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Separations
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Advanced Methods for Separation and Analysis of Natural Products
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Advanced Methods for Separation and Analysis of Natural Products

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 2588

Special Issue Editor

Dr. Chua Lee Suan

E-Mail Website
Guest Editor
Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Malaysia
Interests: herbal and bioproduct processing

Special Issue Information

Dear Colleagues,

Natural products, including herbs, are incredibly complex in terms of their chemical composition. Herbs are unique and can usually be found in specific locations. They are mostly applied by Indigenous people as traditional remedies for overall well-being. Their wide application is mainly due to the presence of many naturally occurring plant constituents that are beneficial to human health. The benefits could be further value-added through the application of processing technology to separate/concentrate bioactive compounds and/or to remove undesirable substances. Possibly, value-added products will be processed in a convenient format for better delivery and assessment. To ensure the quality of the products or herbal extracts, it is of great importance to apply technically reliable analytical methods for quality assurance. Therefore, this Special Issue is introduced to gather any advanced technologies from worldwide researchers with the aim to enhance the values of local natural products.

Dr. Chua Lee Suan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • herbal extraction
  • herbal drying
  • herbal profiling
  • herbal authentication
  • bioproducts formulation

Published Papers (2 papers)

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Research

11 pages, 2888 KiB  
Article
A Green and Cost-Effective Chromatographic Method for the Measurement of the Activity of Human Serum Carnosinase
by Chiara de Giacomi and Luca Regazzoni
Separations 2023, 10(8), 460; https://doi.org/10.3390/separations10080460 - 21 Aug 2023
Viewed by 794
Abstract
Carnosinase (i.e., CN1; E.C. 3.4.13.20) is an enzyme found in the sera of higher primates. CN1 preferentially catalyzes the hydrolysis of natural, orally adsorbed histidine dipeptides like carnosine (i.e., β-alanyl-L-histidine). This is the reason why carnosine has a limited use as a human [...] Read more.
Carnosinase (i.e., CN1; E.C. 3.4.13.20) is an enzyme found in the sera of higher primates. CN1 preferentially catalyzes the hydrolysis of natural, orally adsorbed histidine dipeptides like carnosine (i.e., β-alanyl-L-histidine). This is the reason why carnosine has a limited use as a human food supplement or pharmacological agent, despite the promising results obtained in experiments on animal models of human diseases. Herein, an assay is reported for the measurement of serum CN1 activity. The method is intended for the screening of CN1 inhibitors able to enhance carnosine bioavailability in humans. The method was developed to monitor serum hydrolytic activity via the quantitation of one of the products of carnosine hydrolysis (i.e., histidine). Separation was achieved without using organic solvents by means of ion chromatography (IC), whereas detection was provided by UV spectroscopy. The assay herein reported is a green and cost-effective alternative to a recently published method based on hydrophilic interaction liquid chromatography (HILIC) and mass spectrometry (MS). The results show that such a method produces reliable measurements of serum hydrolytic activity and can be used for the screening of CN1 inhibitors. Full article
(This article belongs to the Special Issue Advanced Methods for Separation and Analysis of Natural Products)
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11 pages, 1058 KiB  
Article
Identification and Quantification of Polyphenolic Secondary Metabolites in Stem Bark of Ficus religiosa (Moraceae) Using UPLC-HRMS and RP-HPLC-PDA
by Deepak Thakur, Uma Ranjan Lal, Deepak N. Kapoor and Deepak Kumar
Separations 2023, 10(6), 338; https://doi.org/10.3390/separations10060338 - 31 May 2023
Cited by 3 | Viewed by 1388
Abstract
F. religiosa bark has been extensively used in traditional medicinal systems, such as Ayurveda, for its health benefits. The aim of this study was to investigate the secondary metabolites (phenolics and flavonoids) of the hydroalcoholic stem-bark extract from F. religiosa because this plant [...] Read more.
F. religiosa bark has been extensively used in traditional medicinal systems, such as Ayurveda, for its health benefits. The aim of this study was to investigate the secondary metabolites (phenolics and flavonoids) of the hydroalcoholic stem-bark extract from F. religiosa because this plant has been proven to have a beneficial effect on health disorders. Therefore, a pilot study was conducted for the identification and quantification of polyphenolic compounds in F. religiosa bark using sophisticated chromatographical techniques such as UPLC-HRMS and RP-HPLC-PDA. Additionally, total flavonoids, total phenolics and the scavenging profile of the bark were studied using a UV spectrophotometer. A total of 23 compounds identified with UPLC-HRMS were mainly phenolic acids, polyphenolics, and flavonoids (flavanols and proanthocyanidins). Among the identified compounds, gallic acid, catechin, epicatechin, epigallocatechin gallate, and ellagic acid were simultaneously quantified (0.031–0.380%) using RP-HPLC-PDA. Thereafter, the study complied by evaluating the total flavonoids (109.15 ± 1.2 mg RuE/g and 33.78 ± 0.86 mg CaE/g), total phenolics (4.81 ± 1.01 mg GaE/g), and scavenging profiles (IC50 13.75 ± 0.12 µg/mL) of the F. religiosa bark. This is the first report on the chemical profiling of F. religiosa bark, which is a necessary step to evaluate its nutraceutical properties, paving the way for possible food application. Full article
(This article belongs to the Special Issue Advanced Methods for Separation and Analysis of Natural Products)
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