Physicochemical and Microstructural Analyses of Pepsin-Soluble Collagens Derived from Lizardfish (Saurida tumbil Bloch, 1795) Skin, Bone and Scales
Abstract
:1. Introduction
2. Results and Discussion
2.1. Yield and Hydroxyproline Composition
2.2. Color Attributes
2.3. SDS-PAGE Analysis
2.4. UV-Vis Absorption
2.5. Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy (ATR–FTIR)
2.6. Evaluation of X-ray Diffraction (XRD)
2.7. Thermostability Analysis
2.8. Microstructure Study
2.9. Solubility
3. Conclusions
4. Materials and Method
4.1. Preparation of Lizardfish Skin, Bone and Scales
4.2. Extraction of Pepsin-Soluble Collagen
4.3. Analyses of Pepsin-Soluble Collagen
4.3.1. Measurement of Yield and Hyp Content
4.3.2. Color Measurement
4.3.3. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
4.3.4. UV-Vis Spectra
4.3.5. Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy (ATR–FTIR)
4.3.6. Determination of X-ray Diffraction (XRD)
4.3.7. Determination of Differential Scanning Calorimetry (DSC)
4.3.8. Field Emission Scanning Electron Microscopy (FESEM)
4.3.9. Solubility Measurement
4.4. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Yield (%) Wet Weight | Hyp (mg/g) | Collagen (mg/g) | Color Parameters | |||
---|---|---|---|---|---|---|---|
L* | a* | b* | WI | ||||
PSSC | 3.50 ± 0.11 a | 85.71 ± 0.13 a | 659.94 ± 0.99 a | 64.87 ± 3.19 b | 3.46 ± 0.38 a | 6.83 ± 0.73 b | 64.03 ± 3.24 b |
PSBC | 3.26 ± 0.10 a | 84.85 ± 1.38 a | 653.33 ± 10.60 a | 79.91 ± 0.04 a | 0.31 ± 0.35 b | 3.59 ± 0.06 c | 79.58 ± 0.03 a |
PSCC | 0.60 ± 0.06 b | 81.72 ± 0.47 b | 629.28 ± 3.61 b | 81.04 ± 0.45 a | 0.17 ± 0.13 b | 11.95 ± 1.34 a | 77.57 ± 0.97 a |
Peak Type | Peak Description | Peak Location | ||
---|---|---|---|---|
PSSC | PSBC | PSCC | ||
Amide A | N-H stretching coupled with hydrogen bond | 3278.28 cm−1 | 3295.05 cm−1 | 3293.19 cm−1 |
Amide B | CH2 asymmetric stretching | 2922.31 cm−1 | 2922.31 cm−1 | 2922.31 cm−1 |
Amide I | C=O stretching/hydrogen bond coupled with COO- | 1636.34 cm−1 | 1636.34 cm−1 | 1636.34 cm−1 |
Amide II | N-H bend coupled with C-N stretching | 1541.29 cm−1 | 1541.29 cm−1 | 1541.29 cm−1 |
Amide III | N-H bend coupled with C-H stretching | 1233.78 cm−1 | 1233.78 cm−1 | 1237.51 cm−1 |
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Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Physicochemical and Microstructural Analyses of Pepsin-Soluble Collagens Derived from Lizardfish (Saurida tumbil Bloch, 1795) Skin, Bone and Scales. Gels 2022, 8, 471. https://doi.org/10.3390/gels8080471
Jaziri AA, Shapawi R, Mokhtar RAM, Noordin WNM, Huda N. Physicochemical and Microstructural Analyses of Pepsin-Soluble Collagens Derived from Lizardfish (Saurida tumbil Bloch, 1795) Skin, Bone and Scales. Gels. 2022; 8(8):471. https://doi.org/10.3390/gels8080471
Chicago/Turabian StyleJaziri, Abdul Aziz, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, and Nurul Huda. 2022. "Physicochemical and Microstructural Analyses of Pepsin-Soluble Collagens Derived from Lizardfish (Saurida tumbil Bloch, 1795) Skin, Bone and Scales" Gels 8, no. 8: 471. https://doi.org/10.3390/gels8080471