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Bioengineering
Special Issues
Advanced Dynamic Cell and Tissue Culture, Volume 2
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Advanced Dynamic Cell and Tissue Culture, Volume 2

A special issue of Bioengineering (ISSN 2306-5354).

Deadline for manuscript submissions: closed (15 March 2019) | Viewed by 23819

Special Issue Editors

Prof. Dr. Cornelia Kasper

E-Mail Website
Guest Editor
Department of Biotechnology, University of Natural Resources and Life Science, Muthgasse 18, A-1190 Vienna, Austria
Interests: 3D cell culture; bioreactors; tissue engineering; biomaterials; stem cells; cell expansion and differentiation; dynamic cultivation; hypoxia
Special Issues, Collections and Topics in MDPI journals
Dr.-Ing. Jan Hansmann

E-Mail Website
Guest Editor
Head of Junior Research Group “Electronic-Tissue Interfaces”, Chair of Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg, Roentgenring 11, 97070 Wuerzburg
Special Issues, Collections and Topics in MDPI journals
Dr. Dominik Egger

E-Mail Website
Co-Guest Editor
Department of Biotechnology, University of Natural Resources and Life Science, Muthgasse 18, A-1190 Vienna, Austria
Interests: biotechnology; bioengineering; bioprocess engineering; 3D cell culture; tissue engineering; bioreactors; stem cell manufacturing; stem cells; biomaterials; scaffolds
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

“Classical” standard cell cultivation is still performed under static conditions on 2 D plastic surfaces in ambient atmosphere. These conditions do not mimic physiological in vivo conditions for human primary cells including stem and progenitor cells. For improving cell culture conditions and thus functionality of cells and tissues the expansion and differentiation should be performed in scalable bioreactors in/or on 3 D scaffolds under dynamic conditions. These bioreactor based bioprocesses can be automated and controlled resulting in optimized transport of nutrients and metabolic waste as well as in monitoring and control of the tissue microenvironment.

The current Special Issue wants to highlight new strategies for the development of bioprocesses for organ specific cell cultivation as well as stem cell expansion and differentiation. New designs for 3 D cell culture approaches including co-cultures and scalable systems shall be presented and applications are welcome from the whole field of cell based therapies and in vitro tests. This issue is open for papers addressing:

• 3 D biomaterials for cell and tissue culture
• Bioreactor design and optimization including automation
• Microfluidics (e.g. organ on a chip)
• Stem cell expansion and differentiation including tissue engineering approaches
• Application of physiological conditions including mechanical stimulation
• Sensors and monitoring devices in 3 D cell cultures

We look forward to receiving your contributions for this Special Issue.

Prof. Dr. Cornelia Kasper
Dr. -Ing. Jan Hansmann
Dr. Dominik Egger
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Bioreactors
  • 3 D Cell Culture
  • Tissue Engineering
  • Microfluidics/Organ on a chip
  • Stem Cells
  • Biomaterials
  • Automation

Published Papers (3 papers)

