eGenetics

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: closed (1 September 2019) | Viewed by 9759

Special Issue Editors


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Guest Editor
Catalog, Boston, MA, USA
Interests: data storage; DNA; blockchain; biosecurity;

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Co-Guest Editor
Nuclera Nucleics Ltd, Cambridge CB4 0GD, UK
Interests: enzymatic DNA synthesis; engineering biology; biosecurity

Special Issue Information

Dear Colleagues,

We are at the beginning of a revolution that will fundamentally alter the way we live, work, and interact with one another. Historically, revolutions are defined by significant changes in society, culture, politics, and technology. At the heart of this revolution is a molecule, DNA, of which the structure was first described 65 years ago and is currently transforming our world at an exponential rate. Lately, nucleic acid-based innovations have expanded beyond traditional biological fields like tool stacks for biological manufacturing or genome editing strategies for therapeutics and diagnostics. We are now seeing innovations in the computational environment to develop new approaches for data storage, computing, and recently blockchain. A whole new field is growing from the interaction between genetics and computer science with exciting possibilities, which can change the shape of our society in the forthcoming decades. In this issue, we invite pioneers in these fields to discuss their research and the opportunities that DNA and computation can provide for our future. Additionally, we also encourage participation from leaders in these fields who recognize the transformative nature of this technology but are concerned about the safety, security, and impact to our global community.

This Special Issue of Genes is open to researchers willing to contribute reviews, primary research, or commentary that highlight technological advantages as well as safety issues created from the interaction between DNA and computation.

Dr. Devin Leake
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosecurity
  • gene editing
  • CRISPR
  • data storage
  • DNA
  • computing, personalized medicine
  • blockchain
  • cryptocurrency
  • nanomaterial
  • synthetic biology
  • genome engineering

Published Papers (1 paper)

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Research

9 pages, 514 KiB  
Communication
Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3′ Terminal Structures for Enzymatic De Novo DNA Synthesis
by Sebastian Barthel, Sebastian Palluk, Nathan J. Hillson, Jay D. Keasling and Daniel H. Arlow
Genes 2020, 11(1), 102; https://doi.org/10.3390/genes11010102 - 16 Jan 2020
Cited by 25 | Viewed by 9293
Abstract
Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable the de novo synthesis of long oligonucleotides under mild, aqueous conditions. Intermediates with a 3′ terminal structure (hairpins) will inevitably arise during synthesis, but TdT has poor [...] Read more.
Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable the de novo synthesis of long oligonucleotides under mild, aqueous conditions. Intermediates with a 3′ terminal structure (hairpins) will inevitably arise during synthesis, but TdT has poor activity on these structured substrates, limiting its usefulness for oligonucleotide synthesis. Here, we described two parallel efforts to improve the activity of TdT on hairpins: (1) optimization of the concentrations of the divalent cation cofactors and (2) engineering TdT for enhanced thermostability, enabling reactions at elevated temperatures. By combining both of these improvements, we obtained a ~10-fold increase in the elongation rate of a guanine-cytosine hairpin. Full article
(This article belongs to the Special Issue eGenetics)
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