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Bioengineering
Special Issues
Microtissues in Cell Culture, 3D Printing and Tissue Engineering
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Microtissues in Cell Culture, 3D Printing and Tissue Engineering

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Biofabrication and Biomanufacturing".

Deadline for manuscript submissions: 15 July 2024 | Viewed by 1753

Special Issue Editors

Dr. Kurt Pfannkuche

E-Mail Website
Guest Editor
Marga-and-Walter-Boll Laboratory for Cardiac Tissue Engineering, Center for Physiology and Pathophysiology, Medical Faculty, University of Cologne, Cologne, Germany
Interests: induced pluripotent stem cells; cardiac tissue engineering; cardiac cell therapy; microtissues and organoids
Dr. Nadine Nottrodt

E-Mail Website
Guest Editor
Fraunhofer Institute for Lasertechnology ILT, Aachen, Germany
Interests: laser; laser assisted bioprinting; LIFT; bioprinting; spheroid

Special Issue Information

Dear Colleagues,

Microtissues (MTs) have gained importance in the field of bioengineering in recent years. MTs can be composed of a single cell type or different cell types and thus exhibit very different degrees of complexity. Their size ranges from aggregates of a few cells to complex 3D assemblies of several hundred micrometers in diameter. In vitro, MTs are generated with or without a synthetic matrix and serve many different tasks. MTs can be used in cell culture to expand sensitive cells in 3D culture. Furthermore, MTs and especially organoids as very complex forms of MTs are used for the construction of in vitro test systems and the development of culture models to avoid animal experiments. MTs are also of particular interest as building blocks for 3D printing of tissues in vitro and transplantation in vivo in regenerative medicine applications. The current Special Issue brings MT-based bioengineering technologies into focus. Topics for this issue include manuscripts dealing with i.) scaffold-based and scaffold-free technologies to generate MTs in vitro, ii.) MTs as a technological platform to culture sensitive cells, iii.) technologies to print MTs via 3D tissue printing approaches, iv.) basic research approaches to understand cell–cell and cell–matrix communication as well as intracellular signaling in MTs, and v.) novel tools to analyze MTs including non-invasive readout suitable for high-throughput and longitudinal measurements.

Dr. Kurt Pfannkuche
Dr. Nadine Nottrodt
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microtissues
  • tissue engineering
  • 3D printing

Published Papers (2 papers)

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Research

14 pages, 2313 KiB  
Article
Development of a Probability-Based In Vitro Eye Irritation Screening Platform
by Seep Arora, Anna Goralczyk, Sujana Andra, Soon Yew John Lim and Yi-Chin Toh
Bioengineering 2024, 11(4), 315; https://doi.org/10.3390/bioengineering11040315 - 26 Mar 2024
Viewed by 626
Abstract
Traditional eye irritation assessments, which rely on animal models or ex vivo tissues, face limitations due to ethical concerns, costs, and low throughput. Although numerous in vitro tests have been developed, none have successfully reconciled the need for high experimental throughput with the [...] Read more.
Traditional eye irritation assessments, which rely on animal models or ex vivo tissues, face limitations due to ethical concerns, costs, and low throughput. Although numerous in vitro tests have been developed, none have successfully reconciled the need for high experimental throughput with the accurate prediction of irritation potential, attributable to the complexity of irritation mechanisms. Simple cell models, while suitable for high-throughput screening, offer limited mechanistic insights, contrasting with more physiologically relevant but less scalable complex organotypic corneal tissue constructs. This study presents a novel strategy to enhance the predictive accuracy of screening-compatible simple cell models in eye irritation testing. Our method combines the results of two in vitro assays—cell apoptosis and nociceptor (TRPV1) activation—using micropatterned chips to partition human corneal epithelial cells into numerous discrete small populations. Following exposure to test compounds, we measure apoptosis and nociceptor activation responses. The large datasets collected from the cell micropatterns facilitate binarization and statistical fitting to calculate a mathematical probability, which assesses the compound’s potential to cause eye irritation. This method potentially enables the amalgamation of multiple mechanistic readouts into a singular index, providing a more accurate and reliable prediction of eye irritation potential in a format amenable to high-throughput screening. Full article
(This article belongs to the Special Issue Microtissues in Cell Culture, 3D Printing and Tissue Engineering)
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24 pages, 9212 KiB  
Article
Contractile and Genetic Characterization of Cardiac Constructs Engineered from Human Induced Pluripotent Stem Cells: Modeling of Tuberous Sclerosis Complex and the Effects of Rapamycin
by Veniamin Y. Sidorov, Tatiana N. Sidorova, Philip C. Samson, Ronald S. Reiserer, Clayton M. Britt, M. Diana Neely, Kevin C. Ess and John P. Wikswo
Bioengineering 2024, 11(3), 234; https://doi.org/10.3390/bioengineering11030234 - 28 Feb 2024
Viewed by 858
Abstract
The implementation of three-dimensional tissue engineering concurrently with stem cell technology holds great promise for in vitro research in pharmacology and toxicology and modeling cardiac diseases, particularly for rare genetic and pediatric diseases for which animal models, immortal cell lines, and biopsy samples [...] Read more.
The implementation of three-dimensional tissue engineering concurrently with stem cell technology holds great promise for in vitro research in pharmacology and toxicology and modeling cardiac diseases, particularly for rare genetic and pediatric diseases for which animal models, immortal cell lines, and biopsy samples are unavailable. It also allows for a rapid assessment of phenotype–genotype relationships and tissue response to pharmacological manipulation. Mutations in the TSC1 and TSC2 genes lead to dysfunctional mTOR signaling and cause tuberous sclerosis complex (TSC), a genetic disorder that affects multiple organ systems, principally the brain, heart, skin, and kidneys. Here we differentiated healthy (CC3) and tuberous sclerosis (TSP8-15) human induced pluripotent stem cells (hiPSCs) into cardiomyocytes to create engineered cardiac tissue constructs (ECTCs). We investigated and compared their mechano-elastic properties and gene expression and assessed the effects of rapamycin, a potent inhibitor of the mechanistic target of rapamycin (mTOR). The TSP8-15 ECTCs had increased chronotropy compared to healthy ECTCs. Rapamycin induced positive inotropic and chronotropic effects (i.e., increased contractility and beating frequency, respectively) in the CC3 ECTCs but did not cause significant changes in the TSP8-15 ECTCs. A differential gene expression analysis revealed 926 up- and 439 down-regulated genes in the TSP8-15 ECTCs compared to their healthy counterparts. The application of rapamycin initiated the differential expression of 101 and 31 genes in the CC3 and TSP8-15 ECTCs, respectively. A gene ontology analysis showed that in the CC3 ECTCs, the positive inotropic and chronotropic effects of rapamycin correlated with positively regulated biological processes, which were primarily related to the metabolism of lipids and fatty and amino acids, and with negatively regulated processes, which were predominantly associated with cell proliferation and muscle and tissue development. In conclusion, this study describes for the first time an in vitro TSC cardiac tissue model, illustrates the response of normal and TSC ECTCs to rapamycin, and provides new insights into the mechanisms of TSC. Full article
(This article belongs to the Special Issue Microtissues in Cell Culture, 3D Printing and Tissue Engineering)
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