Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Ethics Statement
2.2. Cell Culture and Treatment
2.3. Hematoxylin–Eosin (H&E)
2.4. Protein Extraction and Western Blot Analysis
2.5. ROS Level Determination
2.6. Transmission Electron Microscopy
2.7. Mitochondrial Membrane Potential and Apoptosis Detection
2.8. Detection of Intracellular Adenosine Triphosphate (ATP)
2.9. Cell Viability Assay
2.10. Detection of Cytoplasmic Ca2+
2.11. Statistical Analysis
3. Results
3.1. Apoptosis-Related Protein Caspase-3, Cyto-C, and mPTP Key Protein CypD Were Significantly Increased in Dairy Cow Uteri with Endometritis
3.2. Mitochondrial Dysfunction Promotes the Expression of Apoptotic Proteins of BEECs
3.3. ROS Removal by NAC Prevented Mitochondrial Vacuolization in BEECs
3.4. ROS Removal by NAC Restores Mitochondrial Membrane Potential and Energy Synthesis to Normal Levels
3.5. ROS Removal by NAC Treatment Significantly Reduced the Expression of Mitochondria-Dependent Apoptotic Proteins
3.6. mPTP Inhibition Prevented the Release of Cyto-C and Ca 2+ into the Cytoplasm
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Song, P.; Sun, M.; Liu, C.; Liu, J.; Lin, P.; Chen, H.; Zhou, D.; Tang, K.; Wang, A.; Jin, Y. Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway. Antioxidants 2023, 12, 2123. https://doi.org/10.3390/antiox12122123
Song P, Sun M, Liu C, Liu J, Lin P, Chen H, Zhou D, Tang K, Wang A, Jin Y. Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway. Antioxidants. 2023; 12(12):2123. https://doi.org/10.3390/antiox12122123
Chicago/Turabian StyleSong, Pengjie, Mingkun Sun, Chen Liu, Jianguo Liu, Pengfei Lin, Huatao Chen, Dong Zhou, Keqiong Tang, Aihua Wang, and Yaping Jin. 2023. "Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway" Antioxidants 12, no. 12: 2123. https://doi.org/10.3390/antiox12122123