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Vaccines
Special Issues
Gene-Based Vaccines
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Gene-Based Vaccines

A special issue of Vaccines (ISSN 2076-393X). This special issue belongs to the section "DNA and mRNA Vaccines".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 8619

Special Issue Editor

Dr. Michael A. Barry

E-Mail Website
Guest Editor
Department of Medicine, Mayo Clinic, Rochester, MN 55902, USA
Interests: gene-based vaccines; gene therapy; oncolytic virotherapy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The COVID-19 pandemic has brought gene-based vaccines into the forefront of human medicine, whether they be mRNA vaccines or DNA-based vaccines. This issue will examine the biology, application, immunology, and practical aspects of using gene-based vaccines for many pathogens, including SARS-CoV-2. 

Dr. Michael A. Barry
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Vaccines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • gene-based vaccines
  • mRNA vaccines
  • viral vectors
  • non-viral vectors
  • lipid nanoparticles
  • electroporation
  • mucosal vaccines
  • systemic vaccines

Published Papers (1 paper)

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Research

35 pages, 7788 KiB  
Article
Biophysical Characterization of Viral and Lipid-Based Vectors for Vaccines and Therapeutics with Light Scattering and Calorimetric Techniques
by Natalia Markova, Stefan Cairns, Hanna Jankevics-Jones, Michael Kaszuba, Fanny Caputo and Jérémie Parot
Vaccines 2022, 10(1), 49; https://doi.org/10.3390/vaccines10010049 - 30 Dec 2021
Cited by 9 | Viewed by 8045
Abstract
Novel vaccine platforms for delivery of nucleic acids based on viral and non-viral vectors, such as recombinant adeno associated viruses (rAAV) and lipid-based nanoparticles (LNPs), hold great promise. However, they pose significant manufacturing and analytical challenges due to their intrinsic structural complexity. During [...] Read more.
Novel vaccine platforms for delivery of nucleic acids based on viral and non-viral vectors, such as recombinant adeno associated viruses (rAAV) and lipid-based nanoparticles (LNPs), hold great promise. However, they pose significant manufacturing and analytical challenges due to their intrinsic structural complexity. During product development and process control, their design, characterization, and quality control require the combination of fit-for-purpose complementary analytical tools. Moreover, an in-depth methodological expertise and holistic approach to data analysis are required for robust measurements and to enable an adequate interpretation of experimental findings. Here the combination of complementary label-free biophysical techniques, including dynamic light scattering (DLS), multiangle-DLS (MADLS), Electrophoretic Light Scattering (ELS), nanoparticle tracking analysis (NTA), multiple detection SEC and differential scanning calorimetry (DSC), have been successfully used for the characterization of physical and chemical attributes of rAAV and LNPs encapsulating mRNA. Methods’ performance, applicability, dynamic range of detection and method optimization are discussed for the measurements of multiple critical physical−chemical quality attributes, including particle size distribution, aggregation propensity, polydispersity, particle concentration, particle structural properties and nucleic acid payload. Full article
(This article belongs to the Special Issue Gene-Based Vaccines)
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