Animals

Research

Jump to: Review

23 pages, 1688 KiB

Open AccessArticle

Effect of Post-Ruminal Urea Supply on Growth Performance of Grazing Nellore Young Bulls at Dry Season

by Irene Alexandre Reis, Mailza Gonçalves de Souza, Yury Tatiana Granja-Salcedo, Isabela Pena Carvalho de Carvalho, Marco Aurélio De Felicio Porcionato, Laura Franco Prados, Gustavo Rezende Siqueira and Flávio Dutra De Resende

Animals 2023, 13(2), 207; https://doi.org/10.3390/ani13020207 - 06 Jan 2023

Cited by 3 | Viewed by 1629

Abstract

The objective of this study was to evaluate the effect of the use of post-ruminal urea on performance, nitrogen metabolism and the ruminal environment of Nellore cattle reared on pasture during the dry season. In experiment 1 (Exp. 1), nine ruminal-cannulated Nellore steers, [...] Read more.

The objective of this study was to evaluate the effect of the use of post-ruminal urea on performance, nitrogen metabolism and the ruminal environment of Nellore cattle reared on pasture during the dry season. In experiment 1 (Exp. 1), nine ruminal-cannulated Nellore steers, 30 ± 2 months old (651 ± 45 kg body weight (BW)), were allotted to a 3 × 3 Latin triple square. In experiment 2 (Exp. 2), 84 Nellore bulls, 18 ± 3 months old (315 ± 84 kg BW), were distributed in complete randomized blocks, by initial BW. Protein supplements were supplied daily, in the amount of 2 g/kg BW, and consisted of either CONT: protein + conventional urea (50% CP), PRU: protein + post ruminal urea (50% CP) and U + PRU: protein + urea conventional + post-ruminal urea (70% CP). The paddocks were composed of Urochloa brizantha cv. Marandu grass. In Exp. 1, there was no treatment effect for DM, OM, NDF, forage intake, and CP, but there was a higher intake for PRU (p < 0.005) and a higher digestibility for U+ PRU (p = 0.001). There was no effect on ruminal pH or NH₃-N concentration (p ≥ 0.232), but there was an interaction between treatment and time for them (p < 0.039). Furthermore, there was a treatment effect on the total SCFA concentration, with CONT being higher than the others. A difference in the acetate:propionate ratio was found (p < 0.027), with a greater relationship for PRU and U + PRU. A treatment effect (p = 0.049) was found for the propionate proportion, with a higher proportion in the CONT. Nitrogen intake was consequently lower for the CONT and higher urinary excretion for the U + PRU (p = 0.002). Animals supplemented with CONT showed a tendency to have more Bacteria and fewer Archaea (p = 0.086). In Exp. 2, there was a treatment effect for the disappearance rate of the supplement (p < 0.001). Intake was faster for PRU and CONT, but performance was not affected by PRU (p = 0.311). The use of post-ruminal urea alters the microbial population, but does not affect performance. Therefore, supplementation with post-ruminal urea presented similar results compared to conventional urea. Ruminal and blood parameters and animal performance were not influenced by treatments. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

16 pages, 4346 KiB

Open AccessArticle

Effect of 1,3-Beta Glucans Dietary Addition on the Growth, Intestinal Histology, Blood Biochemical Parameters, Immune Response, and Immune Expression of CD3 and CD20 in Broiler Chickens

by Shimaa A. Amer, Ghadeer A. Attia, Abed Alsalam Aljahmany, Aya K. Mohamed, Amer Al Ali, Ahmed Gouda, Gehan N. Alagmy, Hend M. Megahed, Taisir Saber and Mahmoud Farahat

Animals 2022, 12(22), 3197; https://doi.org/10.3390/ani12223197 - 18 Nov 2022

Cited by 7 | Viewed by 2016

Abstract

This experiment evaluated the impact of the dietary addition of 1,3-β-glucans (GLU) on broiler chickens’ growth, intestinal histology, blood biochemical parameters, and immunity. Two hundred three-day-old male broilers (Ross 308) (97.93 ± 0.19 g/chick) were randomly assigned into four treatments with five replicates, [...] Read more.

This experiment evaluated the impact of the dietary addition of 1,3-β-glucans (GLU) on broiler chickens’ growth, intestinal histology, blood biochemical parameters, and immunity. Two hundred three-day-old male broilers (Ross 308) (97.93 ± 0.19 g/chick) were randomly assigned into four treatments with five replicates, each containing ten birds, in a complete randomized design. The four treatments were formulated with 0, 50, 100, and 150 mg 1,3-β-glucans kg⁻¹ in broiler chicken diets. During the study, no significant impacts (p > 0.05) were observed in weight gain and feed conversion ratio (FCR) between treatment groups. Based on the results of total body weight gain and FCR, the optimal level of 1,3-β-glucan is 120 mg Kg⁻¹. The intestinal histomorphology was improved by GLU supplementation, as indicated by increased villi height and villi height to crypt depth ratio (p < 0.01). All levels of supplemental β-1,3 glucan decreased the serum total cholesterol (TC), triglyceride levels, and low-density lipoprotein cholesterol (LDL-C) (p < 0.05). The serum levels of growth hormones (GH), triiodothyronine (T3), and thyroxine (T4) were increased in GLU-supplemented groups (p < 0.05). The serum immune indices (lysozyme activity, interleukin 10 (IL10), complement 3 (C3), and total protein levels) were increased in the GLU-supplemented groups (p < 0.05). Dietary GLU up-regulated the immunoexpression of CD3 (T-cell marker) and CD20 (B-cell marker) in the spleen of birds (p < 0.01). It can be concluded that 1,3-β-glucan can be added to broiler chicken diets for improving the development and integrity of the intestine and enhancing the bird’s immune status. The optimal level for 1,3-β-glucan dietary supplementation was 120 mg Kg⁻¹. Dietary 1,3-β-glucan has a hypolipidemic effect and improves the hormonal profile of birds without affecting their growth rate. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

