3D Cell Culture in Disease Modeling and Tissue Regeneration - 2nd Edition

Dear Colleagues,

The introduction of the third dimension in cell culture has completely changed the way in which cells and stem cells simulate diseases and facilitate in vitro fabrication to replace tissue blocks. The preservation of histological features, such as the presence of the surrounding stromal niche, intercellular heterotypic connections, and mechanical stimulation, is essential for mimicking organ functionality. New techniques, biomaterials, and emerging platforms that improve 3D reconstruction of the in vivo environment represent a tremendous advantage for achieving further progress in biomedicine and the bioengineering future. The application of physiologically relevant culture models that have been invented and tested by researchers has potential in the development of more effective therapeutic treatments. These cell–cell or cell–biomaterial systems offer a cost-effective option for testing pharmaceutical and cosmetic products.
This Special Issue aims to collect articles detailing the contribution of 3D cell culture to uncovering novel insights into the molecular mechanisms regulating pathogenesis in the advancement of cell-based biofabrication and in enabling regenerative medicine strategies. Original research articles, review articles, and short communications are welcome(Volume 1, 13 papers: https://www.mdpi.com/journal/bioengineering/special_issues/cell_regeneration)

Potential topics include but are not limited to the following:

• Development or application of in vitro 3D disease models;
• Novel co-culture protocols and multicellular systems;
• Evaluation of tumor/cancer spheroids (tumoroids);
• Three-dimensional culture of stem cells;
• Organoids and organotypic cultures;
• New tissue engineering strategies;
• Bioprinted cell-based models;
• Biomimetic scaffold development and biocompatibility;
• Novel biomaterials used in preclinical or clinical studies;
• Validation of drugs and compounds in 3D in vitro systems;
• Testing of natural compounds in 3D models;
• Organ-on-a-chip and cell-based sensors;
• Fluidic and label-free approaches for 3D modeling;
• Standardization of 3D models

Dr. Pasquale Marrazzo
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• 3D cell culture 3D cell model tissue engineering stem cells biomaterials disease model spheroids organoids scaffold