Genome Sequence of Novel Bacteria Showing Potential Biotechnological Applications

A special issue of Data (ISSN 2306-5729). This special issue belongs to the section "Computational Biology, Bioinformatics, and Biomedical Data Science".

Deadline for manuscript submissions: 5 July 2024 | Viewed by 3044

Special Issue Editors


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Instituto de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain
Interests: biological control of insect pests; crop protection; entomopathohenic bacteria; Bacillus thuriniensis; Xenorhabdus spp.; insecticidal proteins; pore forming toxins; insect resistance
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Guest Editor
Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Microbiología y Zoología Agrícola (IMYZA), Hurlingham, Buenos Aires B1686, Argentina
Interests: Bacillus thuringiensis; microbial entomopathogens; insecticidal proteins; insect pest control; bioinsecticides
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Genetics, University of Valencia, 46100 Burjassot, Spain
Interests: Bacillus thuringiensis; insecticidal proteins; insect pest control; bioinsecticides
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Currently, next-generation sequencing (NGS) technologies constitute a cost-effective and time-saving tool for sequencing bacteria that harbor genes with potential biotechnological applications. These applications can be easily detected and predicted using a few bioinformatic tools on a regular desktop computer. From novel enzymes to exceptionally biologically active compounds and more, the bacterial genome serves as the starting point for discovering new species and valuable biotechnological tools. These tools not only simplify everyday tasks but also contribute to improvements in food, agriculture, and the environment, benefiting various end-users, the economy, and society as a whole. Over the past few years, the number of bacterial genomes, from draft genomes to complete chromosomes, has grown exponentially. This growth has enriched public databases with several thousands of annotated genomes that hold significant biological value. Additionally, publication of full-length articles addressing genomes and concise genome announcements have introduced novel species that were previously unreported, thereby enriching scientific literature.

In this Special Issue, we aim to present research on genome sequences of novel bacterial strains with promising and potential biotechnological applications. Our goal is to highlight registrable, and entirely new sequencing data to the field of biotechnology, thereby advancing related research areas.

Dr. Leopoldo Palma
Dr. Diego Herman Sauka
Prof. Dr. Baltasar Escriche
Guest Editor

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Keywords

  • next-generation sequencing
  • complete genome sequence
  • draft genome sequence
  • genome annotation
  • gene prediction
  • species identification
  • species classification
  • plasmids
  • gene clusters

Published Papers (2 papers)

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8 pages, 854 KiB  
Data Descriptor
Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
by Leopoldo Palma, Leila Ortiz, José Niz, Marcelo Berretta and Diego Sauka
Data 2024, 9(3), 40; https://doi.org/10.3390/data9030040 - 28 Feb 2024
Viewed by 1115
Abstract
The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome [...] Read more.
The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests. Full article
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6 pages, 197 KiB  
Data Descriptor
Draft Genome Sequence of the Commercial Strain Rhizobium ruizarguesonis bv. viciae RCAM1022
by Olga A. Kulaeva, Evgeny A. Zorin, Anton S. Sulima, Gulnar A. Akhtemova and Vladimir A. Zhukov
Data 2024, 9(2), 19; https://doi.org/10.3390/data9020019 - 23 Jan 2024
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Abstract
Legume plants enter a symbiosis with soil nitrogen-fixing bacteria (rhizobia), thereby gaining access to assimilable atmospheric nitrogen. Since this symbiosis is important for agriculture, biofertilizers with effective strains of rhizobia are created for crop legumes to increase their yield and minimize the amounts [...] Read more.
Legume plants enter a symbiosis with soil nitrogen-fixing bacteria (rhizobia), thereby gaining access to assimilable atmospheric nitrogen. Since this symbiosis is important for agriculture, biofertilizers with effective strains of rhizobia are created for crop legumes to increase their yield and minimize the amounts of mineral fertilizers required. In this work, we sequenced and characterized the genome of Rhizobium ruizarguesonis bv. viciae strain RCAM1022, a component of the ‘Rhizotorfin’ biofertilizer produced in Russia and used for pea (Pisum sativum L.). Full article
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