11 pages, 2800 KiB

Open AccessArticle

Cactus Cladodes Opuntia or Nopalea and By-Product of Low Nutritional Value as Solutions to Forage Shortages in Semiarid Areas

by Michelle C. B. Siqueira, Juana C. C. Chagas, João Paulo I. S. Monnerat, Carolina C. F. Monteiro, Robert E. Mora-Luna, Silas B. Felix, Milena N. Rabelo, Fernando L. T. Mesquita, Juliana C. S. Ferreira and Marcelo A. Ferreira

Animals 2022, 12(22), 3182; https://doi.org/10.3390/ani12223182 - 17 Nov 2022

Cited by 1 | Viewed by 1506

Abstract

We aimed to evaluate the effect of the cactus cladodes Nopalea cochenillifera (L). Salm-Dyck. (NUB) and cactus cladodes Opuntia stricta (Haw.) Haw. (OUB), both combined with sugarcane bagasse (SB) plus urea, Tifton hay (TH), corn silage (CS), and sorghum silage (SS) plus urea on [...] Read more.

We aimed to evaluate the effect of the cactus cladodes Nopalea cochenillifera (L). Salm-Dyck. (NUB) and cactus cladodes Opuntia stricta (Haw.) Haw. (OUB), both combined with sugarcane bagasse (SB) plus urea, Tifton hay (TH), corn silage (CS), and sorghum silage (SS) plus urea on nutrient intake and digestibility, ruminal dynamics, and parameters. Five male sheep, fistulated in the rumen, were assigned in a 5 × 5 Latin square design. The NUB provided a higher intake of dry matter (DM) and any nutrients than SS. TH provided larger pools of DM and iNDF. The OUB and CS provided a higher DM degradation. CS provided a higher NDF degradation rate. OUB provided a lower ruminal pH. Depending on the collection time, the lowest pH value was estimated at 3.79 h after the morning feeding. There was an interaction between treatments and collection time on VFA concentrations. Due to the high degradation rate, greater energy intake, less change in rumen pH, greater volatile fatty acid production, and feasibility, we recommend using cactus associated with sugarcane bagasse plus urea in sheep diets. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

9 pages, 542 KiB

Open AccessArticle

Effects of the Inclusion of Ground Pouteria sapota Kernel on Intake, Digestibility, and Growth Performance in Lambs

by Adriana Sánchez-Zárate, Alfonso J. Chay-Canul, Edgar Aguilar-Urquizo, J. Roberto Sanginés-García, Víctor Manuel Moo-Huchin, Einar Vargas-Bello-Pérez and Ángel T. Piñeiro-Vázquez

Animals 2022, 12(22), 3154; https://doi.org/10.3390/ani12223154 - 15 Nov 2022

Cited by 1 | Viewed by 1367

Abstract

This study determined the effect of replacing ground corn and soybean meal with ground Pouteria sapota kernel (PSSM) in lamb diets on nutrient intake and digestibility, performance, and carcass traits. Twenty-one male hair sheep lambs with an average body weight of 22 ± [...] Read more.

This study determined the effect of replacing ground corn and soybean meal with ground Pouteria sapota kernel (PSSM) in lamb diets on nutrient intake and digestibility, performance, and carcass traits. Twenty-one male hair sheep lambs with an average body weight of 22 ± 3.5 kg were randomly assigned to three treatment diets containing PSSM at 0, 10, and 20% of the total dry matter (DM) inclusion. The study lasted 60 days, which included 15 days for adaption and 45 days for sample collection. The PSSM inclusion did not affect intake or performance (p > 0.05). However, ether extract (EE) digestibility linearly increased (p < 0.0001), while crude protein (CP) and acid detergent fiber (ADF) linearly decreased. Final body weight, total weight gain, average daily weight gain, feeding efficiency, and carcass traits were not affected by PSSM inclusion. In conclusion, these results suggest that PSSM can replace up to 200 g/kg DM of ground corn and soybean meal without affecting intake or animal performance. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

14 pages, 1334 KiB

Open AccessArticle

Nutritional Evaluation of Black Soldier Fly Frass as an Ingredient in Florida Pompano (Trachinotus carolinus L.) Diets

by Amiti Banavar, Samad Keramat Amirkolaei, Lexi Duscher, Bela Haifa Khairunisa, Biswarup Mukhopadhyay, Michael Schwarz, Steve Urick and Reza Ovissipour

Animals 2022, 12(18), 2407; https://doi.org/10.3390/ani12182407 - 14 Sep 2022

Cited by 6 | Viewed by 2392

Abstract

The aquaculture industry is in need of sustainable fish feed to reduce the use of expensive and environmentally invasive wild-caught fish currently fed to many carnivorous species. The black soldier fly (BSF) has become a popular sustainable alternative protein source; however, the nutritional [...] Read more.

