Topic Editors

Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing, China
School of Biological Science & Medical Engineering, Southeast University, Nanjing, China

Optical Molecular Sensing and Imaging: Development and Applications

Abstract submission deadline
20 May 2024
Manuscript submission deadline
20 July 2024
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1349

Topic Information

Dear Colleagues,

Determining molecular properties and functions is important for deeply understanding cell activities and developing novel therapeutic strategies. The optical sensing and imaging approaches are powerful tools for studying molecular events owing to the noninvasive and dynamic monitoring features. Label-free sensing approaches have shown distinct advantages in analyzing intrinsic molecular properties, such as mass, diffusion, and binding kinetics. Recently developed label-free single-molecule imaging approaches, including interferometric scattering microscopy, plasmonic scattering microscopy, and evanescent scattering microscopy, have further advanced this field by pushing beyond the ensemble average to reveal the statistical distributions of molecular properties in both pure buffer and complex biological media at the single molecular level. At the same time, the NIR fluorescence imaging approaches have gained significant attention in molecular imaging due to their deep tissue penetration, reduced scattering, and autofluorescence background. In this issue, we focus on the new development of highly sensitive label-free biosensing and imaging approaches and the NIR biosensing, and especially the integrated imaging approaches combining the label-free and NIR biosensors to reveal the multiple properties of biological processes. We invite research submissions capable of helping advance the field of optical molecular imaging and their applications for the efficient analysis of biological activities.

Dr. Pengfei Zhang
Dr. Rui Wang
Topic Editors

Keywords

  • label-free biosensors
  • optical molecular imaging
  • surface plasmon resonance
  • evanescent scattering
  • cell imaging
  • near-infrared imaging
  • in vivo biosensing

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Biosensors
biosensors
5.4 4.9 2011 17.4 Days CHF 2700 Submit
Sensors
sensors
3.9 6.8 2001 17 Days CHF 2600 Submit

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Published Papers (1 paper)

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14 pages, 2762 KiB  
Article
Photonic Crystal Surface Mode Real-Time Imaging of RAD51 DNA Repair Protein Interaction with the ssDNA Substrate
Biosensors 2024, 14(1), 43; https://doi.org/10.3390/bios14010043 - 14 Jan 2024
Viewed by 967
Abstract
Photonic crystals (PCs) are promising tools for label-free sensing in drug discovery screening, diagnostics, and analysis of ligand–receptor interactions. Imaging of PC surface modes has emerged as a novel approach to the detection of multiple binding events at the sensor surface. PC surface [...] Read more.
Photonic crystals (PCs) are promising tools for label-free sensing in drug discovery screening, diagnostics, and analysis of ligand–receptor interactions. Imaging of PC surface modes has emerged as a novel approach to the detection of multiple binding events at the sensor surface. PC surface modification and decoration with recognition units yield an interface providing the highly sensitive detection of cancer biomarkers, antibodies, and oligonucleotides. The RAD51 protein plays a central role in DNA repair via the homologous recombination pathway. This recombinase is essential for the genome stability and its overexpression is often correlated with aggressive cancer. RAD51 is therefore a potential target in the therapeutic strategy for cancer. Here, we report the designing of a PC-based array sensor for real-time monitoring of oligonucleotide–RAD51 recruitment by means of surface mode imaging and validation of the concept of this approach. Our data demonstrate that the designed biosensor ensures the highly sensitive multiplexed analysis of association–dissociation events and detection of the biomarker of DNA damage using a microfluidic PC array. The obtained results highlight the potential of the developed technique for testing the functionality of candidate drugs, discovering new molecular targets and drug entities. This paves the way to further adaption and bioanalytical use of the biosensor for high-content screening to identify new DNA repair inhibitor drugs targeting the RAD51 nucleoprotein filament or to discover new molecular targets. Full article
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