Gold Leaf-Based Microfluidic Platform for Detection of Essential Oils Using Impedance Spectroscopy
Abstract
:1. Introduction
2. Experimental
2.1. Materials and Apparatus
2.2. Fabrication of Microfluidic Device
2.3. Electrochemical Measurements and EO Sample Preparation
3. Results and Discussion
3.1. Characterization of Au Leaf
3.2. EIS Studies
3.3. Calculation of Root Mean Square Deviation (RMSD) as a Tool to Differentiate Impact of Essential Oil Type and Concentration
4. Conclusions and Future Prospects
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | Value |
Cutter plotter | |
Cutting speed | 3 cm/s |
Cutting force PVC 80 µm | 19 (out of 38 steps from 0.2 to 4.41 N) |
Cutting force PVC 125 µm | 26 (out of 38 steps from 0.2 to 4.41 N) |
Hot lamination | |
Temperature | 160 °C |
Speed | 3 cm/min |
EOs in the Channel | Z’ [Ω] at 10 kHz 1% | Z’ [Ω] at 10 kHz 5% |
---|---|---|
Peppermint | 1.26 | 1.07 |
Eucalyptus | 1.79 | 1.45 |
EOs in the Channel | Time | RMSD 1% | RMSD 5% |
---|---|---|---|
Peppermint | 0 h | 4.29 | 2.39 |
Peppermint | After 22 h | 7.73 | 8.57 |
Peppermint | After 46 h | 21.62 | 12.33 |
Eucalyptus | 0 h | 18.12 | 3.61 |
Eucalyptus | After 22 h | 23.07 | 6.64 |
Eucalyptus | After 46 h | 47.88 | 30.65 |
Substrate | Electrode Material | Method of Fabrication | Target | Reference |
---|---|---|---|---|
Polydimethoxy silane | Ti | Lithography | HEK29, HCT116 cell lines | [38] |
Indium tin oxide-coated glass | Au | Photolithography | HeLa, NIH-3T3, and CHO-K cell culture | [39] |
Glass | Au | Dielectrophoresis | E. coli | [40] |
Glass | Au | Sputtering | Tomato Ringspot virus | [41] |
Indium tin oxide-coated glass | Au | Photolithography | Human periph-eral blood mononuclear cell | [42] |
Polyvinyl chloride | Ag | Xurography | EOs | [29] |
Polyvinyl chloride | Au | Xurography | EOs | This work |
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Sinha, A.; Stavrakis, A.K.; Simić, M.; Kojić, S.; Stojanović, G.M. Gold Leaf-Based Microfluidic Platform for Detection of Essential Oils Using Impedance Spectroscopy. Biosensors 2022, 12, 1169. https://doi.org/10.3390/bios12121169
Sinha A, Stavrakis AK, Simić M, Kojić S, Stojanović GM. Gold Leaf-Based Microfluidic Platform for Detection of Essential Oils Using Impedance Spectroscopy. Biosensors. 2022; 12(12):1169. https://doi.org/10.3390/bios12121169
Chicago/Turabian StyleSinha, Ankita, Adrian K. Stavrakis, Mitar Simić, Sanja Kojić, and Goran M. Stojanović. 2022. "Gold Leaf-Based Microfluidic Platform for Detection of Essential Oils Using Impedance Spectroscopy" Biosensors 12, no. 12: 1169. https://doi.org/10.3390/bios12121169