The aquaculture industry is in need of sustainable fish feed to reduce the use of expensive and environmentally invasive wild-caught fish currently fed to many carnivorous species. The black soldier fly (BSF) has become a popular sustainable alternative protein source; however, the nutritional waste byproduct of BSF, frass, has not been extensively studied as a feed replacement in carnivorous species. This study evaluates the potential of BSF frass on the growth, body composition, and intestinal microbiome of the Florida pompano, Trachinotus carolinus. Four experimental diets were formulated containing different levels of frass, replacing plant-based carbohydrate sources. As a result of this study, the frass did not improve the growth performance, resulting in a lower specific growth rate and higher feed conversion rate. While the frass diets did not alter the body composition, the visceral somatic index (VSI) significantly increased compared to the control diet and the hepatosomatic index (HIS) was lowered. The microbiome analysis showed high variation among the diets, with the control diet having the most distinct consortia, which may have been driven by the increased levels of starch compared to frass diets. This study indicates that BSF frass may not be a suitable feed replacement for carnivorous pompano; however, frass could still potentially be a replacement feed for herbivore or detritivore fish and should be further studied. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

10 pages, 313 KiB

Open AccessArticle

Giant Squid (Dosidicus gigas) Meal in Chicken Diets to Enrich Meat with n-3 Fatty Acids

by Jesús Morales-Barrera, María Carranco-Jáuregui, Guillermo Téllez-Isaías, Ana Sandoval-Mejía, Mariano González-Alcorta and Silvia Carrillo-Domínguez

Animals 2022, 12(17), 2210; https://doi.org/10.3390/ani12172210 - 27 Aug 2022

Viewed by 1338

Abstract

The main marketed parts of squid are the mantle, the head with tentacles, and fins. However, when the whole squid does not meet quality standards for human consumption it can be used for broiler feed. The objective of the study was to include [...] Read more.

The main marketed parts of squid are the mantle, the head with tentacles, and fins. However, when the whole squid does not meet quality standards for human consumption it can be used for broiler feed. The objective of the study was to include giant squid (Dosidicus gigas) meal (GSM) in broiler rations to increase the content of the n-3 fatty acids eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) in chicken meat. Two hundred Ross 380 chickens, half male, half female, and one day old, were randomly distributed in a 4x2x2 factorial arrangement. The factors were the treatment (0%, 1.67%, 3.34%, and 5.01% of GSM in the diet), sex, and content of n-3 in the legs with thighs and the breasts. Each treatment had five repetitions with 10 birds each. There were no differences (p > 0.05) in the production parameters for both sexes. The contents of EPA, DPA, and DHA increased in the females and in the legs with thighs (p < 0.05) with GSM. Acceptance for the flavor and texture of meat was higher in the treatment with 1.67% GSM than in the other treatments. It is concluded that GSM is an alternative for increasing the amount of n-3 in chicken meat. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

19 pages, 6356 KiB

Open AccessArticle

Effect of Black Soldier Fly (Hermetia illucens L.) Fat on Health and Productivity Performance of Dairy Cows

by Roman V. Nekrasov, Gennady A. Ivanov, Magomed G. Chabaev, Aloyna A. Zelenchenkova, Nadezhda V. Bogolyubova, Daria A. Nikanova, Alexander A. Sermyagin, Semen O. Bibikov and Sergey O. Shapovalov

Animals 2022, 12(16), 2118; https://doi.org/10.3390/ani12162118 - 18 Aug 2022

Cited by 5 | Viewed by 2433

Abstract

Due to the intensive development of technologies for obtaining protein, energy feed and biologically active supplements from insects, the feasibility and effectiveness of introducing these products into the rations of farm animals require further study. This research aims to study the possibility and [...] Read more.

Due to the intensive development of technologies for obtaining protein, energy feed and biologically active supplements from insects, the feasibility and effectiveness of introducing these products into the rations of farm animals require further study. This research aims to study the possibility and effects of feeding dairy cows fat from the larvae of the black soldier fly (BSFLF). The composition and properties of the BSFLF have been studied. The research of the fatty acid composition of BSFLF showed a high content of saturated fatty acids, including 58.9% lauric acid. The experiment was performed on black-and-white cows at the beginning of lactation (control, D0 (n = 12) vs. experimental D10 (n = 12) and D100 (n = 12) groups, 10 and 100 g/head/day BSFLF, respectively. There was no negative effect of BSFLF feeding on the process of feed digestion. The pH of the rumen content decreased (6.80 ± 0.07 & 6.85 ± 0.09 vs. 7.16 ± 0.06, p < 0.05), with an increase in the number of infusoria (0.27 ± 0.03&0.37 ± 0.09 vs. 0.18 ± 0.03 g/100 mL, p = 0.16); there was an increase in the concentration of VFA in the rumen content of animals of the experimental groups by 2.1 (p < 0.05) and 3.81 (p < 0.01) (8.66 ± 0.46 & 10.37 ± 0.42 vs. 6.56 ± 0.29) mmol/100 mL. The average daily milk yield of Group D10 cows over the experimental period (d17–d177) was slightly higher than the control (by 4.9%, p = 0.24 vs. Group D0). At the same time, Group D100 cows showed a significant increase in natural-fat milk compared to controls (by 8.0%, p < 0.05 vs. Group D0) over the same experiment period. Analysis of the fatty acid composition of the milk of the experimental animals showed some changes in the fatty acid composition of milk under the influence of BSFLF feeding; these changes were especially noticeable in Group D10. Thus, it was found that feeding dairy cows BSFLF at different dosages leads to better indicators of pre-gastric digestion and productivity. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

17 pages, 2768 KiB

Open AccessArticle

Supplementing a Phytogenic Feed Additive Modulates the Risk of Subacute Rumen Acidosis, Rumen Fermentation and Systemic Inflammation in Cattle Fed Acidogenic Diets

by Raul Rivera-Chacon, Ezequias Castillo-Lopez, Sara Ricci, Renee M. Petri, Nicole Reisinger and Qendrim Zebeli

Animals 2022, 12(9), 1201; https://doi.org/10.3390/ani12091201 - 06 May 2022

Cited by 12 | Viewed by 2562

Abstract

Feeding with high-concentrate diets increases the risk of subacute ruminal acidosis (SARA). This experiment was conducted to evaluate whether supplementing a phytogenic feed additive based on L-menthol, thymol, eugenol, mint oil (Mentha arvensis) and cloves powder (Syzygium aromaticum) (PHY) [...] Read more.

