Current Aspects of Listeriosis

A special issue of Tropical Medicine and Infectious Disease (ISSN 2414-6366). This special issue belongs to the section "Infectious Diseases".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 2856

Special Issue Editors


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Guest Editor
1. Clinical Unit of Infectious Diseases, Microbiology and Parasitology, Hospital Universitario Virgen del Rocio, 41013 Sevilla, Spain
2. CSIC-JA-USE - Instituto de Biomedicina de Sevilla, 41013 Sevilla, Spain
Interests: listeriosis; antibiotic management of invasive infection; study of antibiotic resistance mechanisms; antibiotic stewardship; rapid diagnostic methods for invasive infection; molecular epidemiology methods for infection management

E-Mail Website
Guest Editor
1. Clinical Unit of Infectious Diseases, Microbiology and Parasitology, Hospital Universitario Virgen del Rocio, 41013 Sevilla, Spain
2. CSIC-JA-USE—Instituto de Biomedicina de Sevilla, 41013 Sevilla, Spain
Interests: antimicrobial resistance; listeria monocytogenes; outbreaks; molecular epidemiology; nosocomial infections

Special Issue Information

Dear Colleagues,

Listeria monocytogenes is an intracellular bacteria that causes an invasive disease in humans, listeriosis, a zoonosis with well-defined risk groups: over 65 years of age, immunocompromised, pregnant women, and neonates. This microorganism has the exceptional ability to cross biological barriers: intestinal, haemato-encephalic, and placental, generally causing abortion, septicemia, or central nervous system infections, with a mortality rate of around 30%.

Listeriosis generally occurs in sporadic cases; however, new molecular typing techniques have revealed the existence of previously unsuspected epidemic outbreaks.

The importance of listeriosis is not sufficiently recognized as it is a relatively uncommon disease. In fact, it is considered a rare disease included in ORPHANET, the European Consortium for Orphan Diseases and Orphan Medicinal Products under the reference ORPHA533.

Listeriosis is a food-borne disease in adults, but in newborns, it is a congenital disease in most cases. It is also one of the few congenital infections where antibiotic treatment can improve clinical outcomes.

Furthermore, listeriosis is a significant problem for the food industry, and many countries have introduced legislation to control the incidence of the disease.

Dr. José Antonio Lepe
Dr. Ángel Rodríguez-Villodres
Guest Editors

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Keywords

  • listeriosis
  • food-borne disease
  • food industry
  • infection
  • Listeria monocytogenes

Published Papers (1 paper)

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Research

9 pages, 1730 KiB  
Article
In Vitro and In Vivo Virulence Study of Listeria monocytogenes Isolated from the Andalusian Outbreak in 2019
by Andrea Vila Domínguez, Marta Carretero Ledesma, Carmen Infante Domínguez, José Miguel Cisneros, Jose A. Lepe and Younes Smani
Trop. Med. Infect. Dis. 2023, 8(1), 58; https://doi.org/10.3390/tropicalmed8010058 - 12 Jan 2023
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Abstract
In 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging to clonal complex 388 (Lm CC388), has been isolated. We aimed to [...] Read more.
In 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging to clonal complex 388 (Lm CC388), has been isolated. We aimed to determine the Lm CC388 virulence in comparison with other highly virulent clones such as Lm CC1 and Lm CC4, in vitro and in vivo. Four L. monocytogenes strains (Lm CC388, Lm CC1, Lm CC4 and ATCC 19115) were used. Attachment to human lung epithelial cells (A549 cells) by these strains was characterized by adherence and invasion assays. Their cytotoxicities to A549 cells were evaluated by determining the cells viability. Their hemolysis activity was determined also. A murine intravenous infection model using these was performed to determine the concentration of bacteria in tissues and blood. Lm CC388 interaction with A549 cells is non-significantly higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Lm CC388 cytotoxicity is higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Moreover, Lm CC388 hemolysis activity is lower than that of the Lm CC4 strain, and higher than that of Lm CC1. Finally, in the murine intravenous infection model by Lm CC388, higher bacterial loads in tissues and at similar levels of Lm CC4 were observed. Although a lower rate of mortality of patients during the listeriosis outbreak in Spain in 2019 has been reported, the Lm CC388 strain has shown a greater or similar pathogenicity level in vitro and in an animal model, like Lm CC1 and Lm CC4. Full article
(This article belongs to the Special Issue Current Aspects of Listeriosis)
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