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Water
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Geomicrobiology in Extreme Environments
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Geomicrobiology in Extreme Environments

A special issue of Water (ISSN 2073-4441). This special issue belongs to the section "Water Quality and Contamination".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 1260

Special Issue Editors

Dr. Geng Wu

E-Mail Website
Guest Editor
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China
Interests: hot spring; mineral; C; N; S; biogeochemistry cycling; arsenic
Prof. Dr. Yun Fang

E-Mail Website
Guest Editor
School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China
Interests: industrial microorganisms; biotechnology and engineering; biochemical engineering; fermentation engineering; applied microorganisms
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Geomicrobes are widely distributed in various extreme environments, such as acid mine drainage (AMD), hot spring, reservoir, and deep subsurface. Functional extremophiles are very powerful forces that shape our planet’s environment.

We sincerely invite contributions to this Special Issue on aspects listed in the following keywords, covering recent advances and innovations in extreme environments, which will be of interest to researchers and practitioners in the geomicrobiology field. The topics of interest include but are not limited to:

  • Acidophiles in AMD;
  • Halophilic archaea in saline lakes;
  • Deep-sea hydrothermal vents;
  • Virus in extreme environments;
  • Sulfur-reduction microorganisms in hot springs;
  • Hydrocarbon degradation bacteria in subsurface biosphere;
  • Groundwater microorganisms in a Karst environment.

Dr. Geng Wu
Prof. Dr. Yun Fang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Water is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • geomicrobiology
  • C/N/S cycling
  • arsenic
  • extremophile
  • Fe(II)/Fe(III)
  • mineral

Published Papers (1 paper)

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Research

14 pages, 3901 KiB  
Article
Characterization of a Thermophilic and Inhibitor-Tolerant GH1 β-Glucosidase Present in a Hot Spring
by Yu-Ying Huang, Pei Wu, Xing-Ci Wu, Qian-Ru Zhu, Qian Zhu, Hong-Zhao Zheng, Dan Zhu, Zhi-Hua Lv and Yi-Rui Yin
Water 2023, 15(19), 3389; https://doi.org/10.3390/w15193389 - 27 Sep 2023
Cited by 1 | Viewed by 995
Abstract
β-glucosidase is a key enzyme in the degradation of lignocellulosic biomass, which is responsible for the conversion of oligosaccharides from cellulose hydrolysates to glucose. However, its required high temperatures and the presence of inhibitors have limited its use in industry. In this study, [...] Read more.
β-glucosidase is a key enzyme in the degradation of lignocellulosic biomass, which is responsible for the conversion of oligosaccharides from cellulose hydrolysates to glucose. However, its required high temperatures and the presence of inhibitors have limited its use in industry. In this study, a new β-glucosidase gene, named thbg2, was obtained from the metagenome Ruidian Hot Spring, Tengchong City, Yunnan Province, southwestern China. The gene was synthesized, cloned, heterologously expressed, and enzymatically characterized. Its optimum temperature and pH were 60 °C and pH 5.6, respectively. ThBg2 exhibited more than 60% relative activity in temperatures ranging from 40 °C to 70 °C and across a pH of 4.0–6.6. It maintained 100% relative activity after incubation at either 50 °C for 24 h or 60 °C for 12 h and more than 80% residual activity after incubation at pH 4.0–6.0 for 24 h. Moreover, it maintained more than 80% relative activity in the presence of heavy metal ions, ethanol, SDS etc. Furthermore, glucose yields from corn stalks increased by 20% after ThBg2 (0.05 mg/mL) was added to the commercial cellulase reaction system. Overall, this work identified a thermophilic and inhibitor-tolerant β-glucosidase with potential applications in commercial lignocellulose utilization and the bioenergy industry. Full article
(This article belongs to the Special Issue Geomicrobiology in Extreme Environments)
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