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Micromachines
Special Issues
Microfluidics Technologies for Cell-Based Assays, Volume II
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Microfluidics Technologies for Cell-Based Assays, Volume II

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B：Biology and Biomedicine".

Deadline for manuscript submissions: closed (30 September 2021) | Viewed by 2291

Special Issue Editors

Prof. Dr. Khashayar Khoshmanesh

E-Mail Website
Guest Editor
School of Engineering, RMIT University, City Campus, Melbourne, VIC 3001, Australia
Interests: microfluidics; lab-on-a-chip; organ-on-a-chip; mechanobiology; soft matter
Special Issues, Collections and Topics in MDPI journals
Dr. Sara Baratchi

E-Mail Website
Guest Editor
School of Health and Biomedical Sciences, RMIT University, Bundoora Campus, Melbourne, VIC 3083, Australia
Interests: mechanobiology; bio-microfluidics; cell biology; atherosclerosis; super-resolution microscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microfluidic systems are increasingly used for conducting cell-based assays. Such systems enable monitoring cellular responses under well-controlled physical (mechanical, shear stress, thermal, optical) and chemical (drugs, chemicals, nanomaterials) stimuli to mimic various physiological and pathological cues, allowing for more realistic in vitro models. Furthermore, advancement of microfabrication technologies has facilitated highly integrated and multifunctional organ-on-a-chip systems that can replace the time-consuming and expensive ex vivo and in vivo models. This Special Issue seeks to showcase research papers, short communications, and review articles reporting the latest developments in this exciting and multidisciplinary field. The topics include but are not limited to the following: (i) studying the viability, proliferation, metabolism, signaling, migration, and morphology of cells; (ii) sorting and patterning of cells; and (iii) development of disease-on-a-chip and organ-on-a-chip models using microfluidic technologies.

Assoc. Prof. Khashayar Khoshmanesh
Dr. Sara Baratchi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Microfluidics
  • Lab-on-a-chip
  • Cellular assays
  • Cell stimulation
  • Mechanobiology
  • Disease-on-a-chip
  • Organ-on-a-chip

Published Papers (1 paper)

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Research

15 pages, 3016 KiB  
Article
A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers
by Jia Hao, Winfield Zhao, Jeong Min Oh and Keyue Shen
Micromachines 2021, 12(10), 1254; https://doi.org/10.3390/mi12101254 - 16 Oct 2021
Viewed by 1856
Abstract
Chemotactic cell migration plays a crucial role in physiological and pathophysiological processes. In tissues, cells can migrate not only through extracellular matrix (ECM), but also along stromal cell surfaces via membrane-bound receptor–ligand interactions to fulfill critical functions. However, there remains a lack of [...] Read more.
Chemotactic cell migration plays a crucial role in physiological and pathophysiological processes. In tissues, cells can migrate not only through extracellular matrix (ECM), but also along stromal cell surfaces via membrane-bound receptor–ligand interactions to fulfill critical functions. However, there remains a lack of models recapitulating chemotactic migration mediated through membrane-bound interactions. Here, using micro-milling, we engineered a multichannel diffusion device that incorporates a chemoattractant gradient and a supported lipid bilayer (SLB) tethered with membrane-bound factors that mimics stromal cell membranes. The chemoattractant channels are separated by hydrogel barriers from SLB in the cell loading channel, which enable precise control of timing and profile of the chemokine gradients applied on cells interacting with SLB. The hydrogel barriers are formed in pillar-free channels through a liquid pinning process, which eliminates complex cleanroom-based fabrications and distortion of chemoattractant gradient by pillars in typical microfluidic hydrogel barrier designs. As a proof-of-concept, we formed an SLB tethered with ICAM-1, and demonstrated its lateral mobility and different migratory behavior of Jurkat T cells on it from those on immobilized ICAM-1, under a gradient of chemokine CXCL12. Our platform can thus be widely used to investigate membrane-bound chemotaxis such as in cancer, immune, and stem cells. Full article
(This article belongs to the Special Issue Microfluidics Technologies for Cell-Based Assays, Volume II)
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