Protective Effect of the Hydrophilic Extract of Polypodium leucotomos, Fernblock®, against the Synergistic Action of UVA Radiation and Benzo[a]pyrene Pollutant
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. UVA Light Irradiation
2.3. Combined Treatment with BaP (+/−FB) and UVA Light Exposure
2.4. Cell Viability Assessment
2.5. Reactive Oxygen Species Determination
2.6. Indirect Immunofluorescence
2.7. Mitochondrial Morphology
2.8. Quantification of the Expression of Opsin-3 by Real Time Polymerase Chain Reaction
2.9. Statistical Analyses
3. Results
3.1. Characterization of the Photoprotective Effects of FB on Cell Viability and Morphology
3.2. Evaluation of Oxidative Stress Levels
3.3. Mitochondrial Morphology and Localization of Cytochrome C
3.4. Evaluation of DNA Damage
3.5. Effect on the Expression of Opsin-3 in B16-F10 Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gallego-Rentero, M.; Nicolás-Morala, J.; Alonso-Juarranz, M.; Carrasco, E.; Portillo-Esnaola, M.; Rodríguez-Luna, A.; González, S. Protective Effect of the Hydrophilic Extract of Polypodium leucotomos, Fernblock®, against the Synergistic Action of UVA Radiation and Benzo[a]pyrene Pollutant. Antioxidants 2022, 11, 2185. https://doi.org/10.3390/antiox11112185
Gallego-Rentero M, Nicolás-Morala J, Alonso-Juarranz M, Carrasco E, Portillo-Esnaola M, Rodríguez-Luna A, González S. Protective Effect of the Hydrophilic Extract of Polypodium leucotomos, Fernblock®, against the Synergistic Action of UVA Radiation and Benzo[a]pyrene Pollutant. Antioxidants. 2022; 11(11):2185. https://doi.org/10.3390/antiox11112185
Chicago/Turabian StyleGallego-Rentero, María, Jimena Nicolás-Morala, Miguel Alonso-Juarranz, Elisa Carrasco, Mikel Portillo-Esnaola, Azahara Rodríguez-Luna, and Salvador González. 2022. "Protective Effect of the Hydrophilic Extract of Polypodium leucotomos, Fernblock®, against the Synergistic Action of UVA Radiation and Benzo[a]pyrene Pollutant" Antioxidants 11, no. 11: 2185. https://doi.org/10.3390/antiox11112185