Feeding with high-concentrate diets increases the risk of subacute ruminal acidosis (SARA). This experiment was conducted to evaluate whether supplementing a phytogenic feed additive based on L-menthol, thymol, eugenol, mint oil (Mentha arvensis) and cloves powder (Syzygium aromaticum) (PHY) can amend the ruminal fermentation profile, modulate the risk of SARA and reduce inflammation in cattle. The experiment was designed as a crossover design with nine non-lactating Holstein cows, and was conducted in two experimental runs. In each run, cows were fed a 100% forage diet one week (wk 0), and were then transitioned stepwise over one week (0 to 65% concentrate, wk adapt.) to a high concentrate diet that was fed for 4 weeks. Animals were fed diets either with PHY or without (CON). The PHY group had an increased ruminal pH compared to CON, reduced time to pH < 5.8 in wk 3, which tended to decrease further in wk 4, reduced the ruminal concentration of D-lactate, and tended to decrease total lactate (wk 3). In wk 2, PHY increased acetate, butyrate, isobutyrate, isovalerate, and the acetate to propionate ratio compared to CON. Phytogenic supplementation reduced inflammation compared to CON in wk 3. Overall, PHY had beneficial effects on ruminal fermentation, reduced inflammation, and modulated the risk of SARA starting from wk 3 of supplementation. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

17 pages, 332 KiB

Open AccessArticle

Olive Pomace and Soybean-Sunflower Acid Oils as Alternative Fat Sources in European Seabass (Dicentrarchus labrax) Diets: Effects on Performance, Digestibility and Flesh Fatty Acid Composition and Quality Parameters

by Gerard Verge-Mèrida, Ana Cristina Barroeta, Carlos Ferrer, Tània Serrano, Francesc Guardiola, María Dolores Soler and Roser Sala

Animals 2022, 12(9), 1198; https://doi.org/10.3390/ani12091198 - 06 May 2022

Cited by 5 | Viewed by 2072

Abstract

The effects of dietary inclusion of soybean-sunflower and olive pomace acid oils on growth, digestibility and flesh composition were studied in European seabass. Eight diets were fed for 100 days (101.37 ± 0.33 g initial weight, mean ± SD), differing in the added [...] Read more.

The effects of dietary inclusion of soybean-sunflower and olive pomace acid oils on growth, digestibility and flesh composition were studied in European seabass. Eight diets were fed for 100 days (101.37 ± 0.33 g initial weight, mean ± SD), differing in the added fat source (25% fish oil, 75% experimental oil): S (crude soybean oil), SA (soybean-sunflower acid oil), O (crude olive pomace oil) or OA (olive pomace acid oil); 3 blends: S-O, S-OA, SA-OA at a 1:1 ratio; and a diet containing only fish oil (F) as a control. Animals fed OA showed the worst performance among dietary treatments, with the lowest weight, specific growth ratio, average daily gain and the highest feed conversion ratio (p < 0.01). In contrast, other diets including acid oils did not impair performance. Acid oil diets did not affect the apparent digestibility of dry matter, crude protein or total fatty acids (p > 0.05), but a lower digestibility of lipids and saturated fatty acids was observed (p < 0.001). Flesh composition and fatty acid profile were not affected by the high dietary free FA content (p > 0.05). Hence the results suggest that the studied acid oils may potentially be used in fish diets although further studies are needed. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

14 pages, 600 KiB

Open AccessArticle

Feeding Calcium-Ammonium Nitrate to Lactating Dairy Goats: Milk Quality and Ruminal Fermentation Responses

by Kleves V. Almeida, Geraldo T. Santos, Jesus A. C. Osorio, Jean C. S. Lourenço, Monique Figueiredo, Thomer Durman, Francilaine E. Marchi, Claudete R. Alcalde, Ranulfo C. Silva-Junior, Camila C. B. F. Itavo, Rafael C. Araujo and Andre F. Brito

Animals 2022, 12(8), 983; https://doi.org/10.3390/ani12080983 - 11 Apr 2022

Cited by 1 | Viewed by 1838

Abstract

We aimed to investigate the effects of calcium-ammonium nitrate (CAN) fed to lactating dairy goats on dry matter (DM) intake, digestibility of nutrients, milk properties (composition, antioxidant capacity, fatty acid profile, and nitrate residues), and ruminal fermentation parameters. Twelve lactating Saanen goats averaging [...] Read more.

We aimed to investigate the effects of calcium-ammonium nitrate (CAN) fed to lactating dairy goats on dry matter (DM) intake, digestibility of nutrients, milk properties (composition, antioxidant capacity, fatty acid profile, and nitrate residues), and ruminal fermentation parameters. Twelve lactating Saanen goats averaging 98.5 ± 13.1 days in milk, 53.5 ± 3.3 kg of body weight, and 2.53 ± 0.34 kg of milk/day were randomly assigned in four 3 × 3 Latin squares to receive the following diets: a control group (without CAN) with 7.3 g/kg DM of urea (URE), 10 g/kg DM of CAN (CAN10), and 20 g/kg DM of CAN (CAN20). Each period lasted 21 days, with 14 days for diet adaptation and seven days for data and sample collection. The DM intake, digestibility of nutrients, yields of milk, 3.5% fat-corrected milk, and energy-corrected milk were not affected by treatments. Similarly, there were no treatment effects on the yields and concentrations of milk fat, true protein, and lactose, along with minor effects on milk fatty acid profile. Total antioxidant capacity in milk was unaffected by treatments; however, concentration of conjugated dienes increased, while thiobarbituric acid reactive substances in milk decreased linearly. Nitrate and nitrite residues in milk were elevated by treatments, while the total of volatile fatty acids and ammonia-N concentration in the rumen were unaffected. Collectively, feeding CAN (up to 20 g/kg of DM) to lactating dairy goats did not affect feed intake, nutrient digestibility, and milk composition; however, it may increase milk lipid oxidation, as evidenced by increased conjugated diene concentration. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

12 pages, 277 KiB

Open AccessArticle

The Effect of Lithium Salt with Ascorbic Acid on the Antioxidant Status and Productivity of Gestating Sows

by Konstantin Ostrenko, Roman Nekrasov, Anastasiya Ovcharova, Viktar Lemiasheuski and Ivan Kutin

Animals 2022, 12(7), 915; https://doi.org/10.3390/ani12070915 - 02 Apr 2022

Cited by 3 | Viewed by 1867

Abstract

This research is aimed at the influence of different doses of lithium ascorbate on pigs’ diet estimation, at farrowing sows’ antioxidant status increase, and at lipid peroxidation product level decrease. The research was conducted in farrowing sows of the Irish landrace breed during [...] Read more.

