Astaxanthin Supplementation Reduces Subjective Markers of Muscle Soreness following Eccentric Exercise in Resistance-Trained Men
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Subjects
Procedures
4.2. Exercise-Induced Muscle Damage (Trials 1 and 3)
4.3. Performance Trials (Trials 2 & 4)
4.4. Supplementation Schedule
4.5. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Bloomer, R.J.; Fry, A.; Schilling, B.; Chiu, L.; Hori, N.; Weiss, L. Astaxanthin Supplementation Does Not Attenuate Muscle Injury Following Eccentric Exercise in Resistance-Trained Men. Int. J. Sport. Nutr. Exerc. Metab. 2005, 15, 401–412. [Google Scholar] [CrossRef]
- Nosaka, K.; Newton, M. Concentric or eccentric training effect on eccentric exercise-induced muscle damage. Med. Sci. Sport. Exerc. 2002, 34, 63–69. [Google Scholar] [CrossRef] [Green Version]
- Armstrong, R.B.; Warren, G.L.; Warren, J.A. Mechanisms of Exercise-Induced Muscle Fibre Injury. Sport. Med. 1991, 12, 184–207. [Google Scholar] [CrossRef] [PubMed]
- Cheung, K.; Hume, P.A.; Maxwell, L. Delayed onset muscle soreness. Sport. Med. 2003, 33, 145–164. [Google Scholar] [CrossRef] [PubMed]
- Lee, J.; Goldfarb, A.H.; Rescino, M.H.; Hegde, S.; Patrick, S.; Apperson, K. Eccentric exercise effect on blood oxidative-stress markers and delayed onset of muscle soreness. Med. Sci. Sport. Exerc. 2002, 34, 443–448. [Google Scholar] [CrossRef]
- Maughan, R.J.; Donnelly, A.E.; Gleeson, M.; Whiting, P.H.; Walker, K.A.; Clough, P.J. Delayed-onset muscle damage and lipid peroxidation in man after a downhill run. Muscle Nerve 1989, 12, 332–336. [Google Scholar] [CrossRef]
- Barbosa, M.L.; de Meneses, A.-A.P.M.; de Aguiar, R.P.S.; de Castro e Sousa, J.M.; de Carvalho Melo Cavalcante, A.A.; Maluf, S.W. Oxidative stress, antioxidant defense and depressive disorders: A systematic review of biochemical and molecular markers. Neurol. Psychiatry Brain Res. 2020, 36, 65–72. [Google Scholar] [CrossRef]
- Li, D.; Ding, Z.; Du, K.; Ye, X.; Cheng, S. Reactive Oxygen Species as a Link between Antioxidant Pathways and Autophagy. Oxidative Med. Cell. Longev. 2021, 2021, 5583215. [Google Scholar] [CrossRef]
- Close, G.L.; Ashton, T.; McArdle, A.; MacLaren, D.P. The emerging role of free radicals in delayed onset muscle soreness and contraction-induced muscle injury. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2005, 142, 257–266. [Google Scholar] [CrossRef]
- Witt, E.H.; Reznick, A.Z.; Viguie, C.A.; Starke-Reed, P.; Packer, L. Exercise, Oxidative Damage and Effects of Antioxidant Manipulation. J. Nutr. 1992, 122, 766–773. [Google Scholar] [CrossRef]
- Tsai, K.; Hsu, T.G.; Hsu, K.M.; Cheng, H.; Liu, T.Y.; Hsu, C.F.; Kong, C.W. Oxidative DNA Damage in Human Peripheral Leukocytes Induced by Massive Aerobic Exercise. Free Radic. Biol. Med. 2001, 31, 1465–1472. [Google Scholar]
- Cleak, M.; Eston, R. Delayed onset muscle soreness: Mechanisms and management. J. Sport. Sci. 1992, 10, 325–341. [Google Scholar] [CrossRef] [PubMed]
- Reno, A.M.; Green, M.; Killen, L.G.; O’Neal, E.K.; Pritchett, K.; Hanson, Z. Effects of Magnesium Supplementation on Muscle Soreness and Performance. J. Strength Cond. Res. 2022, 36, 2198–2203. [Google Scholar] [CrossRef] [PubMed]
