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Organoids
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Organoids and Advanced 3D Models in Biomedical Research
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Organoids and Advanced 3D Models in Biomedical Research

A special issue of Organoids (ISSN 2674-1172).

Deadline for manuscript submissions: closed (25 January 2024) | Viewed by 2005

Special Issue Editor

Dr. Marta Alves da Silva

E-Mail Website
Guest Editor
Laboratory Animal Science Group, i3S-Instituto de Investigação e Inovação em Saúde, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
Interests: organoids; stem cells; advanced 3D models; microfluidics; disease modelling; regenerative medicine; tissue engineering
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are pleased to invite you to submit your paper to our special issue on Organoids and Advanced 3D in vitro models.

Biomedical research faces a demanding need for the replacement of animal experiments by reliable, safe, and accurate in vitro models that fully recapitulate the structure and function of human tissues. Advances in stem cells bioengineering enabled adult stem cells (ASCs) and induced pluripotent stem cells (iPSCs) long-term culture in a 3D cellular structure named organoids. They recreate cellular architecture; are functionally similar to the tissue they are modeling and their use as models allow research without confusing influences from the local microenvironment.

This Special Issue aims to disseminate state-of-the-art science around organoid model systems, covering organoids models in 3D microenvironments, genetic engineering of organoids and microphysiologic systems, as well as methods for improving organoids culture and high throughput screening.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but not limited to) the following: disease modeling, personalized medicine, drug screening, tissue engineering, biomaterials, regenerative medicine and 3Rs.

I look forward to receiving your contributions.

You may choose our Joint Special Issue in Biomolecules.

Dr. Marta Alves da Silva
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Organoids is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • organoids
  • in vitro models
  • microenvironment
  • regenerative medicine
  • non-animal models
  • microfluidics

Published Papers (1 paper)

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Research

18 pages, 4192 KiB  
Article
A Microwell Device for the Efficient Generation of Arrays of Microtissues and Humanized Bone Marrow Micro-Ossicles
by Kathryn Futrega, Md. Shafiullah Shajib, Pamela G. Robey and Michael R. Doran
Organoids 2023, 2(2), 102-119; https://doi.org/10.3390/organoids2020008 - 01 Jun 2023
Viewed by 1479
Abstract
(1) Background: There are no high-throughput microtissue platforms for generating bone marrow micro-ossicles. Herein, we describe a method for the assembly of arrays of microtissues from bone marrow stromal cells (BMSC) in vitro and their maturation into bone marrow micro-ossicles in vivo. (2) [...] Read more.
(1) Background: There are no high-throughput microtissue platforms for generating bone marrow micro-ossicles. Herein, we describe a method for the assembly of arrays of microtissues from bone marrow stromal cells (BMSC) in vitro and their maturation into bone marrow micro-ossicles in vivo. (2) Methods: Discs with arrays of 50 microwells were used to assemble microtissues from 3 × 105 BMSCs each on a nylon mesh carrier. Microtissues were cultured in chondrogenic induction medium followed by hypertrophic medium in an attempt to drive endochondral ossification, and then they were implanted in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice, where they were remodeled into bone marrow micro-ossicles. Mice were transplanted with 105 human umbilical cord blood CD34+ cells. (3) Results: Micro-ossicles contained more human CD45+ cells, but fewer human CD34+ progenitor cells than mouse marrow. Human hematopoietic progenitor cells cycle rapidly at non-physiological rates in mouse marrow, and reduced CD34+ cell content in micro-ossicles is consistent with the notion that the humanized niche better controls progenitor cell cycling. (4) Conclusions: Assembling microtissues in microwells, linked by a nylon membrane carrier, provides an elegant method to manufacture and handle arrays of microtissues with bone organ-like properties. More generally, this approach and platform could aid bridging the gap between in vitro microtissue manipulation and in vivo microtissue implantation. Full article
(This article belongs to the Special Issue Organoids and Advanced 3D Models in Biomedical Research)
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