This research is aimed at the influence of different doses of lithium ascorbate on pigs’ diet estimation, at farrowing sows’ antioxidant status increase, and at lipid peroxidation product level decrease. The research was conducted in farrowing sows of the Irish landrace breed during the second farrow. Three groups of animals were formed, with ten livestock units in each. Thirty days after successful insemination, the sows of the E10, E5 and E2 experimental groups started receiving lithium ascorbate powder together with feed stuff in dosages of 10, 5 and 2 mg/kg of body weight, respectively. Their weighing and biochemical examinations were performed before the substance introduction as well as on the 60th and 110th days of pregnancy. The following were detected in sows’ blood plasma: malondialdehyde, reduced glutathione, oxidized glutathione, SH/SS ratio, superoxide dismutase and glutathione peroxidase activity. Lithium ascorbate usage during sows’ breeding cycle caused a significant increase in SH (reduced glutathione) level by 21% (p < 0.05), SS (oxidized glutathione) level decrease by 17% (p < 0.05), and malondialdehyde level decrease by 60% (p < 0.05). These data outline antioxidant defense system activization, reducing the risk of oxidative stress under the influence of feeding with lithium ascorbate. Lithium ascorbate in dosages of 10 mg/kg per body weight given together with feed stuff shows prominent adaptogene and stress protective features in the most effective way. The research conducted regarding lithium ascorbate usage for farrowing sows can reduce the negative consequences of oxidative stress, increase sows’ health preservation level, and contribute to fertility boost. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

14 pages, 321 KiB

Open AccessArticle

Impact of Feeding Fermented Palm Kernel Cake and High Dietary Fat on Nutrient Digestibility, Enzyme Activity, Intestinal Morphology and Intestinal Nutrient Transporters mRNA Expression in Broiler Chickens under Hot and Humid Conditions

by Ali Hanafiah Hakim, Idrus Zulkifli, Abdoreza Soleimani Farjam, Elmutaz Atta Awad and Suriyah Kumari Ramiah

Animals 2022, 12(7), 882; https://doi.org/10.3390/ani12070882 - 31 Mar 2022

Cited by 4 | Viewed by 2258

Abstract

The study aimed at determining the ileal nutrient digestibility, digestive enzyme activity, intestinal morphology, and nutrient transporters mRNA expressions in broiler chickens fed with fermented PKC (LPKC) based diets with different levels of fat supplementation under hot and humid conditions. From day 22 [...] Read more.

The study aimed at determining the ileal nutrient digestibility, digestive enzyme activity, intestinal morphology, and nutrient transporters mRNA expressions in broiler chickens fed with fermented PKC (LPKC) based diets with different levels of fat supplementation under hot and humid conditions. From day 22 to 35, broiler chickens were randomly fed with either (1) 20% LPKC-based diet with 5% palm oil, (2) 20% LPKC based diet with 9.5% palm oil, (3) 20% PKC-based diet with 5% palm oil or (4) 20% PKC-based diet with 9.5% palm oil. Feeding LPKC and PKC diets at the finisher phase have not affected the nutrient’s digestibility, but a higher level of oil supplementation does. This was seconded by changes in the digestive enzyme activity, villus height, and mRNA expression of nutrient transporters in the higher level of oil-supplemented diets fed chickens. In conclusion, the inclusion of oil at 9.5% in a 20% LPKC/PKC-based diet is necessary to ensure better nutrient digestibility in chickens via improved digestive function, especially in hot and humid tropical regions. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

16 pages, 688 KiB

Open AccessArticle

Spineless Cactus plus Urea and Tifton-85 Hay: Maximizing the Digestible Organic Matter Intake, Ruminal Fermentation and Nitrogen Utilization of Wethers in Semi-Arid Regions

by Robert E. Mora-Luna, Ana M. Herrera-Angulo, Michelle C. B. Siqueira, Maria Gabriela da Conceição, Juana C. C. Chagas, Carolina C. F. Monteiro, Antonia S. C. Véras, Francisco F. R. Carvalho and Marcelo A. Ferreira

Animals 2022, 12(3), 401; https://doi.org/10.3390/ani12030401 - 08 Feb 2022

Cited by 3 | Viewed by 1865

Abstract

The aim of this study was to evaluate the effect of replacing Tifton-85 hay (Cynodon spp. cv. Tifton 85) with 0, 150, 300, 450 and 600 g/kg dry matter (DM) of spineless cactus (SC, Nopalea cochenilifera Salm-Dyck) plus urea and ammonium sulfate [...] Read more.