- Connolly, D.A.; Sayers, S.E.; McHugh, M.P. Treatment and prevention of delayed onset muscle soreness. J. Strength Cond. Res. 2003, 17, 197–208. [Google Scholar]
- Pisoschi, A.M.; Pop, A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur. J. Med. Chem. 2015, 97, 55–74. [Google Scholar] [CrossRef] [PubMed]
- Beaton, L.J.; Allan, D.A.; Tarnopolsky, M.A.; Tiidus, P.M.; Phillips, S.M. Contraction-induced muscle damage is unaffected by vitamin E supplementation. Med. Sci. Sport. Exerc. 2002, 34, 798–805. [Google Scholar] [CrossRef] [PubMed]
- Close, G.L.; Ashton, T.; Cable, N.; Doran, D.; Holloway, C.; McArdle, F.; MacLaren, D.P.M. Ascorbic acid supplementation does not attenuate post-exercise muscle soreness following muscle-damaging exercise but may delay the recovery process. Br. J. Nutr. 2006, 95, 976–981. [Google Scholar] [CrossRef]
- Righi, N.C.; Schuch, F.B.; De Nardi, A.T.; Pippi, C.M.; De Almeida Righi, G.; Puntel, G.O.; Da Silva, A.M.V.; Signori, L.U. Effects of vitamin C on oxidative stress, inflammation, muscle soreness, and strength following acute exercise: Meta-analyses of randomized clinical trials. Eur. J. Nutr. 2020, 59, 2827–2839. [Google Scholar] [CrossRef]
- Bloomer, R.J. Effects of Antioxidant Therapy in Women Exposed to Eccentric Exercise. Int. J. Sport Nutr. Exerc. Metab. 2004, 14, 377–388. [Google Scholar] [CrossRef]
- Ives, S.J.; Bloom, S.; Matias, A.; Morrow, N.; Martins, N.; Roh, Y.; Ebenstein, D.; O’brien, G.; Escudero, D.; Brito, K.; et al. Effects of a combined protein and antioxidant supplement on recovery of muscle function and soreness following eccentric exercise. J. Int. Soc. Sport. Nutr. 2017, 14, 21. [Google Scholar] [CrossRef] [Green Version]
- Guerin, M.; Huntley, M.E.; Olaizola, M. Haematococcus astaxanthin: Applications for human health and nutrition. Trends Biotechnol. 2003, 21, 210–216. [Google Scholar] [CrossRef]
- Hecht, K.A.; Schnackenberg, J.; Nair, A.; Lignell, Å. Astaxanthin for improved muscle function and enhanced physical performance. In Global Perspectives on Astaxanthin; Academic Press: Cambridge, MA, USA, 2021; pp. 447–467. [Google Scholar] [CrossRef]
- Hussein, G.; Sankawa, U.; Goto, H.; Matsumoto, A.K.; Watanabe, H. Astaxanthin, a Carotenoid with Potential in Human Health and Nutrition. J. Nat. Prod. 2006, 69, 443–449. [Google Scholar] [CrossRef] [PubMed]
- McAllister, M.J.; Mettler, J.A.; Patek, K.; Butawan, M.; Bloomer, R.J. Astaxanthin Supplementation Increases Glutathione Concentrations but Does Not Impact Fat Oxidation During Exercise in Active Young Men. Int. J. Sport Nutr. Exerc. Metab. 2022, 32, 8–15. [Google Scholar] [CrossRef]
- Baralic, I.; Djordjevic, B.; Dikic, N.; Kotur-Stevuljevic, J.; Spasic, S.; Jelic-Ivanovic, Z.; Radivojevic, N.; Andjelkovic, M.; Pejic, S. Effect of Astaxanthin Supplementation on Paraoxonase 1 Activities and Oxidative Stress Status in Young Soccer Players. Phytother. Res. 2012, 27, 1536–1542. [Google Scholar] [CrossRef]
- Waldman, H.S.; Bryant, A.R.; Parten, A.L.; Grozier, C.D.; McAllister, M.J. Astaxanthin Supplementation Does Not Affect Markers of Muscle Damage or Inflammation After an Exercise-Induced Muscle Damage Protocol in Resistance-Trained Males. J. Strength Cond. Res. 2023. [Google Scholar] [CrossRef] [PubMed]
