"Lab-on-a-Chip" Devices for Diagnostic Evaluation: A Change-Promoting Approach in the Analysis of Biomarkers in Biological Matrices

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology and Biomedicine".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 1807

Special Issue Editors


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Guest Editor
Department of Precision Medicine, University of Campania “L. Vanvitelli”, Viale Abramo Lincoln, 5, 81100 Caserta, CE, Italy
Interests: biomarkers; early-diagnosis; uremic toxins; advanced glycation ends products; lanthionine; point of care testing; biosensors; electrochemical biosensors to monitor CKD progression; cardiovascular diseases in CKD; inflammation

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Guest Editor
CNR - SuPerconducting and Other INnovative Materials and Devices Institute, Napoli, NA, Italy
Interests: organic electronics and bioelectronics; chemical and biological sensing; field-effect and electrochemical transistors; organic–inorganic hybrid compounds; charge transport phenomena; film deposition techniques
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Guest Editor
Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università di Napoli Federico II, 80125 Napoli, Italy
Interests: lab-on-a chip; biosensors; microfluidics; viscoelasticity; blood; fluid dynamics and microstructure of complex fluids

Special Issue Information

Dear Colleagues,

We are pleased to announce a Special Issue of Micromachines focusing on “Lab-on-a-chip" Devices for Diagnostic Evaluation: A Change-Promoting Approach in The Analysis of Biomarkers in Biological Matrices.

In recent years, a growing scientific interest has emerged, in various fields of biomedicine, in the applications of portable biomarker-detection systems. These technologies refer to Point of Care Testing (POCT) concept, i.e., compact instrumentation, suitable for performing rapid tests. POCTs enable the quick collection of patients’ health information, favoring a more rapid medical decision making, thus improving care of patients. Moreover, POCTs allow a better management of the Health System economic burden. Various operational principles and upmost promising biosensing applications exist: electrochemicals, nanoelectronics, microfluidics and enzyme-based biosensors, as well as photonic sensor technologies. Besides of the operational principles, biosensors harness the recognition capabilities between biomolecules (ex. antigene-antibody, receptor-ligand, etc…) and combine them with transducers to deliver simple, easy-to-use diagnostic systems for an ever-expanding range of applications to improve health, well-being and the environment.

This Special Issue is aimed at providing selected contributions on advances in the biosensors field, including their production, characterization, as well as protocols standardization in the presence of biological samples.

Dr. Margherita Borriello
Dr. Mario Barra
Dr. Valentina Preziosi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Point of Care Testing (POCT)
  • lab-on-a-chip and multiplexed sensors
  • better management of difficult patients
  • early diagnosis
  • biosensors for novel biomarker
  • better management of the health system economic burden
  • rapid turn-around-time
  • POCT for emergency unit
  • nanoebiosensors
  • microfluidics and immobilisation technology
  • enzyme-based biosensors
  • bioelectronic interfaces
  • organic electrolyte-gated field-effect transistors
  • organic electrochemical transistors
  • screen printed electrodes

Published Papers (1 paper)

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Research

17 pages, 4402 KiB  
Article
Laser Bioprinting with Cell Spheroids: Accurate and Gentle
by Ekaterina D. Minaeva, Artem A. Antoshin, Nastasia V. Kosheleva, Polina I. Koteneva, Sergey A. Gonchukov, Svetlana I. Tsypina, Vladimir I. Yusupov, Peter S. Timashev and Nikita V. Minaev
Micromachines 2023, 14(6), 1152; https://doi.org/10.3390/mi14061152 - 30 May 2023
Cited by 3 | Viewed by 1509
Abstract
Laser printing with cell spheroids can become a promising approach in tissue engineering and regenerative medicine. However, the use of standard laser bioprinters for this purpose is not optimal as they are optimized for transferring smaller objects, such as cells and microorganisms. The [...] Read more.
Laser printing with cell spheroids can become a promising approach in tissue engineering and regenerative medicine. However, the use of standard laser bioprinters for this purpose is not optimal as they are optimized for transferring smaller objects, such as cells and microorganisms. The use of standard laser systems and protocols for the transfer of cell spheroids leads either to their destruction or to a significant deterioration in the quality of bioprinting. The possibilities of cell spheroids printing by laser-induced forward transfer in a gentle mode, which ensures good cell survival ~80% without damage and burns, were demonstrated. The proposed method showed a high spatial resolution of laser printing of cell spheroid geometric structures at the level of 62 ± 33 µm, which is significantly less than the size of the cell spheroid itself. The experiments were performed on a laboratory laser bioprinter with a sterile zone, which was supplemented with a new optical part based on the Pi-Shaper element, which allows for forming laser spots with different non-Gaussian intensity distributions. It is shown that laser spots with an intensity distribution profile of the “Two rings” type (close to Π-shaped) and a size comparable to a spheroid are optimal. To select the operating parameters of laser exposure, spheroid phantoms made of a photocurable resin and spheroids made from human umbilical cord mesenchymal stromal cells were used. Full article
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