The aim of this study was to evaluate the effect of replacing Tifton-85 hay (Cynodon spp. cv. Tifton 85) with 0, 150, 300, 450 and 600 g/kg dry matter (DM) of spineless cactus (SC, Nopalea cochenilifera Salm-Dyck) plus urea and ammonium sulfate (UAS; 9:1) on DM, digestible organic matter (DOM) and indigestible neutral detergent fiber (iNDF) intakes, as well as ruminal fermentation, N-balance, and microbial protein supply (MPS). Five rumen-fistulated and cannulated crossbred wethers, weighing 43.8 ± 5.80 kg, were randomized in a 5 × 5 Latin square design. Isonitrogenous diets (14% crude protein) were supplied with a roughage/concentrate ratio of 70:30. The DOM intake, N-retained, and MPS showed quadratic responses (p < 0.05), with maximum values estimated at the levels of SC+UAS of 414, 438 and 418 g/kg DM, respectively. Rumen pH and ammonia nitrogen, iNDF intake, N-urinary excretion, and serum urea and plasma ammonia reduced linearly (p < 0.05) with increasing SC+UAS inclusion. Ruminal acetate and propionate concentrations increased linearly with increasing SC+UAS inclusion. In wethers fed diets with a roughage/concentrate ratio of 70:30, roughage constituted of a SC+UAS/hay (Tifton-85) ratio of 41:29 is recommended in order to maximize the DOM intake, N-retention, and MPS. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

9 pages, 228 KiB

Open AccessCommunication

Nutritive Value Variation of Paunch Manure as an Alternative Feed Ingredient

by Taylor Jacob Garcia, Nichole Marie Cherry, Kimberly Ann Guay, Jeffrey Alan Brady, James Pierre Muir and William Brandon Smith

Animals 2021, 11(12), 3573; https://doi.org/10.3390/ani11123573 - 16 Dec 2021

Cited by 2 | Viewed by 2672

Abstract

Ruminants, which have multi-compartmented stomachs, are adapted to digest cellulosic materials, which constitute the primary expense on ranches and dairies. Industrial byproducts can be repurposed for livestock diets to decrease these costs. Therefore, finding alternative feedstuffs may benefit the economics of livestock production. [...] Read more.

Ruminants, which have multi-compartmented stomachs, are adapted to digest cellulosic materials, which constitute the primary expense on ranches and dairies. Industrial byproducts can be repurposed for livestock diets to decrease these costs. Therefore, finding alternative feedstuffs may benefit the economics of livestock production. The goal of this project was to evaluate the variation in nutritive value of ruminal waste as a potential feedstuff. Twelve paunch samples were collected from individual cattle across multiple harvest dates at the Tarleton State University Meat Laboratory, Stephenville, TX. Samples were dried and assayed for dry matter (DM), crude protein (CP), sequential neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL), and physically-effective fiber (peNDF). Samples were subjected to batch-culture in vitro digestibility assays for the determination of digestibility coefficients. Mean NDF, ADF, ADL, CP and peNDF concentrations were 681, 399, 109, 150, and 387 g kg⁻¹ DM, respectively. Contribution to variance from sample for NDF, ADF, ADL, CP, and peNDF were 75.3, 41.9, 33.0, 51.2, and 71.3%, respectively. In vitro true digestibility (IVTD) and in vitro NDF digestibility (IVNDFD) were recorded as 462 and 216 g kg⁻¹ DM, respectively. Contribution to variation of sample for IVTD and IVNDFD were 31.0 and 30.7%, respectively. Results indicate that rumen waste harvested from abattoirs may be useful for sustainable livestock production, while reducing environmental threats posed by disposal, but the viability of the product is highly dependent on the source animal. For full viability of application in a sustainable system, a centralized receiving and compositing system may be useful for developing a consistent product. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

8 pages, 512 KiB

Open AccessCommunication

Nutritive Value Variation and In Vitro Digestibility of Hempseed Meal

by Kristen June Jacobson, Lea Ann Kinman, Walter Franklin Owsley, James Pierre Muir and William Brandon Smith

Animals 2021, 11(12), 3481; https://doi.org/10.3390/ani11123481 - 07 Dec 2021

Cited by 3 | Viewed by 2813

Abstract

Hempseed meal (HSM) is left after oil extraction of hemp and may act as a protein source in livestock. The first phase of this research evaluated variation in nutritive value and in vitro dry matter digestibility (IVDMD) of HSM from various sources in [...] Read more.

Hempseed meal (HSM) is left after oil extraction of hemp and may act as a protein source in livestock. The first phase of this research evaluated variation in nutritive value and in vitro dry matter digestibility (IVDMD) of HSM from various sources in North America; the second phase utilized IVDMD to evaluate the efficacy of hempseed meal as an ingredient in ruminant feed. In phase one, the source had no contribution to variance for neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), or crude protein (CP) (p ≥ 0.20). However, batch within source contributed to variation for NDF (50%), ADF (37%), ADL (13%), and CP (31%; p ≤ 0.01). Irrespective of differences in nutritive value, there was no contribution to variation (p = 0.23) of any measured response on in vitro true digestibility (53.0%). In phase two, two experiments evaluated HSM IVDMD as (1) a concentrate replacement or (2) a protein replacement in rations at varying rates. In the first experiment, IVDMD decreased (p < 0.05) with increasing levels of HSM. In the second experiment, IVDMD decreased (p < 0.01) as HSM inclusion increased. Although IVDMD decreased as HSM inclusion increased, values still met the digestibility threshold for ruminant rations, indicating that HSM has potential as an alternative protein ingredient. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

14 pages, 748 KiB

Open AccessArticle

Effect of Varying Levels of Hempseed Meal Supplementation on Humoral and Cell-Mediated Immune Responses of Goats

by Frank Abrahamsen, Gopal Reddy, Woubit Abebe and Nar Gurung

Animals 2021, 11(10), 2764; https://doi.org/10.3390/ani11102764 - 22 Sep 2021

Cited by 2 | Viewed by 1699

Abstract

The objective of this study was to evaluate the effect of varying levels of hempseed meal supplementation on antibody and cell-mediated immune responses, as well as the expression of some of the important immunoregulatory cytokines. Treatments consisted of hempseed meal supplementation at 0 [...] Read more.

