Micro/Nanofluidics Devices for Nucleic Acids and Cell Analysis

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology and Biomedicine".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 7656

Special Issue Editors


E-Mail Website
Guest Editor
State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China
Interests: electrochemical biosensors; microfluidic platforms; microelectrode arrays; nerve signal detection; point-of-care testing
Mechanical Engineering, University of Minnesota, Twin Cities, 111 Church Street SE, Minneapolis, MN 55455, USA
Interests: nucleic acid analysis; biomedical MEMS devices; microfluidic devices; electrochemical biosensors; point-of-care testing

E-Mail Website
Guest Editor
College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Interests: microfluidic platforms; electrokinetics; high-throughput single cell sequencing and analysis; conducting elastomer based sensing

Special Issue Information

Dear Colleagues,

Micro/nanofluidics technology, having the advantages of precise fluid control and minimal reagent use, has received great attention for a wide variety of applications, including diagnosis, electronics, nanofabrication and cell-based screening. Furthermore, the integration of micro/nanofluidics with nucleic acid or cell analysis leads to novel analysis devices, advancing new research hotspots. The combination of micro/nanofluidics devices with nucleic acid has been used to improve the diagnosis of numerous infectious diseases, early cancer screening and treatment assessment. Moreover, microelectrode arrays integrated with microfluidics have important application prospects in exploring the mechanisms of neurological diseases and the fields of drug screening, neural computing and organ chips.

This Special Issue, “Micro/Nanofluidics Devices for Nucleic Acids and Cell Analysis”, seeks to showcase research papers, short communications and review articles focusing on the effective integration of micro/nanofluidic devices and nucleic acid testing methods. Particular attention will be paid to innovative applications that can improve upon existing medical devices and brain–machine interfaces. Also of interest is the development of micro/nano fluidic devices for nucleic analysis, which presents a great challenge as many steps, including cell or virus lysis, nucleic acid extraction and enrichment and nucleic acid amplification or detection signal amplification, must be accomplished by a sensitive, portable yet low-cost chip.

As the field of micro/nanofluidics devices for nucleic acids and cell analysis has a wide scope and touches upon many application areas, it is often difficult to locate a single source of relevant information.

Dr. Jinping Luo
Dr. Yang Wang
Dr. Xiaoxing Xing
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Dr. Jinping Luo
Dr. Yang Wang
Dr. Xiaoxing Xing
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • gene sequencing
  • DNA storage
  • nucleic acid amplification detection
  • methylation analysis
  • single nucleotide polymorphism
  • microRNA detection
  • spatial transcriptome sequencing
  • digital nucleic acid amplification
  • CRISPR-based detection nucleic acid molecule detection
  • point-of-care devices
  • micro-electrode array
  • biomedical MEMS/NEMS devices
  • paper-based devices
  • microfluidic/nanofluidic devices
  • microdroplets

Published Papers (4 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

11 pages, 4450 KiB  
Article
A Portable, Integrated, Sample-In Result-Out Nucleic Acid Diagnostic Device for Rapid and Sensitive Chikungunya Virus Detection
by Changping Xu, Yalin Chen, Guiying Zhu, Huan Wu, Qi Jiang, Rui Zhang, Beibei Yu, Lei Fang and Zhiwei Wu
Micromachines 2024, 15(5), 663; https://doi.org/10.3390/mi15050663 - 19 May 2024
Viewed by 494
Abstract
Chikungunya virus, a mosquito-borne virus that causes epidemics, is often misdiagnosed due to symptom similarities with other arboviruses. Here, a portable and integrated nucleic acid-based diagnostic device, which combines reverse transcription-loop-mediated isothermal amplification and lateral-flow detection, was developed. The device is simple to [...] Read more.
Chikungunya virus, a mosquito-borne virus that causes epidemics, is often misdiagnosed due to symptom similarities with other arboviruses. Here, a portable and integrated nucleic acid-based diagnostic device, which combines reverse transcription-loop-mediated isothermal amplification and lateral-flow detection, was developed. The device is simple to use, precise, equipment-free, and highly sensitive, enabling rapid chikungunya virus identification. The result can be obtained by the naked eye within 40 min. The assay can effectively distinguish chikungunya virus from dengue virus, Japanese encephalitis virus, Zika virus, and yellow fever virus with high specificity and sensitivity as low as 598.46 copies mL−1. It has many benefits for the community screening and monitoring of chikungunya virus in resource-limited areas because of its effectiveness and simplicity. The platform has great potential for the rapid nucleic acid detection of other viruses. Full article
(This article belongs to the Special Issue Micro/Nanofluidics Devices for Nucleic Acids and Cell Analysis)
Show Figures

