Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.2
4.6
Journals
Bioengineering
Special Issues
Yeast Biotechnology: Current Challenges and Future Directions
share announcement

Yeast Biotechnology: Current Challenges and Future Directions

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Biochemical Engineering".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 4305

Special Issue Editors

Dr. Dongming Xie

E-Mail Website
Guest Editor
Chemical Engineering, University of Massachusetts Lowell, Lowell, MA 01854, USA
Interests: metabolic engineering; fermentation engineering; biomanufacturing; Yarrowia lipolytica; biorecycling of plastic wastes; process scale-up; bioprocess modeling
Dr. Quinn Zhu

E-Mail Website
Guest Editor
International Flavors and Fragrances, Wilmington, DE 19805, USA
Interests: yeast biotechnology; molecular biology; synthetic biology; metabolic engineering
Dr. Edward S. Miller, Jr.

E-Mail Website
Guest Editor
International Flavors and Fragrances, Wilmington, DE 19805, USA
Interests: bioprocess engineering; yeast biotechnology; biological systems analysis and modeling; technoeconomic analyses

Special Issue Information

Dear Colleagues,

The use of yeast in biotechnology has a long and fascinating history, dating back thousands of years. It is one of the major microorganisms that has been discovered by humans and utilized for benefiting our daily lives. With advances in modern biotechnology, microbiology, synthetic biology and metabolic engineering, yeast has been used to manufacture biofuels, commodity and specialty chemicals, industrial enzymes, food ingredients and pharmaceuticals. Compared with other major microorganisms, yeast is a type of single-celled microorganism that has several advantages as a host for biomanufacturing, which include (1) relatively easy to genetically manipulate, (2) available knowledge and biotechnology tools in both strain engineering and fermentation process development, (3) fast growth rate and capability for high-density fermentation, (4) ease of cultivation with simple media and process conditions, (5) high secretion capacity for product accumulation and downstream recovery, and (6) high biological safety.

This Special Issue of Bioengineering will focus on current challenges and future directions of yeast biotechnology, which includes: fundamental and applied research on yeast biology; metabolic engineering; molecular biology and genetic engineering; new products from yeast; new cell cultivation and fermentation methods; downstream process development and product recovery; bioprocess scale-up; techno-economic analysis of yeast biotechnology and products.      

Dr. Dongming Xie
Dr. Quinn Zhu
Dr. Edward S. Miller, Jr.
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • yeast biotechnology
  • yeast biology
  • synthetic biology
  • metabolic engineering

Published Papers (2 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

21 pages, 5882 KiB  
Article
A Cost-Effective Pichia pastoris Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins
by Jeffrey L. Schloßhauer, Srujan Kumar Dondapati, Stefan Kubick and Anne Zemella
Bioengineering 2024, 11(1), 92; https://doi.org/10.3390/bioengineering11010092 - 18 Jan 2024
Viewed by 1371
Abstract
Cell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation costs, and the supplementation of cell-free reactions with energy [...] Read more.
Cell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation costs, and the supplementation of cell-free reactions with energy regeneration systems. Consequently, the methylotrophic yeast Pichia pastoris, which is widely used in recombinant protein production, was utilized in the present study to realize cell-free synthesis in a cost-effective manner. Sensitive disruption conditions were evaluated, and appropriate signal sequences for translocation into ER vesicles were identified. An alternative energy regeneration system based on fructose-1,6-bisphosphate was developed and a ~2-fold increase in protein production was observed. Using a statistical experiment design, the optimal composition of the cell-free reaction milieu was determined. Moreover, functional ion channels could be produced, and a G-protein-coupled receptor was site-specifically modified using the novel cell-free system. Finally, the established P. pastoris cell-free protein production system can economically produce complex proteins for biotechnological applications in a short time. Full article
(This article belongs to the Special Issue Yeast Biotechnology: Current Challenges and Future Directions)
►▼ Show Figures

Figure 1

Review

Jump to: Research

41 pages, 3775 KiB  
Review
Perspectives for Using CO2 as a Feedstock for Biomanufacturing of Fuels and Chemicals
by Elif Kurt, Jiansong Qin, Alexandria Williams, Youbo Zhao and Dongming Xie
Bioengineering 2023, 10(12), 1357; https://doi.org/10.3390/bioengineering10121357 - 26 Nov 2023
Cited by 1 | Viewed by 2439
Abstract
Microbial cell factories offer an eco-friendly alternative for transforming raw materials into commercially valuable products because of their reduced carbon impact compared to conventional industrial procedures. These systems often depend on lignocellulosic feedstocks, mainly pentose and hexose sugars. One major hurdle when utilizing [...] Read more.
Microbial cell factories offer an eco-friendly alternative for transforming raw materials into commercially valuable products because of their reduced carbon impact compared to conventional industrial procedures. These systems often depend on lignocellulosic feedstocks, mainly pentose and hexose sugars. One major hurdle when utilizing these sugars, especially glucose, is balancing carbon allocation to satisfy energy, cofactor, and other essential component needs for cellular proliferation while maintaining a robust yield. Nearly half or more of this carbon is inevitably lost as CO2 during the biosynthesis of regular metabolic necessities. This loss lowers the production yield and compromises the benefit of reducing greenhouse gas emissions—a fundamental advantage of biomanufacturing. This review paper posits the perspectives of using CO2 from the atmosphere, industrial wastes, or the exhausted gases generated in microbial fermentation as a feedstock for biomanufacturing. Achieving the carbon-neutral or -negative goals is addressed under two main strategies. The one-step strategy uses novel metabolic pathway design and engineering approaches to directly fix the CO2 toward the synthesis of the desired products. Due to the limitation of the yield and efficiency in one-step fixation, the two-step strategy aims to integrate firstly the electrochemical conversion of the exhausted CO2 into C1/C2 products such as formate, methanol, acetate, and ethanol, and a second fermentation process to utilize the CO2-derived C1/C2 chemicals or co-utilize C5/C6 sugars and C1/C2 chemicals for product formation. The potential and challenges of using CO2 as a feedstock for future biomanufacturing of fuels and chemicals are also discussed. Full article
(This article belongs to the Special Issue Yeast Biotechnology: Current Challenges and Future Directions)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-2
Back to TopTop