The objective of this study was to evaluate the effect of varying levels of hempseed meal supplementation on antibody and cell-mediated immune responses, as well as the expression of some of the important immunoregulatory cytokines. Treatments consisted of hempseed meal supplementation at 0 (control), 10, 20, and 30% of the total diet. Goats were randomly assigned to one of the four treatments n = 10. Cell-mediated immune response was evaluated on day 59 of the feeding period by measuring skinfold thickness at 24 h following intradermal injection of phytohemagglutinin. A significant increase in skinfold thickness was observed with increasing levels of supplementation as compared to that of the control group. Serum antibody titers to chicken ovalbumin were not significantly different between treatment groups. Cytokine concentrations of IL-6 increased linearly with increasing level of supplementation (p < 0.05), contrarily to the linear decrease that was observed for TNF-α (p < 0.05). Although IL-2 tended to increase with the 10 and 30% levels of supplementation (p < 0.07), the result was not significant, and no significant differences were obtained with respect to IL-4 concentrations. Cytokine gene expression values measured by RT-PCR, however, demonstrated some significant differences. HSM supplementation had no significant effect on the expression of IL-2 or IL-6. However, significant differences were observed with the 30% supplementation for IL-4 and TNF-α as compared to that of the control group (p < 0.05). IL-4 was down regulated for the 10 and 20% treatment groups but was upregulated for the 30% treatment group. TNF-α was downregulated in the 10% but upregulated for the 20 and 30% treatment groups. No significant differences were observed for the serum cortisol concentration or white blood cell counts. These results suggested that hempseed meal supplementation may improve cell-mediated immune response while having no effect on antibody-mediated immune response. However, more research needs to be conducted to determine the most efficacious inclusion rate. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

18 pages, 7561 KiB

Open AccessArticle

Dietary Lactobacillus casei K17 Improves Lipid Metabolism, Antioxidant Response, and Fillet Quality of Micropterus salmoides

by Jinsong Wang, Zhuoying Zhu, Shenghao Tian, Huiyu Fu, Xiangjun Leng and Lanming Chen

Animals 2021, 11(9), 2564; https://doi.org/10.3390/ani11092564 - 31 Aug 2021

Cited by 5 | Viewed by 2175

Abstract

We previously demonstrated that Lactobacillus casei K17, isolated from Korean kimchi, has high antioxidant levels in vitro and in vivo. However, its effect on Micropterus salmoides is unknown. In this study, we investigated the impact of L. casei K17 supplementation on the lipid [...] Read more.

We previously demonstrated that Lactobacillus casei K17, isolated from Korean kimchi, has high antioxidant levels in vitro and in vivo. However, its effect on Micropterus salmoides is unknown. In this study, we investigated the impact of L. casei K17 supplementation on the lipid metabolism, antioxidant response, liver histology, and fillet quality of M. salmoides. We randomly assigned 450 M. salmoides (33.0 ± 0.5 g) to six diet groups for 69 days. The diets were as follows: 0.85% normal saline; 10% skim milk powder; 1 × 10⁸ CFU/g live L. casei K17 (LB); 1 × 10⁸ live L. casei K17 protected by skim milk powder (MB); 1 × 10⁸ dead L. casei K17 (DB); and L. casei K17 fermentation supernatant. MB significantly improved the crude protein, total collagen, alkaline-insoluble collagen, fiber numbers, hardness, chewiness, and gumminess of M. salmoides fillets (p < 0.05). LB significantly improved crude protein and fiber numbers (p < 0.05). Furthermore, dietary supplementation with LB, MB, and DB maintained normal liver histology, preserved liver function, and increased hepatic and hemal antioxidant status by enhancing antioxidant enzyme activities. Meanwhile, the three diets also promoted lipid metabolism by increasing HDL-C effectiveness and reducing total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels in serum and liver tissues, indicating that dietary supplementation with DB, LB, and MB had hypolipidemic effects on M. salmoides. MB and LB significantly improved fillet quality and LB, MB, and DB improved hemal and hepatic lipid metabolism and antioxidant response and reduced reactive oxygen species production, protecting M. salmoides hepatic cells from injury. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

13 pages, 328 KiB

Open AccessArticle

Miúda (Nopalea cochenillifera (L.) Salm-Dyck)—The Best Forage Cactus Genotype for Feeding Lactating Dairy Cows in Semiarid Regions

by Rubem R. Rocha Filho, Djalma C. Santos, Antonia S. C. Véras, Michelle C. B. Siqueira, Carolina C. F. Monteiro, Robert E. Mora-Luna, Lucas R. Farias, Viviany L. F. Santos, Juana C. Chagas and Marcelo A. Ferreira

Animals 2021, 11(6), 1774; https://doi.org/10.3390/ani11061774 - 14 Jun 2021

Cited by 5 | Viewed by 3520

Abstract

This study aimed to investigate the effects on nutrient intake and digestibility, milk yield (MY) and composition, milk fatty acids profile, and microbial protein synthesis caused by feeding lactating dairy cows four different forage cactus genotypes. Eight Girolando cows (5/8 Holstein × 3/8 [...] Read more.