Figure 1

10 pages, 1225 KiB  
Article
Rapid Detection of Malaria Based on Hairpin-Mediated Amplification and Lateral Flow Detection
by Yang Zhang, Lihui Ke, Tao Sun, Yang Liu, Bo Wei and Minghua Du
Micromachines 2023, 14(10), 1917; https://doi.org/10.3390/mi14101917 - 9 Oct 2023
Viewed by 1043
Abstract
Malaria is listed as one of the three most hazardous infectious diseases worldwide. Travelers and migrants passing through exit and entry ports are important sources of malaria pandemics globally. Developing accurate and rapid detection technology for malaria is important. Here, a novel hairpin-mediated [...] Read more.
Malaria is listed as one of the three most hazardous infectious diseases worldwide. Travelers and migrants passing through exit and entry ports are important sources of malaria pandemics globally. Developing accurate and rapid detection technology for malaria is important. Here, a novel hairpin-mediated amplification (HMA) technique was proposed for the detection of four Plasmodium species, including P. falciparum, P. vivax, P. malariae, and P. ovale. Based on the conserved nucleotide sequence of Plasmodium, specific primers and probes were designed for the HMA process, and the amplicon can be detected using lateral flow detection (LFD); the results can be read visually without specialized equipment. The specificity of HMA-LFD was evaluated using nucleic acids extracted from four different Plasmodium species and two virus species. The sensitivity of HMA-LFD was valued using 10× serial dilutions of plasmid containing the template sequence. Moreover, 78 blood samples were collected to compare HMA-LFD and qPCR. The HMA-LFD results were all positive for four different Plasmodium species and negative for the other two virus species. The sensitivity of HMA-LFD was tested to be near five copies/μL. The analysis of clinical samples indicated that the consistency of HMA-LFD and qPCR was approximately 96.15%. Based on these results, the HMA-LFD assay was demonstrated to be a rapid, sensitive, and specific technique for the detection of Plasmodium and has great advantages for on-site detection in low-resource areas and exit and entry ports. Full article
(This article belongs to the Special Issue Micro/Nanofluidics Devices for Nucleic Acids and Cell Analysis)
Show Figures

Figure 1

13 pages, 5630 KiB  
Article
Design of a Digital LAMP Detection Platform Based on Droplet Microfluidic Technology
by Liying Jiang, Xianghao Lan, Linjiao Ren, Mingzhu Yang, Bo Wei and Yang Wang
Micromachines 2023, 14(5), 1077; https://doi.org/10.3390/mi14051077 - 19 May 2023
Cited by 1 | Viewed by 1642
Abstract
Loop-mediated isothermal amplification (LAMP) is a rapid and high-yield amplification technology for specific DNA or RNA molecules. In this study, we designed a digital loop-mediated isothermal amplification (digital-LAMP)-functioning microfluidic chip to achieve higher sensitivity for detection of nucleic acids. The chip could generate [...] Read more.
Loop-mediated isothermal amplification (LAMP) is a rapid and high-yield amplification technology for specific DNA or RNA molecules. In this study, we designed a digital loop-mediated isothermal amplification (digital-LAMP)-functioning microfluidic chip to achieve higher sensitivity for detection of nucleic acids. The chip could generate droplets and collect them, based on which we could perform Digital-LAMP. The reaction only took 40 min at a constant temperature of 63 °C. The chip enabled highly accurate quantitative detection, with the limit of detection (LOD) down to 102 copies μL−1. For better performance while reducing the investment of money and time in chip structure iterations, we used COMSOL Multiphysics to simulate different droplet generation ways by including flow-focusing structure and T-junction structure. Moreover, the linear structure, serpentine structure, and spiral structure in the microfluidic chip were compared to study the fluid velocity and pressure distribution. The simulations provided a basis for chip structure design while facilitating chip structure optimization. The digital-LAMP-functioning chip proposed in the work provides a universal platform for analysis of viruses. Full article
(This article belongs to the Special Issue Micro/Nanofluidics Devices for Nucleic Acids and Cell Analysis)
Show Figures

Figure 1

Review

Jump to: Research

25 pages, 5770 KiB  
Review
Recent Progress and Perspectives on Neural Chip Platforms Integrating PDMS-Based Microfluidic Devices and Microelectrode Arrays
by Shihong Xu, Yaoyao Liu, Yan Yang, Kui Zhang, Wei Liang, Zhaojie Xu, Yirong Wu, Jinping Luo, Chengyu Zhuang and Xinxia Cai
Micromachines 2023, 14(4), 709; https://doi.org/10.3390/mi14040709 - 23 Mar 2023
Cited by 3 | Viewed by 3908
Abstract
Recent years have witnessed a spurt of progress in the application of the encoding and decoding of neural activities to drug screening, diseases diagnosis, and brain–computer interactions. To overcome the constraints of the complexity of the brain and the ethical considerations of in [...] Read more.
Recent years have witnessed a spurt of progress in the application of the encoding and decoding of neural activities to drug screening, diseases diagnosis, and brain–computer interactions. To overcome the constraints of the complexity of the brain and the ethical considerations of in vivo research, neural chip platforms integrating microfluidic devices and microelectrode arrays have been raised, which can not only customize growth paths for neurons in vitro but also monitor and modulate the specialized neural networks grown on chips. Therefore, this article reviews the developmental history of chip platforms integrating microfluidic devices and microelectrode arrays. First, we review the design and application of advanced microelectrode arrays and microfluidic devices. After, we introduce the fabrication process of neural chip platforms. Finally, we highlight the recent progress on this type of chip platform as a research tool in the field of brain science and neuroscience, focusing on neuropharmacology, neurological diseases, and simplified brain models. This is a detailed and comprehensive review of neural chip platforms. This work aims to fulfill the following three goals: (1) summarize the latest design patterns and fabrication schemes of such platforms, providing a reference for the development of other new platforms; (2) generalize several important applications of chip platforms in the field of neurology, which will attract the attention of scientists in the field; and (3) propose the developmental direction of neural chip platforms integrating microfluidic devices and microelectrode arrays. Full article
(This article belongs to the Special Issue Micro/Nanofluidics Devices for Nucleic Acids and Cell Analysis)
Show Figures

Figure 1

Back to TopTop