Droplet Microfluidics, Volume II

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "A:Physics".

Deadline for manuscript submissions: closed (15 September 2021) | Viewed by 3474

Special Issue Editors

Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794, USA
Interests: single-cell analysis; single-cell genomics; droplet microfluidics; array of microwells
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Assistant Guest Editor
Cold Spring Harbor Laboratories, Cold Spring Harbor, NY 11724, USA
Interests: single-cell DNA sequencing; single-cell RNA sequencing; cancer genomics; bioinformatics

Special Issue Information

Dear Colleagues,

Droplet microfluidics has dramatically developed in the past decade, and has been established as one of the microfluidic technologies that can translate into commercial products. Its rapid development and adoption have relied not only on an efficient stabilizing system (oil and surfactant), but also on a library of modules that are able to manipulate droplets at a high-throughput. Droplet microfluidics is a vibrant field that keeps evolving, with advances that span technology development and applications. Recent examples include innovative methods to generate droplets, to perform single-cell encapsulation, magnetic extraction, or sorting at an even higher throughput. The trend consists of improving parameters such as robustness, throughput, or ease of use. Remarkably, these developments rely on a firm understanding of the physics and chemistry involved in capillary systems at a small scale. Finally, droplet microfluidics has played a pivotal role in biological applications, such as single-cell genomics or high-throughput microbial screening, and chemical applications.

This Special Issue seeks to showcase all of the aspects of the exciting field of droplet microfluidics, including, but not limited to, technology development, applications, and open-source systems.

Dr. Eric Brouzes
Dr. Siran Li
Guest Editors

Manuscript Submission Information

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Keywords

  • droplet microfluidics
  • droplet manipulation
  • droplet-based applications
  • chemistry of microfluidic droplets
  • physics of microfluidic droplets
  • open-source equipment for droplet microfluidics
  • droplet-based high-throughput screening
  • single-cell manipulation or analysis with droplet microfluidics

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Published Papers (1 paper)

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Research

11 pages, 1528 KiB  
Article
Simultaneous Droplet Generation with In-Series Droplet T-Junctions Induced by Gravity-Induced Flow
by Khashayar R. Bajgiran, Alejandro S. Cordova, Riad Elkhanoufi, James A. Dorman and Adam T. Melvin
Micromachines 2021, 12(10), 1211; https://doi.org/10.3390/mi12101211 - 4 Oct 2021
Cited by 4 | Viewed by 3044
Abstract
Droplet microfluidics offers a wide range of applications, including high-throughput drug screening and single-cell DNA amplification. However, these platforms are often limited to single-input conditions that prevent them from analyzing multiple input parameters (e.g., combined cellular treatments) in a single experiment. Droplet multiplexing [...] Read more.
Droplet microfluidics offers a wide range of applications, including high-throughput drug screening and single-cell DNA amplification. However, these platforms are often limited to single-input conditions that prevent them from analyzing multiple input parameters (e.g., combined cellular treatments) in a single experiment. Droplet multiplexing will result in higher overall throughput, lowering cost of fabrication, and cutting down the hands-on time in number of applications such as single-cell analysis. Additionally, while lab-on-a-chip fabrication costs have decreased in recent years, the syringe pumps required for generating droplets of uniform shape and size remain cost-prohibitive for researchers interested in utilizing droplet microfluidics. This work investigates the potential of simultaneously generating droplets from a series of three in-line T-junctions utilizing gravity-driven flow to produce consistent, well-defined droplets. Implementing reservoirs with equal heights produced inconsistent flow rates that increased as a function of the distance between the aqueous inlets and the oil inlet. Optimizing the three reservoir heights identified that taller reservoirs were needed for aqueous inlets closer to the oil inlet. Studying the relationship between the ratio of oil-to-water flow rates (Φ) found that increasing Φ resulted in smaller droplets and an enhanced droplet generation rate. An ANOVA was performed on droplet diameter to confirm no significant difference in droplet size from the three different aqueous inlets. The work described here offers an alternative approach to multiplexed droplet microfluidic devices allowing for the high-throughput interrogation of three sample conditions in a single device. It also has provided an alternative method to induce droplet formation that does not require multiple syringe pumps. Full article
(This article belongs to the Special Issue Droplet Microfluidics, Volume II)
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