Ethylene Induced by Sound Stimulation Enhances Anthocyanin Accumulation in Grape Berry Skin through Direct Upregulation of UDP-Glucose: Flavonoid 3-O-Glucosyltransferase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Sound Stimulation of VR Cells
2.3. Sound Stimulation of Bunches on Field-Grown Grapevine
2.4. Measurement of Total Anthocyanin
2.5. Berry Characteristics
2.6. RNA Isolation
2.7. Real-Time RT-PCR Analysis
2.8. Ethephon Treatment of VR Cells
2.9. Ethylene Biosynthesis Inhibitor Treatment of VR Cells Exposed to Sound Stimulation
2.10. Assay of UFGT Promoter in Response to Ethylene
2.11. Statistical Analysis
3. Results
3.1. Sound Stimulation Enhances Anthocyanin Accumulation in VR Cells
3.2. Sound Stimulation Accelerates Anthocyanin Accumulation in Berry Skins of Field-Grown Grapevines
3.3. Effect of Sound Stimulation on MybA1 and UFGT Transcription
3.4. Effect of Sound Stimulation on ABA and Ethylene Biosynthesis
3.5. Activation of UFGT Promoter by Ethephon
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yamazaki, M.; Ishida, A.; Suzuki, Y.; Aoki, Y.; Suzuki, S.; Enoki, S. Ethylene Induced by Sound Stimulation Enhances Anthocyanin Accumulation in Grape Berry Skin through Direct Upregulation of UDP-Glucose: Flavonoid 3-O-Glucosyltransferase. Cells 2021, 10, 2799. https://doi.org/10.3390/cells10102799
Yamazaki M, Ishida A, Suzuki Y, Aoki Y, Suzuki S, Enoki S. Ethylene Induced by Sound Stimulation Enhances Anthocyanin Accumulation in Grape Berry Skin through Direct Upregulation of UDP-Glucose: Flavonoid 3-O-Glucosyltransferase. Cells. 2021; 10(10):2799. https://doi.org/10.3390/cells10102799
Chicago/Turabian StyleYamazaki, Mone, Akari Ishida, Yutaka Suzuki, Yoshinao Aoki, Shunji Suzuki, and Shinichi Enoki. 2021. "Ethylene Induced by Sound Stimulation Enhances Anthocyanin Accumulation in Grape Berry Skin through Direct Upregulation of UDP-Glucose: Flavonoid 3-O-Glucosyltransferase" Cells 10, no. 10: 2799. https://doi.org/10.3390/cells10102799