteristic red band after a quick amplification under a constant temperature of 42 °C in the RPA experiments. Moreover, the GM goats could be identified with a detection limit of 0.1 ng using our method. Therefore, this study provided a rapid and convenient RPA-LFD method for the immediate detection of HSA GM goats. This will be useful for the identification of HSA GM goats, which may be used to distinguish the mixture of GM mutton and normal mutton. Full article
(This article belongs to the Special Issue Detection and Identification of Transgenic Organisms in Agriculture)
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16 pages, 683 KiB  
Article
Compositional and Animal Feeding Assessments of a Novel Herbicide-Tolerant Maize Variety
by Xiaoxing Yu, Yaohui Huang, Xiaoyun Chen, Ziying Zhou, Zhicheng Shen and Pengfei Wang
Agriculture 2022, 12(6), 808; https://doi.org/10.3390/agriculture12060808 - 3 Jun 2022
Cited by 4 | Viewed by 1862
Abstract
ZDAX5 is a variety of herbicide-tolerant maize that contains the modified P450-N-Z1 gene isolated from Cynodon dactylon (L.) Pers. and the cp4 epsps gene isolated from the Agrobacterium tumefaciens strain CP4 and exhibits high tolerances to flazasulfuron and glyphosate under field conditions. Once [...] Read more.
ZDAX5 is a variety of herbicide-tolerant maize that contains the modified P450-N-Z1 gene isolated from Cynodon dactylon (L.) Pers. and the cp4 epsps gene isolated from the Agrobacterium tumefaciens strain CP4 and exhibits high tolerances to flazasulfuron and glyphosate under field conditions. Once ZDAX5 corn is available on the market, the evolution of herbicide-resistant weeds will be delayed by applying glyphosate and flazasulfuron to corn fields. Prior to commercialization, it is critical to assess the safety of ZDAX5 maize. Compositional analysis and feed consumption studies in rodents are an important consideration in the safety assessment of genetically modified crops. The nutritional components of ZDAX5 were analyzed and compared with those of its non-transgenic counterpart. The data showed that all the analyzed components in the herbicide-tolerant maize plants were substantially equivalent to those of its non-transgenic counterpart. Furthermore, most of the measured values from ZDAX5 were within the range of values reported for other commercial maize varieties. The sub-chronic feeding trial was carried out with grains from GM, and non-GM maize were independently added into rodent diets at concentrations of 12.5%, 25% and 50%. As a control, another set of rats was fed with a marketed diet. At the end of the 90-day feeding study, no negative effects associated with the consumption of GM maize were found. These results indicate that the herbicide-tolerant maize ZDAX5 is as nutritious and safe as non-transgenic maize. Full article
(This article belongs to the Special Issue Detection and Identification of Transgenic Organisms in Agriculture)
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15 pages, 683 KiB  
Article
Genetically Modified Crops in Romania before and after the Accession of the European Union
by Maria-Mihaela Antofie and Camelia Sand-Sava
Agriculture 2022, 12(4), 458; https://doi.org/10.3390/agriculture12040458 - 25 Mar 2022
Cited by 3 | Viewed by 3842
Abstract
Modern biotechnology entered the world science arena after 1972, and in less than 22 years the first genetically modified crop was placed on the market. The analysis of relevant biosafety portals and official and scientific documents was applied in this study to reveal [...] Read more.
Modern biotechnology entered the world science arena after 1972, and in less than 22 years the first genetically modified crop was placed on the market. The analysis of relevant biosafety portals and official and scientific documents was applied in this study to reveal positive and negative issues of the Romanian biosafety framework before and after the European Union (EU) accession. The success in cultivating highly productive GM soybean was rapidly embraced by Romania between 1999 and 2005. Before 2007, Romania was cited among the Mega Biotech Countries, with a cultivation of 87.500 ha. After the accession to the European Union, Romania stopped any GM crop cultivation. There was an immense effort to harmonize with the EU regulatory framework between 2005 and 2007. The monitoring system for GM crops has been in place since 2007. Public research on aspects of GMOs started before 1996 and continues to develop. The analysis of our results supports the idea that Romania is a perfect example of a country committed to embracing GM crop cultivation for 7 years, followed by almost complete phasing out of GM crop cultivation to comply with all requirements for EU accession in less than 1 year. Full article
(This article belongs to the Special Issue Detection and Identification of Transgenic Organisms in Agriculture)
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12 pages, 1216 KiB  
Article
A Label-Free Electrochemical Impedance Genosensor Coupled with Recombinase Polymerase Amplification for Genetically Modified Maize Detection
by Dandan Cui, Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Xiaohong Yan, Ping Shen, Hongfei Gao and Gang Wu
Agriculture 2022, 12(4), 454; https://doi.org/10.3390/agriculture12040454 - 23 Mar 2022
Cited by 4 | Viewed by 3447
Abstract
As the cultivation scale of genetically modified (GM) crops strongly increases, a convenient DNA assay is highly demanded in resource-limited areas. A label-free electrochemical impedance (EI) genosensor using gold carbon dots (GCDs) was developed with easy-to-use portable device. GCDs were used to modify [...] Read more.
As the cultivation scale of genetically modified (GM) crops strongly increases, a convenient DNA assay is highly demanded in resource-limited areas. A label-free electrochemical impedance (EI) genosensor using gold carbon dots (GCDs) was developed with easy-to-use portable device. GCDs were used to modify screen-printed carbon electrode and immobilize capture probes by conducting a simple protocol. After the amplification products anchored on the sensor surface via hybridization reactions, the EI signal increased due to the formation of biocomplex hampering the interfacial electron transfer. Under the optimal conditions, the proposed genosensor coupled with recombinase polymerase amplification (RPA) could detect maize Ruifeng12-5 in a linear range of 0.10–5.0% with a detection limit of 0.10%. In addition, combined with a one-step extraction and RPA amplification, the proposed sensor device can be applied in resource-limited laboratories without expensive instruments or professionals. Therefore, the developed method provides an easy-to-use and sensitive platform for GM organism detection. Full article
(This article belongs to the Special Issue Detection and Identification of Transgenic Organisms in Agriculture)
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11 pages, 1964 KiB  
Perspective
Advanced Technologies in On-Site Detection of Genetically Modified Products
by Jiale Li, Jie Li, Shenghao Lin, Longjiao Zhu, Xiangyang Li and Wentao Xu
Agriculture 2022, 12(6), 888; https://doi.org/10.3390/agriculture12060888 - 20 Jun 2022
Viewed by 2464
Abstract
Transgenic technology is significantly impacting life today. However, with the advancement of genetically modified technologies and the success of genetically modified product commercialization, new challenges have arisen for associated detecting technologies. The need for fast, precise, and portable systems for the on-site detection [...] Read more.
Transgenic technology is significantly impacting life today. However, with the advancement of genetically modified technologies and the success of genetically modified product commercialization, new challenges have arisen for associated detecting technologies. The need for fast, precise, and portable systems for the on-site detection of genetically modified products has increased dramatically in recent years. This perspective examined the currently available technological support for portable immune biosensing, discussed a portable detection device for ultrafast PCR, and an on-site detection biosensor based on functional nucleic acid and superior detection devices in the field. Moreover, the on-site sequencing of genetically modified organisms was mentioned briefly. Lastly, the future outlook of genetically modified products detection was concluded and discussed in order to provide a comprehensive reference for future research and development in related fields. Full article
(This article belongs to the Special Issue Detection and Identification of Transgenic Organisms in Agriculture)
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