Dear Colleagues,

ES cells, iPS cells, and tissue stem cells are central cells in regenerative medicine. Recently, in the clinical application of regenerative medicine, attention has not only been focused on cells, but also on tissue regeneration after being secreted from cells. In the area of drug discovery, drug screening using iPS cells and immortalized cells has been widely reported. On the other hand, cancer stem cells also have a significant impact on cancer recurrence and metastasis. Cancer stem cells and mature cancer cells differ significantly in the metabolism of drugs (such as anticancer drugs). Furthermore, a variety of cells are associated with cancer stem cells and other cancer tissue surroundings. Alternative methods of animal experiments using cells for the activation of tissue stem cells, the evaluation of drugs (such as anti-cancer drugs), and the 3R principle are attracting attention within cell engineering.

This Special Issue invites submissions (reviews and originals) on a wide range of research studies, including on cell-based analysis, under the theme 'Regenerative Cell Engineering'.

This Special Issue includes, but is not limited to, the following:

• Regenerative medicine (ES cells, iPS cells, and tissue stem cells);
• Cancer therapy (cancer stem cells, cancer-associated fibroblasts, and cancer cell markers);
• Culture methods for tissue and cancer stem cells (2D culture, organoid culture, and xenografts);
• Cell-related drug screening;
• Alternative methods to animal experiments using cells.

Prof. Dr. Naoki Yamamoto
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• regenerative medicine
• cancer therapy
• culture method
• drug screening
• alternative methods of animal experiment