Bioprinting and Nano-Biomaterials in Tissue Engineering and Regenerative Medicine

A special issue of Nanomaterials (ISSN 2079-4991). This special issue belongs to the section "Biology and Medicines".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 3420

Special Issue Editor


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Guest Editor
Institute of Polymers, Composites and Biomaterials, National Research Council of Italy, Naples, Italy
Interests: design for additive manufacturing; reverse engineering; 3D/4D printing; bioprinting; design methods; creative design; design of experiments; mechanical analysis; modeling and simulation; lattice structures; lightweight structures; biomechanics; biomimetics and bioinspiration; prosthesis design; scaffold design for tissue engineering; design of drug delivery systems; computer-aided molecular design; dental materials; nanomaterials; polymers and composites

Special Issue Information

Dear Colleagues,

The combination of 3D bioprinting technologies and nano-biomaterials may open a wide range of perspectives towards the generation of highly complex cell-laden constructs in the field of tissue engineering and regenerative medicine. The possibility to overcome critical weaknesses of obtained products, at the same time improving their structural and functional features, will allow the success of existing studies, especially in the perspective of in vitro and in vivo applications. Cell behavior over time may benefit from natural mimicking microenvironment, specific biochemical and biophysical cues, as well as from a specific array of bioactive stimuli able to modulate cell fate. Morphological, topographical, and chemical features of 3D bioprinted structures, through the employment of micro- or nano-structured bioactive materials, may be—directly or indirectly—involved in tissue regeneration processes.

Smart, instructive cell-free or cell-laden structures able to tune and guide tissue reconstruction represents the greatest challenge in tissue engineering and regenerative medicine.

More recently, the possibility has emerged to create active structures in which sensing, motion, and shape or functionalities can be modified with time by applying external stimuli. These responsive structures could broaden applications in 4D bioprinting for regenerative medicine and drug delivery applications.

The present Special Issue would attract ideas regarding contributions from leading scientists and research groups in the specific field, with the aim of giving a balanced view of the current state-of-the-art and perspectives. Review articles, as well as original research, could contribute to summarize the state of the art and to provide recent advances or novel guidelines and tools in improving the knowledge on this intriguing topic.

Dr. Russo Teresa
Guest Editor

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Keywords

  • 3D/4D printing
  • bioprinting
  • design for additive manufacturing
  • biomechanics
  • biomimetics
  • bioinspiration
  • gels/hydrogels
  • bioactive nanomaterials

Published Papers (1 paper)

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Research

22 pages, 4296 KiB  
Article
Alginate-Lysozyme Nanofibers Hydrogels with Improved Rheological Behavior, Printability and Biological Properties for 3D Bioprinting Applications
by Maria C. Teixeira, Nicole S. Lameirinhas, João P. F. Carvalho, Bruno F. A. Valente, Jorge Luís, Liliana Pires, Helena Oliveira, Martinho Oliveira, Armando J. D. Silvestre, Carla Vilela and Carmen S. R. Freire
Nanomaterials 2022, 12(13), 2190; https://doi.org/10.3390/nano12132190 - 26 Jun 2022
Cited by 11 | Viewed by 2989
Abstract
In this study, alginate nanocomposite hydrogel bioinks reinforced with lysozyme nanofibers (LNFs) were developed. Alginate-LNF (A-LNF) suspensions with different LNF contents (1, 5 and 10 wt.%) were prepared and pre-crosslinked with 0.5% (w/v) CaCl2 to formulate A-LNF inks. [...] Read more.
In this study, alginate nanocomposite hydrogel bioinks reinforced with lysozyme nanofibers (LNFs) were developed. Alginate-LNF (A-LNF) suspensions with different LNF contents (1, 5 and 10 wt.%) were prepared and pre-crosslinked with 0.5% (w/v) CaCl2 to formulate A-LNF inks. These inks exhibit proper shear-thinning behavior and good recovery properties (~90%), with the pre-crosslinking step playing a crucial role. A-LNF fully crosslinked hydrogels (with 2% (w/v) CaCl2) that mimic 3D printing scaffolds were prepared, and it was observed that the addition of LNFs improved several properties of the hydrogels, such as the morphology, swelling and degradation profiles, and mechanical properties. All formulations are also noncytotoxic towards HaCaT cells. The printing parameters and 3D scaffold model were then optimized, with A-LNF inks showing improved printability. Selected A-LNF inks (A-LNF0 and A-LNF5) were loaded with HaCaT cells (cell density 2 × 106 cells mL−1), and the cell viability within the bioprinted scaffolds was evaluated for 1, 3 and 7 days, with scaffolds printed with the A-LNF5 bioink showing the highest values for 7 days (87.99 ± 1.28%). Hence, A-LNF bioinks exhibited improved rheological performance, printability and biological properties representing a good strategy to overcome the main limitations of alginate-based bioinks. Full article
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