The Micromorphology and Histochemistry of Foliar Mixed Indumentum of Leucas lavandulaefolia (Lamiaceae)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material Collection
2.2. Scanning Electron Microscopy
2.3. Light and Transmission Electron Microscopy
2.4. Histochemistry
3. Results and Discussion
3.1. Microscopic Characterization of Foliar Secretory Structures
3.2. Histochemistry
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Leaf Developmental Stage | |||
---|---|---|---|
Trichome Type | Emergent | Young | Mature |
Peltate | 20.78 ± 3.89 µm | 30.66 ± 3.00 µm | 42.80 ± 2.82 µm |
Short-stalked | 14.98 ± 3.29 µm | 17.12 ± 0.74 µm | 18.93 ± 4.96 µm |
Target Constituents | Test | Peltate Trichomes | Capitate Trichomes | Non-Glandular Trichomes | Colour Reaction | |||
---|---|---|---|---|---|---|---|---|
Head Cells | Subcuticular Space | Head Cells | Subcuticular Space | Cellular Pedestal | Basal Cells | |||
Essential oils | NADI reagent | ++ | ++ | + | + | − | − | Positive—blue |
Acidic lipids | Nile blue A | − | − | − | − | − | − | Positive—blue |
Alkaloids | Wagner and Dittmar | − | − | − | − | − | − | Positive—orange/brown |
Phenolic compounds | Ferric trichloride | + | − | + | + | − | − | Positive—black deposits |
Mucilage and pectins | Ruthenium red | − | − | − | − | − | Positive—pink/red | |
Lipids | Sudan Black | − | − | − | − | ++ | ++ | Positive—blue-black |
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Naidoo, Y.; Dladla, T.; Dewir, Y.H.; Gangaram, S.; Naidoo, C.M.; Rihan, H.Z. The Micromorphology and Histochemistry of Foliar Mixed Indumentum of Leucas lavandulaefolia (Lamiaceae). Plants 2021, 10, 1767. https://doi.org/10.3390/plants10091767
Naidoo Y, Dladla T, Dewir YH, Gangaram S, Naidoo CM, Rihan HZ. The Micromorphology and Histochemistry of Foliar Mixed Indumentum of Leucas lavandulaefolia (Lamiaceae). Plants. 2021; 10(9):1767. https://doi.org/10.3390/plants10091767
Chicago/Turabian StyleNaidoo, Yougasphree, Thobekile Dladla, Yaser Hassan Dewir, Serisha Gangaram, Clarissa Marcelle Naidoo, and Hail Z. Rihan. 2021. "The Micromorphology and Histochemistry of Foliar Mixed Indumentum of Leucas lavandulaefolia (Lamiaceae)" Plants 10, no. 9: 1767. https://doi.org/10.3390/plants10091767