Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Virus, Cells, and S. suis Strains
2.3. Construction of Recombinant BoHV-4/GMD and BoHV-4/SLY BACs Expressing S. suis Antigens
2.4. Genomic Characterization of BoHV-4 BACs
2.5. Reconstitution of Recombinant BoHV-4/GMD and BoHV-4/SLY Viruses
2.6. Western Immunoblot Analysis of S. suis Antigen Expression
2.7. Preparation of Recombinant BoHV-4 Virus Stocks
2.8. Rabbit Immunization and S. suis Challenge
2.9. ELISA for Detection of S. suis Antibodies
2.10. ELISA for Detection of IL-4 and IFN-γ
2.11. Antibody-Mediated Phagocytic Activity against S. suis
2.12. Statistical Analysis
3. Results
3.1. Construction of BoHV-4/GMD and BoHV-4/SLY BACs Expressing S. suis Antigens
3.2. Reconstitution and Characterization of BoHV-4/GMD and BoHV-4/SLY
3.3. Antibody Responses in Rabbits Immunized with BoHV-4/GMD via Different Administration Routes
3.4. BoHV-4/GMD and BoHV-4/SLY Induce Antibody Responses in Rabbits against SS2, SS7, and SS9
3.5. Levels of Cytokines in Rabbits Immunized with BoHV-4/GMD and BoHV-4/SLY
3.6. Protective Efficacy of BoHV-4/GMD and BoHV-4/SLY against SS2 Challenge in Rabbits
3.7. Antibody-Mediated Phagocytic Activity against SS2, SS7, and SS9 in Immunized Rabbits
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dong, N.; Nichols, H.; Sun, Q.; Chen, X.; Zheng, J.; Guan, Z.; Zhang, H.; Davison, A.; Wezel, Y.; Li, Z.; et al. Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model. Vaccines 2023, 11, 1004. https://doi.org/10.3390/vaccines11051004
Dong N, Nichols H, Sun Q, Chen X, Zheng J, Guan Z, Zhang H, Davison A, Wezel Y, Li Z, et al. Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model. Vaccines. 2023; 11(5):1004. https://doi.org/10.3390/vaccines11051004
Chicago/Turabian StyleDong, Nihua, Hester Nichols, Qing Sun, Xiaojun Chen, Jiayang Zheng, Zhixin Guan, Hailong Zhang, Andrew Davison, Yvonne Wezel, Zongjie Li, and et al. 2023. "Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model" Vaccines 11, no. 5: 1004. https://doi.org/10.3390/vaccines11051004