Preparation, Physicochemical Characterization, Antimicrobial Effects, Biocompatibility and Cytotoxicity of Co-Loaded Meropenem and Vancomycin in Mesoporous Silica Nanoparticles
Abstract
:1. Introduction
2. Material and Methods
2.1. Preparation of Vancomycin/Meropenem-Loaded MSNPs
2.2. Characterization
2.2.1. Fourier-Transform Infrared Spectroscopy (FTIR)
2.2.2. Dynamic Light Scattering (DLS)
2.2.3. Scanning Electron Microscope (SEM)
2.3. Drug Loading and the Drug Release Pattern
2.4. Drug Release Pattern
2.5. Bacterial Isolates
2.6. Antibacterial Effects
2.7. Antibiofilm Property
2.8. The Effect of Van/Mrp-MSNPs on the Microbial Attachment
2.9. Van/Mrp-MSNPs Effects on RBCs
2.10. Interaction of Van/Mrp-MSNPs and Human Plasma Protein
2.11. Cytotoxic Effects of Van/Mrp-MSNPs In Vitro
2.12. Statistical Analysis
3. Results
3.1. Antibacterial Effects
3.2. Antibiofilm Effects
3.3. Hemolytic Effects and RBCs Sedimentation upon Exposure to Van/Mrp-MSNPs
3.4. Interaction of Van/Mrp-MSNPs and Human Plasma Protein
3.5. Cytotoxic Effects of Van/Mrp-MSNPs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate Number | MIC of Vancomycin (µg/mL) | MIC of Meropenem (µg/mL) | FICI | ||
---|---|---|---|---|---|
Alone | In Combination | Alone | In Combination | ||
SA1 | 2 | 0.5 | 128 | 32 | 0.5 |
SA2 | 1 | 0.25 | 64 | 8 | 0.37 |
SA3 | 1 | 0.5 | 64 | 8 | 0.62 |
SA4 | 2 | 0.5 | 128 | 32 | 0.5 |
SA5 | 0.5 | 0.125 | 128 | 32 | 0.5 |
ATCC 43300 | 1 | 0.125 | 64 | 16 | 0.37 |
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Yekani, M.; Azargun, R.; Sharifi, S.; Sadri Nahand, J.; Hasani, A.; Ghanbari, H.; Sadat Seyyedi, Z.; Memar, M.Y.; Maleki Dizaj, S. Preparation, Physicochemical Characterization, Antimicrobial Effects, Biocompatibility and Cytotoxicity of Co-Loaded Meropenem and Vancomycin in Mesoporous Silica Nanoparticles. Biomedicines 2023, 11, 3075. https://doi.org/10.3390/biomedicines11113075
Yekani M, Azargun R, Sharifi S, Sadri Nahand J, Hasani A, Ghanbari H, Sadat Seyyedi Z, Memar MY, Maleki Dizaj S. Preparation, Physicochemical Characterization, Antimicrobial Effects, Biocompatibility and Cytotoxicity of Co-Loaded Meropenem and Vancomycin in Mesoporous Silica Nanoparticles. Biomedicines. 2023; 11(11):3075. https://doi.org/10.3390/biomedicines11113075
Chicago/Turabian StyleYekani, Mina, Robab Azargun, Simin Sharifi, Javid Sadri Nahand, Alka Hasani, Hadi Ghanbari, Zahra Sadat Seyyedi, Mohammad Yousef Memar, and Solmaz Maleki Dizaj. 2023. "Preparation, Physicochemical Characterization, Antimicrobial Effects, Biocompatibility and Cytotoxicity of Co-Loaded Meropenem and Vancomycin in Mesoporous Silica Nanoparticles" Biomedicines 11, no. 11: 3075. https://doi.org/10.3390/biomedicines11113075