Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chitosan
- C1, HCl standard solution concentration (mol/L);
- C2, NaOH standard solution concentration (mol/L);
- V1, drop the HCl standard solution volume (mL);
- V2, consume NaOH standard solution volume (mL);
- G, sample weight (g);
- W, sample moisture content (%);
- 0.016, amount of amine equivalent to 1 mL of 1 mol/L HCl (g);
- 0.094, theoretical amine group content in chitosan.
2.2. Preparation of Chitosan Lactate Particles (CLP)
2.3. Particles Morphology, Size and Zeta Potential Analysis
2.4. Cytotoxicity
- Asample: absorbance values of the cells treated with the sample extract.
- Acontrol: absorbance values after treatment of cells with basal medium.
2.5. Hemolysis Evaluation
- Asample: absorbance values after sample treatment.
- Anegative: absorbance values after normal saline treatment.
- Apositive: absorbance values after distilled water treatment.
2.6. Antimicrobial Test
- NC: the number of bacterial colonies in the control group.
- NE: the number of bacterial colonies surviving in the experiment group.
2.7. Liquid Absorption
- Wwet: the wet weight of the sample.
- Wdry: the dry weight of the sample.
2.8. In Vitro Blood Coagulation Test
2.9. Red Blood Cells (RBCs) Adhesion Test
- ODhemostat: the OD540 nm value after the hemostat treatment.
- ODreference: the OD540 nm value without the hemostatic agent treatment.
2.10. Platelets Adhesion Test
- ODhemostat: the OD490 nm value after the hemostat treatment.
- ODreference: the OD490 nm value without the hemostatic agent treatment.
2.11. Hemostatic Test In Vivo
2.12. Statistics and Reproducibility
3. Results and Discussions
3.1. Preparation and Characterization
3.2. Biocompatibility
3.3. Anti-Infection Properties In Vitro
3.4. Capacity of Liquid/Blood Absorption
3.5. In Vitro Procoagulability
3.6. Hemostatic Effects of CLP In Vivo
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jiang, Y.; Tang, X.; Li, T.; Ling, J.; Ge, Y.; Yang, Y. Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection. Polymers 2023, 15, 656. https://doi.org/10.3390/polym15030656
Jiang Y, Tang X, Li T, Ling J, Ge Y, Yang Y. Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection. Polymers. 2023; 15(3):656. https://doi.org/10.3390/polym15030656
Chicago/Turabian StyleJiang, Yuhui, Xiaoxuan Tang, Tao Li, Jue Ling, Yifan Ge, and Yumin Yang. 2023. "Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection" Polymers 15, no. 3: 656. https://doi.org/10.3390/polym15030656