- Malm, C.; Nyberg, P.; Engström, M.; Sjödin, B.; Lenkei, R.; Ekblom, B.; Lundberg, I. Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies. J. Physiol. 2000, 529, 243–262. [Google Scholar] [CrossRef] [PubMed]
- Kanda, K.; Sugama, K.; Hayashida, H.; Sakuma, J.; Kawakami, Y.; Miura, S.; Yoshioka, H.; Mori, Y.; Suzuki, K. Eccentric exercise-induced delayed-onset muscle soreness and changes in markers of muscle damage and inflammation. Exerc. Immunol. Rev. 2013, 19, 72–85. [Google Scholar] [PubMed]
- Basham, S.A.; Waldman, H.S.; Krings, B.M.; Lamberth, J.; Smith, J.W.; McAllister, M.J. Effect of Curcumin Supplementation on Exercise-Induced Oxidative Stress, Inflammation, Muscle Damage, and Muscle Soreness. J. Diet. Suppl. 2019, 17, 401–414. [Google Scholar] [CrossRef]
- Nicol, L.M.; Rowlands, D.S.; Fazakerly, R.; Kellett, J. Curcumin supplementation likely attenuates delayed onset muscle soreness (DOMS). Eur. J. Appl. Physiol. 2015, 115, 1769–1777. [Google Scholar] [CrossRef]
- Close, G.L.; Ashton, T.; Cable, N.; Doran, D.; MacLaren, D.P.M. Eccentric exercise, isokinetic muscle torque and delayed onset muscle soreness: The role of reactive oxygen species. Eur. J. Appl. Physiol. 2004, 91, 615–621. [Google Scholar] [CrossRef]
- Lenn, J.; Uhl, T.; Mattacola, C.; Boissonneault, G.; Yates, J.; Ibrahim, W.; Bruckner, G. The effects of fish oil and isoflavones on delayed onset muscle soreness. Med. Sci. Sport. Exerc. 2002, 34, 1605–1613. [Google Scholar] [CrossRef] [PubMed]
- McFarlin, B.K.; Venable, A.S.; Henning, A.L.; Sampson, J.N.B.; Pennel, K.; Vingren, J.L.; Hill, D.W. Reduced inflammatory and muscle damage biomarkers following oral supplementation with bioavailable curcumin. BBA Clin. 2016, 5, 72–78. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Laurent, C.M.; Green, J.M.; Bishop, P.A.; Sjökvist, J.; Schumacker, R.E.; Richardson, M.T.; Curtner-Smith, M. A Practical Approach to Monitoring Recovery: Development of a Perceived Recovery Status Scale. J. Strength Cond. Res. 2011, 25, 620–628. [Google Scholar] [CrossRef] [PubMed]
- Yates, J.W.; Armbruster, W.J. Concentric and eccentric strength loss and recovery following exercise induced muscle soreness. Int. J. Sport. Med. 1990, 11, 403. [Google Scholar]
- Atalay, M.; Lappalainen, J.; Sen, C.K. Dietary Antioxidants for the Athlete. Curr. Sport. Med. Rep. 2006, 5, 182–186. [Google Scholar] [CrossRef]
- Wong, S.K.; Ima-Nirwana, S.; Chin, K. Effects of astaxanthin on the protection of muscle health. Exp. Ther. Med. 2020, 20, 2941–2952. [Google Scholar] [CrossRef]
- Brown, D.R.; Warner, A.R.; Deb, S.K.; Gough, L.A.; Sparks, S.A.; McNaughton, L.R. The effect of astaxanthin supplementation on performance and fat oxidation during a 40 km cycling time trial. J. Sci. Med. Sport 2021, 24, 92–97. [Google Scholar] [CrossRef]
- Talbott, J.; Hantla, D.; Talbott, S.M. Effect of Astaxanthin Supplementation on Cardiorespiratory Function in Runners. Med. Sci. Sport. Exerc. 2017, 49, 941. [Google Scholar] [CrossRef]
- Earnest, C.; Lupo, M.; White, K.; Church, T. Effect of Astaxanthin on Cycling Time Trial Performance. Int. J. Sport. Med. 2011, 32, 882–888. [Google Scholar] [CrossRef]
