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Modern Isolation Techniques in the Recovery of Bioactive Natural Products from Plant Matrices

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: closed (31 January 2023) | Viewed by 4185

Special Issue Editors


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Guest Editor
National and Kapodistrian University of Athens, Athens, Greece
Interests: phytochemicals; bioactivity antioxidant activity; chromatography extraction; food chemistry; mass spectrometry; antioxidants; natural product chemistry; antibacterial activity

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Guest Editor
Department of Pharmacognosy and Natural Product Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimioupoli, Zografou, GR-15771 Athens, Greece
Interests: green chemistry and large-scale extraction of high added-value natural products; isolation of natural products leads; identification and structural elucidation of natural products; semi- and total synthesis of natural products; characterization of natural extracts; design and synthesis of new molecules with pharmacological interest; structure–activity relationships
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Special Issue Information

Dear Colleagues,

Plant extracts are rich sources of natural products of differing chemical characteristics and structures. Many of them have been commonly used in the traditional medicine around the world to heal various types of ailments. Still, these days primary and secondary metabolites of plant origin have a function of drugs or scaffoldings for the semi-synthesis of new, often more active, pharmaceuticals.

That is why the necessity to perform isolation and identification studies of the extracts’ constituents is of the highest importance. Single molecules with confirmed bioactivity can be better validated in terms of their toxicity, active doses, mechanisms of action, interactions with other drugs, or stability. The recent development of analytical equipment with high measurement accuracy and selectivity provides better chances for the isolation of natural products. On the other hand, a wide range of in vitro and in vivo assays are able to trace promising compounds―drug candidates of different kind―in the total extracts.

This Special Issue will present interdisciplinary manuscripts covering the application of modern extraction and isolation techniques in the recovery of pharmacologically potent metabolites.

Prof. Wirginia Kukula-Koch
Dr. Apostolis Angelis
Prof. Alexios Leandros Skaltsounis
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • extraction and isolation
  • plant extracts
  • metabolites
  • natural products
  • identification and structure elucidation

Published Papers (2 papers)

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Research

24 pages, 1661 KiB  
Article
The Influence of Microwave-Assisted Extraction on the Phenolic Compound Profile and Biological Activities of Extracts from Selected Scutellaria Species
by Joanna Oracz, Stanisław Kowalski, Dorota Żyżelewicz, Gabriela Kowalska, Dorota Gumul, Kamila Kulbat-Warycha, Justyna Rosicka-Kaczmarek, Anna Brzozowska, Aleksandra Grzegorczyk and Anna Areczuk
Molecules 2023, 28(9), 3877; https://doi.org/10.3390/molecules28093877 - 4 May 2023
Cited by 1 | Viewed by 1406
Abstract
The aim of the study was to investigate the effects of microwave-assisted extraction (MAE) conditions (microwave power, extraction time, and ethanol concentration) on the efficiency of the extraction of phenolic compounds from selected plant species belonging to the genus Scutellaria (i.e., Scutellaria baicalensis [...] Read more.
The aim of the study was to investigate the effects of microwave-assisted extraction (MAE) conditions (microwave power, extraction time, and ethanol concentration) on the efficiency of the extraction of phenolic compounds from selected plant species belonging to the genus Scutellaria (i.e., Scutellaria baicalensis and Scutellaria lateriflora). The extracts from selected Scutellaria species were examined to establish the total phenolic content and the in vitro antioxidant and anti-inflammatory activity. The antioxidant capacity was determined by the ferric reducing antioxidant power (FRAP) and 2,2,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity methods. The anti-inflammatory activity was evaluated through the lipoxygenase (LOX) inhibitory assay. The phenolic profile of the extracts was characterized using ultra-high performance liquid chromatography coupled with diode array detection and high-resolution electrospray ionization mass spectrometry (UHPLC–DAD/ESI–HRMS/MS). Depending on the type of solvent and the extraction conditions used, the extracts obtained from selected Scutellaria species showed different total and individual phenolic content, as well as different antioxidant and anti-inflammatory properties. The results showed that all Scutellaria extracts had high total phenolic content and exhibited strong ferric ion reducing power and free radical scavenging capacity and a significant ability to inhibit the LOX activity. In general, the 70% ethanol extracts contained more phenolic compounds, mainly flavones, flavanones, and their derivatives, and showed greater in vitro biological activity than other extracts. The highest levels of phenolic compounds and the strongest antioxidant and anti-inflammatory potential were found in extracts from the roots of S. baicalensis. Optimal extraction conditions for all the plant materials tested were determined as the microwave power of 63 W, extraction time of 10 min, and 70% ethanol as the solvent. Full article
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23 pages, 9031 KiB  
Article
Ultrasound-Assisted Extraction of Antioxidants from Melastoma malabathricum Linn.: Modeling and Optimization Using Box–Behnken Design
by Suzziyana Hosni, Siti Salwa Abd Gani, Valérie Orsat, Masriana Hassan and Sumaiyah Abdullah
Molecules 2023, 28(2), 487; https://doi.org/10.3390/molecules28020487 - 4 Jan 2023
Cited by 5 | Viewed by 2032
Abstract
This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extraction temperature (XET), ultrasonic time (X [...] Read more.
This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extraction temperature (XET), ultrasonic time (XUT), solvent concentration (XSC), and sample-to-liquid ratio (XSLR). Response surface methodology (RSM) coupled with Box–Behnken design (BBD) was applied to establish optimum conditions for maximum antioxidant extraction. Modeling and optimization conditions of UAE at 37 kHz, XET 32 °C for XUT 16 min and dissolved in an XSC 70% ethanol concentration at a XSLR 1:10 ratio yielded scavenging effects on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) at 96% ± 1.48 and recorded values of total phenolic content (TPC) and total flavonoid content (TFC) at 803.456 ± 32.48 mg GAE (gallic acid equivalents)/g, and 102.972 ± 2.51 mg QE (quercetin equivalents)/g, respectively. The presence of high flavonoid compounds was verified using TWIMS-QTOFMS. Chromatic evaluation of phytochemicals using gas chromatography–mass spectrometry (GC–MS) revealed the presence of 14 phytocompounds widely documented to play significant roles in human health. This study provides a comparative evaluation with other studies and may be used for validation of the species’ potential for its much-acclaimed medicinal and cosmeceutical uses. Full article
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