Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Instruments
2.2. Preparation of Anti-PEDV Polyclonal Antibody
2.3. Preparation of the Anti-PEDV Antibodies Modified Capture Chip
2.4. Preparation of Specific GNR Probes
2.5. Sandwich Immunoassay on the Capture Chip
2.6. GNR Probe-Assisted DFM Counting Strategy for Detection of PEDV Samples
2.7. Sensitivity of GNR Probe-Assisted DFM Counting Strategy
2.8. Preparation of Simulated Real Samples for DFM Counting
3. Results and Discussion
3.1. Specificity of Anti-PEDV Polyclonal Antibody
3.2. Characterization and Optimization of GNR Probes
3.3. Specificity of GNR Probes
3.4. Characterization of the Capture Chips
3.5. GNR Probe-Assisted DFM Counting of PEDV in PBS
3.6. GNR Probe-Assisted DFM Counting of PEDV in Simulated Real Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Qian, X.; Shen, Y.; Yuan, J.; Yang, C.-T.; Zhou, X. Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field. Biosensors 2022, 12, 1146. https://doi.org/10.3390/bios12121146
Qian X, Shen Y, Yuan J, Yang C-T, Zhou X. Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field. Biosensors. 2022; 12(12):1146. https://doi.org/10.3390/bios12121146
Chicago/Turabian StyleQian, Xuejia, Yuanzhao Shen, Jiasheng Yuan, Chih-Tsung Yang, and Xin Zhou. 2022. "Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field" Biosensors 12, no. 12: 1146. https://doi.org/10.3390/bios12121146