Maternal n-7 Unsaturated Fatty Acids Protect the Fetal Brain from Neuronal Degeneration in an Intrauterine Hyperglycemic Animal Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cell Culture
2.3. Cell Viability Assay
2.4. Immunoblotting Assay
2.5. Detection of Ubiquitinated Proteins
2.6. Measurement of Intracellular Reactive Oxygen Species (ROS) Levels
2.7. Annexin V/Propidium Iodide (PI) Double Staining Assay
2.8. Statistical Analysis
3. Results
3.1. Effect of a Hyperglycemic Environment and Functional Lipids during Pregnancy in GDM Rat Models
3.1.1. Body Weights and Blood Glucose Levels of GDM Rat Pups
3.1.2. AGEs, RAGE, and Insulin Signaling in GDM rat pups
3.2. Effects of Unsaturated Fatty Acids on PC12 Cells Exposed to High-Glucose Medium
3.2.1. AGEs, RAGE, and Insulin Signaling in PC12 Cells
3.2.2. Apoptosis Signaling in PC12 Cells
3.2.3. ROS Production and Apoptosis Induction in PC12 Cells
3.3. Effects of Unsaturated Fatty Acids in Differentiated PC12 Cells Exposed to High-glucose medium
3.3.1. AGEs, RAGE, and Insulin Signaling in Differentiated PC12 Cells
3.3.2. Apoptosis Signaling in Differentiated PC12 Cells
3.3.3. ROS Production and Apoptosis Induction in Differentiated PC12 Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | PCM (n = 10) | PDM (n = 10) | PDM/CPA (n = 10) | PDM/TPA (n = 10) |
---|---|---|---|---|
Body weight (mean ± SE, g) | 6.0 ± 0.4 | 6.1 ± 0.9 | 5.1 ± 0.5 | 6.2 ± 0.4 |
Blood glucose level (mean ± SE, mg/dL) | 70.1 ± 13.0 | 72.3 ± 13.1 | 77.7 ± 25.0 | 70.0 ± 10.7 |
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Okami, H.; Kawaharada, R.; Yoshizaki, H.; Toriumi, A.; Tsutsumi, S.; Nakamura, A. Maternal n-7 Unsaturated Fatty Acids Protect the Fetal Brain from Neuronal Degeneration in an Intrauterine Hyperglycemic Animal Model. Nutrients 2023, 15, 3434. https://doi.org/10.3390/nu15153434
Okami H, Kawaharada R, Yoshizaki H, Toriumi A, Tsutsumi S, Nakamura A. Maternal n-7 Unsaturated Fatty Acids Protect the Fetal Brain from Neuronal Degeneration in an Intrauterine Hyperglycemic Animal Model. Nutrients. 2023; 15(15):3434. https://doi.org/10.3390/nu15153434
Chicago/Turabian StyleOkami, Haruka, Ritsuko Kawaharada, Hitomi Yoshizaki, Akiyo Toriumi, Saki Tsutsumi, and Akio Nakamura. 2023. "Maternal n-7 Unsaturated Fatty Acids Protect the Fetal Brain from Neuronal Degeneration in an Intrauterine Hyperglycemic Animal Model" Nutrients 15, no. 15: 3434. https://doi.org/10.3390/nu15153434