- Brown, D.R.; Gough, L.A.; Deb, S.K.; Sparks, S.A.; McNaughton, L.R. Astaxanthin in Exercise Metabolism, Performance and Recovery: A Review. Front. Nutr. 2018, 4, 76. [Google Scholar] [CrossRef] [Green Version]
- Kluwer, W. Acsm’s Guidelines for Exercise Testing and Prescription, 10th ed.; American College of Sports Medicine: Indianapolis, IN, USA, 2021. [Google Scholar]
- Robertson, R.J.; Goss, F.L.; Rutkowski, J.; Lenz, B.; Dixon, C.; Timmer, J.; Frazee, K.; Dube, J.; Andreacci, J. Concurrent Validation of the OMNI Perceived Exertion Scale for Resistance Exercise. Med. Sci. Sport. Exerc. 2003, 35, 333–341. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Rüfer, C.E.; Moeseneder, J.; Briviba, K.; Rechkemmer, G.; Bub, A. Bioavailability of astaxanthin stereoisomers from wild (Oncorhynchus spp.) and aquacultured (salmo salar) salmon in healthy men: A randomised, double-blind study. Br. J. Nutr. 2008, 99, 1048–1054. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Placebo (n = 9) | Astaxanthin (n = 10) | |
---|---|---|
Age | 23.1.0 ± 2.0 | 22.0 ± 2.4 |
Body mass (kg) | 93.9 ± 13.8 | 91.6 ± 9.8 |
Height (cm) | 180.2 ± 4.0 | 178.6 ± 6.0 |
Body fat (%) | 22.4 ± 5.8 | 22.6 ± 6.6 |
24-h Pre | 24-h Post | 36-h Pre | 36-h Post | 48-h Pre | 48-h Post | |
---|---|---|---|---|---|---|
SORE (0–6) AX (n = 10) * PLA (n = 9) | 3.3 ± 0.8 2.8 ± 1.5 | 1.4 ± 1.1 3.0 ± 1.4 | 2.8 ± 1.2 2.3 ± 1.2 | 1.9 ± 1.2 2.5 ± 1.6 | ||
VAS (mm) AX (n = 10) ** PLA (n = 9) | 69.9 ± 62.3 62.3 ± 32.1 | 28.2 ± 18.8 70.9 ± 40.1 | 68.0 ± 27.5 56.9 ± 36.7 | 28.2 ± 19.6 63.7 ± 34.1 | 63.1 ± 31.2 55.9 ± 31.4 | 24.9 ± 19.8 54.8 ± 30.0 |
Pre | Post | Pre | Post | |
---|---|---|---|---|
AX (n = 10) | AX (n = 10) | PLA (n = 9) | PLA (n = 9) | |
1RM (kg) | 352.9 ± 75.2 | 379.3 ± 75.2 | 387.1 ± 66.5 | 401.5 ± 67.3 |
65% 1RM (reps) | 19 ± 6 | 20 ± 8 | 21 ± 7 | 23 ± 12 |
70% 1RM (reps) | 13 ± 5 | 12 ± 4 | 16 ± 5 | 16 ± 7 |
75% 1RM (reps) | 10 ± 3 | 9 ± 4 | 11 ± 3 | 14 ± 7 |
SRPE | PRS | |||
---|---|---|---|---|
Pre | Post | Pre | Post | |
EIMD AX (n = 10) PLA (n = 9) | 8.6 ± 0.8 7.4 ± 1.0 | 8.3 ± 0.7 7.8 ± 0.7 | 8.6 ± 0.8 9.0 ± 1.1 | 8.4 ± 1.5 8.5 ± 1.9 |
PT AX (n = 10) PLA (n = 9) | 7.3 ± 0.9 7.3 ± 0.9 | 7.8 ± 1.1 7.0 ± 1.1 | 6.8 ± 2.0 6.6 ± 1.8 | 7.7 ± 0.8 6.7 ± 1.9 |
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Barker, G.A.; Parten, A.L.; Lara, D.A.; Hannon, K.E.; McAllister, M.J.; Waldman, H.S. Astaxanthin Supplementation Reduces Subjective Markers of Muscle Soreness following Eccentric Exercise in Resistance-Trained Men. Muscles 2023, 2, 228-237. https://doi.org/10.3390/muscles2020017
Barker GA, Parten AL, Lara DA, Hannon KE, McAllister MJ, Waldman HS. Astaxanthin Supplementation Reduces Subjective Markers of Muscle Soreness following Eccentric Exercise in Resistance-Trained Men. Muscles. 2023; 2(2):228-237. https://doi.org/10.3390/muscles2020017
Chicago/Turabian StyleBarker, Gaven A., Alyssa L. Parten, David A. Lara, Kensey E. Hannon, Matthew J. McAllister, and Hunter S. Waldman. 2023. "Astaxanthin Supplementation Reduces Subjective Markers of Muscle Soreness following Eccentric Exercise in Resistance-Trained Men" Muscles 2, no. 2: 228-237. https://doi.org/10.3390/muscles2020017