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Research

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20 pages, 7549 KiB  
Article
A Microcavity Array-Based 4D Cell Culture Platform
by Cordula Nies, Tobias Rubner, Hanna Lorig, Vera Colditz, Helen Seelmann, Andreas Müller and Eric Gottwald
Bioengineering 2019, 6(2), 50; https://doi.org/10.3390/bioengineering6020050 - 31 May 2019
Cited by 5 | Viewed by 7140
Abstract
(1) Background: We describe a 4D cell culture platform with which we tried to detect and to characterize migration dynamics of single hematopoietic stem cells in polymer film microcavity arrays integrated into a microtiter plate. (2) Methods: The system was set up with [...] Read more.
(1) Background: We describe a 4D cell culture platform with which we tried to detect and to characterize migration dynamics of single hematopoietic stem cells in polymer film microcavity arrays integrated into a microtiter plate. (2) Methods: The system was set up with CD34-expressing KG-1a cells as a surrogate for hematopoietic stem cells. We then evaluated the system as an artificial hematopoietic stem cell niche model comprised of a co-culture of human hematopoietic stem cells from cord blood (cord blood CD34+ cells, hHSCs) and human mesenchymal stromal cells (hMSCs) from bone marrow over a period of 21 days. We used a software-based cell detection method to count single hematopoietic stem cells (HSCs) in microcavities. (3) Results: It was possible to detect single HSCs and their migration behavior within single microcavities. The HSCs displayed a pronounced migration behavior with one population of CD34-expressing cells located at the bottom of the microcavities and one population located in the middle of the microcavities at day 14. However, at day 21 the two populations seemed to unite again so that no clear distinction between the two was possible anymore. (4) Conclusions: Single cell migration detection was possible but microscopy and flow cytometry delivered non-uniform data sets. Further optimization is currently being developed. Full article
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture, Volume 2)
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30 pages, 16401 KiB  
Article
Growth Behavior of Human Adipose Tissue-Derived Stromal/Stem Cells at Small Scale: Numerical and Experimental Investigations
by Valentin Jossen, Regine Eibl, Matthias Kraume and Dieter Eibl
Bioengineering 2018, 5(4), 106; https://doi.org/10.3390/bioengineering5040106 - 04 Dec 2018
Cited by 12 | Viewed by 6588
Abstract
Human adipose tissue-derived stromal/stem cells (hASCs) are a valuable source of cells for clinical applications, especially in the field of regenerative medicine. Therefore, it comes as no surprise that the interest in hASCs has greatly increased over the last decade. However, in order [...] Read more.
Human adipose tissue-derived stromal/stem cells (hASCs) are a valuable source of cells for clinical applications, especially in the field of regenerative medicine. Therefore, it comes as no surprise that the interest in hASCs has greatly increased over the last decade. However, in order to use hASCs in clinically relevant numbers, in vitro expansion is required. Single-use stirred bioreactors in combination with microcarriers (MCs) have shown themselves to be suitable systems for this task. However, hASCs tend to be less robust, and thus, more shear sensitive than conventional production cell lines for therapeutic antibodies and vaccines (e.g., Chinese Hamster Ovary cells CHO, Baby Hamster Kidney cells BHK), for which these bioreactors were originally designed. Hence, the goal of this study was to investigate the influence of different shear stress levels on the growth of humane telomerase reversed transcriptase immortalized hASCs (hTERT-ASC) and aggregate formation in stirred single-use systems at the mL scale: the 125 mL (=SP100) and the 500 mL (=SP300) disposable Corning® spinner flask. Computational fluid dynamics (CFD) simulations based on an Euler–Euler and Euler–Lagrange approach were performed to predict the hydrodynamic stresses (0.06–0.87 Pa), the residence times (0.4–7.3 s), and the circulation times (1.6–16.6 s) of the MCs in different shear zones for different impeller speeds and the suspension criteria (Ns1u, Ns1). The numerical findings were linked to experimental data from cultivations studies to develop, for the first time, an unstructured, segregated mathematical growth model for hTERT-ASCs. While the 125 mL spinner flask with 100 mL working volume (SP100) provided up to 1.68 × 105 hTERT-ASC/cm2 (=0.63 × 106 living hTERT-ASCs/mL, EF 56) within eight days, the peak living cell density of the 500 mL spinner flask with 300 mL working volume (SP300) was 2.46 × 105 hTERT-ASC/cm2 (=0.88 × 106 hTERT-ASCs/mL, EF 81) and was achieved on day eight. Optimal cultivation conditions were found for Ns1u < N < Ns1, which corresponded to specific power inputs of 0.3–1.1 W/m3. The established growth model delivered reliable predictions for cell growth on the MCs with an accuracy of 76–96% for both investigated spinner flask types. Full article
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture, Volume 2)
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Review

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24 pages, 1820 KiB  
Review
Expansion Culture of Human Pluripotent Stem Cells and Production of Cardiomyocytes
by Minh Nguyen Tuyet Le and Kouichi Hasegawa
Bioengineering 2019, 6(2), 48; https://doi.org/10.3390/bioengineering6020048 - 24 May 2019
Cited by 18 | Viewed by 9619
Abstract
Transplantation of human pluripotent stem cell (hPSCs)-derived cardiomyocytes for the treatment of heart failure is a promising therapy. In order to implement this therapy requiring numerous cardiomyocytes, substantial production of hPSCs followed by cardiac differentiation seems practical. Conventional methods of culturing hPSCs involve [...] Read more.
Transplantation of human pluripotent stem cell (hPSCs)-derived cardiomyocytes for the treatment of heart failure is a promising therapy. In order to implement this therapy requiring numerous cardiomyocytes, substantial production of hPSCs followed by cardiac differentiation seems practical. Conventional methods of culturing hPSCs involve using a 2D culture monolayer that hinders the expansion of hPSCs, thereby limiting their productivity. Advanced culture of hPSCs in 3D aggregates in the suspension overcomes the limitations of 2D culture and attracts immense attention. Although the hPSC production needs to be suitable for subsequent cardiac differentiation, many studies have independently focused on either expansion of hPSCs or cardiac differentiation protocols. In this review, we summarize the recent approaches to expand hPSCs in combination with cardiomyocyte differentiation. A comparison of various suspension culture methods and future prospects for dynamic culture of hPSCs are discussed in this study. Understanding hPSC characteristics in different models of dynamic culture helps to produce numerous cells that are useful for further clinical applications. Full article
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture, Volume 2)
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