This study aimed to investigate the effects on nutrient intake and digestibility, milk yield (MY) and composition, milk fatty acids profile, and microbial protein synthesis caused by feeding lactating dairy cows four different forage cactus genotypes. Eight Girolando cows (5/8 Holstein × 3/8 Gyr), weighing 490 ± 69.0 kg (means ± standard deviation), and producing 15.5 ± 1.0 kg/d of milk during pretrial were distributed to two contemporaneous 4 × 4 Latin squares. The cows were fed a total mixed ration composed of sorghum silage (385 g/kg of dry matter (DM)), concentrated mix (175 g/kg DM), and forage cactus (440 g/kg DM). The experimental treatments consisted of different cactus genotypes, such as Gigante cactus (GC), Miúda cactus (MC), IPA Sertânia cactus (SC), and Orelha de Elefante Mexicana cactus (OEMC). The feeding of MC provided a higher intake of DM, organic matter (OM), and total digestible nutrients, as well as higher MY, energy-corrected milk, and microbial protein synthesis in comparison with those resulting from the other genotypes tested. The GC promoted lower DM and OM, and the apparent digestibility of neutral detergent fiber. The cows fed with OEMC showed lower MY and milk protein yield and content, and higher unsaturated over saturated fatty acid ratio in milk. Miúda forage cactus increased nutrient intake, digestibility of DM and OM, and microbial synthesis without impairing the milk fatty acid profile. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

11 pages, 244 KiB

Open AccessArticle

Effects of 25-Hydroxyvitamin D₃ and Oral Calcium Bolus on Lactation Performance, Ca Homeostasis, and Health of Multiparous Dairy Cows

by Hongjian Xu, Quanyu Zhang, Lihua Wang, Chengrui Zhang, Yang Li and Yonggen Zhang

Animals 2021, 11(6), 1576; https://doi.org/10.3390/ani11061576 - 28 May 2021

Cited by 4 | Viewed by 3482

Abstract

Little information is available regarding the effect of supplementing 25-hydroxyvitamin D₃ during the transition period combined with a postpartum oral calcium bolus on Ca homeostasis. The objectives of the current study were to evaluate the effects of 25-hydroxyvitamin D₃ combined with [...] Read more.

Little information is available regarding the effect of supplementing 25-hydroxyvitamin D₃ during the transition period combined with a postpartum oral calcium bolus on Ca homeostasis. The objectives of the current study were to evaluate the effects of 25-hydroxyvitamin D₃ combined with postpartum oral calcium bolus on lactation performance, serum minerals and vitamin D₃ metabolites, blood biochemistry, and antioxidant and immune function in multiparous dairy cows. To evaluate the effects of 25-hydroxyvitamin D₃ combined with oral calcium, 48 multiparous Holstein cows were randomly assigned to one of four treatments: (1) supplementing 240 mg/day vitamin D₃ without a postpartum oral Ca bolus (control), (2) supplementing 240 mg/day vitamin D₃ with an oral Ca bolus containing 90 g of Ca immediately post-calving (Ca + VitD), (3) supplementing 6 g/day 25-hydroxyvitamin D₃ without an oral Ca bolus (25D), and (4) supplementing 6 g/day 25-hydroxyvitamin D₃ with an oral Ca bolus containing 90 g of Ca immediately post-calving (Ca + 25D). Lactation performance during the first 21 days was measured. Blood was collected at the initiation of calving and then 1, 2, 7, 14, and 21 days relative to the calving date. The yield of milk (0.05 < p < 0.10), energy-corrected milk (p < 0.05), 3.5% fat-corrected milk (p < 0.05), and milk protein (p < 0.05) were significantly higher in 25-hydroxyvitamin D₃-treated groups within 3 weeks of lactation than in vitamin D₃-treated cows. The iCa (p < 0.05) and tCa (p < 0.05) were higher in both Ca and 25D + Ca cows than in the control and 25D groups within 48 h. The concentrations of serum tCa (p < 0.05), tP (p < 0.05), and 25-hydroxyvitamin D₃ (p < 0.05) in 25D and 25D + Ca cows were higher than those in control and Ca cows within 21 days postpartum. Feeding 25-hydroxyvitamin D₃ also showed a lower concentration of malondialdehyde (p < 0.05), interleukin 6 (p < 0.05), and tumor necrosis factor-alpha (TNF-α) (p < 0.05), as well as a higher concentration of alkaline phosphatase (p < 0.05), total antioxidant capacity (p < 0.05), and immunoglobulin G (p < 0.05) than vitamin D₃. Supplementing Ca bolus also showed lower concentrations of alanine transaminase (p < 0.05) and TNF-α (p < 0.05). In conclusion, supplementing 25-hydroxyvitamin D₃ during the transition period combined with a postpartum oral calcium bolus improved lactation performance, Ca homeostasis, and antioxidant and immune function of medium-production dairy cows within 21 days postpartum. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

Review

Jump to: Research

15 pages, 1052 KiB

Open AccessReview

Opuntia spp. as Alternative Fodder for Sustainable Livestock Production

by Grazia Pastorelli, Valentina Serra, Camilla Vannuccini and Everaldo Attard

Animals 2022, 12(13), 1597; https://doi.org/10.3390/ani12131597 - 21 Jun 2022

Cited by 10 | Viewed by 3095

Abstract

During the past decades, livestock production has increased significantly, which has led to the degradation of rangelands due to overgrazing. The lack of water in several arid areas has led to a decline in crop and animal husbandry. As a consequence, the demand [...] Read more.

During the past decades, livestock production has increased significantly, which has led to the degradation of rangelands due to overgrazing. The lack of water in several arid areas has led to a decline in crop and animal husbandry. As a consequence, the demand for drought-resistant crops has increased significantly so as to keep crop and animal husbandry systems viable and sustainable. Cactaceae have adaptive characteristics that ensure their development progress under drought conditions. The present review provides information on the nutritive value of Opuntia in animal fodder production, its effects on animal performance, and the quality of the animal-derived products. In conclusion, the use of Opuntia as innovative alternative feed would render animal production systems more sustainable. Full article

(This article belongs to the Special Issue Advances in Animal Novel Alternative Feed)

► Show Figures

Figure 1

Journal Menu

Journal Browser

Advances in Animal Novel Alternative Feed

Share This Special Issue

Special Issue Editor

Special Issue Information

Keywords

Published Papers (20 papers)

Research

Review

Further Information

Guidelines

MDPI Initiatives

Follow MDPI