Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.2
4.6
Journals
Bioengineering
Special Issues
Recent Advances in Mesenchymal Stem/Stromal Cell Processes
share announcement

Recent Advances in Mesenchymal Stem/Stromal Cell Processes

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Biofabrication and Biomanufacturing".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 1272

Special Issue Editor

Prof. Dr. Denise Salzig

E-Mail Website
Guest Editor
Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstrasse 14, 35390 Giessen, Germany
Interests: stem cell bioprocessing; bioreactor concepts for stem cells; up- and downstream processing for oncolytic measles virus; online monitoring and control of stem cell and viral production processes
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Mesenchymal stem/stromal cells (MSCs) are currently one of the most commonly used cell types in bioengineering and biomedicine. The purpose of this Special Issue is to provide a collection of high-quality research articles and reviews covering the latest knowledge of the development of mesenchymal stem/stromal cell processes. The scope of this Special Issue includes but is not limited to the following topics:

  • Manufacturing of stem cells as cell therapy products for human and animal use;
  • Manufacturing of stem cells in the context of cultured meat;
  • Processes for extracellular vesicle production with stem cells;
  • Up- and downstream development for stem cells, including the adaption of existing processes and process intensification;
  • Quality-by-design approaches;
  • Solutions for manufacturing challenges involved in stem cell processes;
  • Theoretical process set-up (bioengineering calculations, CFD simulations);
  • Novel bioreactor systems for stem cells;
  • Novel or sophisticated analytical methods for stem cells (those regarding process development are preferred);
  • Online monitoring tools and process automation for stem cells.

Prof. Dr. Denise Salzig
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • mesenchymal stem/stromal cells (MSCs)
  • stem cell processes
  • bioreactor systems
  • extracellular vesicle
  • intercellular interactions
  • regenerative medicine

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

18 pages, 2938 KiB  
Article
Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures
by Marialaura Madrigal, Patricia L. Fernández, Ricardo Lleonart, Lizmar Carreño, Kaiser Alejandro Villalobos Gorday, Ellís Rodríguez, Kathya de Cupeiro, Carlos M. Restrepo, K. S. Jagannatha Rao and Neil H. Riordan
Bioengineering 2023, 10(8), 930; https://doi.org/10.3390/bioengineering10080930 - 04 Aug 2023
Cited by 1 | Viewed by 1119
Abstract
Mesenchymal stromal cell (MSC)-derived products, such as trophic factors (MTFs), have anti-inflammatory properties that make them attractive for cell-free treatment. Three-dimensional (3D) culture can enhance these properties, and large-scale expansion using a bioreactor can reduce manufacturing costs. Three lots of MTFs were obtained [...] Read more.
Mesenchymal stromal cell (MSC)-derived products, such as trophic factors (MTFs), have anti-inflammatory properties that make them attractive for cell-free treatment. Three-dimensional (3D) culture can enhance these properties, and large-scale expansion using a bioreactor can reduce manufacturing costs. Three lots of MTFs were obtained from umbilical cord MSCs produced by either monolayer culture (Monol MTF) or using a 3D microcarrier in a spinner flask dynamic system (Bioreactor MTF). The resulting MTFs were tested and compared using anti-inflammatory potency assays in two different systems: (1) a phytohemagglutinin-activated peripheral blood mononuclear cell (PBMNC) system and (2) a lipopolysaccharide (LPS)-activated macrophage system. Cytokine expression by macrophages was measured via RT-PCR. The production costs of hypothetical units of anti-inflammatory effects were calculated using the percentage of TNF-α inhibition by MTF exposure. Bioreactor MTFs had a higher inhibitory effect on TNF (p < 0.01) than monolayer MTFs (p < 0.05). The anti-inflammatory effect of Bioreactor MTFs on IL-1β, TNF-α, IL-8, IL-6, and MIP-1 was significantly higher than that of monolayer MTFs. The production cost of 1% inhibition of TNF-α was 11–40% higher using monolayer culture compared to bioreactor-derived MTFs. A 3D dynamic culture was, therefore, able to produce high-quality MTFs, with robust anti-inflammatory properties, more efficiently than monolayer static systems. Full article
(This article belongs to the Special Issue Recent Advances in Mesenchymal Stem/Stromal Cell Processes)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop