The Mammary Gland Identifies Genes and Networks in Dairy Livestock

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Genetics and Genomics".

Deadline for manuscript submissions: 15 November 2024 | Viewed by 2319

Special Issue Editor

College of Animal Sciences, Zhejiang University, Hangzhou, China
Interests: mammary gland development; fatty acid metabolism; gene function verification; signal pathway

Special Issue Information

Dear Colleagues,

The mammary gland is the most important economic organ of dairy livestock. With the development of society, especially the continuous growth of population, the worldwide demand for milk production increases constantly. An important challenge in this field is the improvement of the production performance of dairy animals using the advantage of animal nutrition and genetic breeding for the current industry. It is this challenge and consumer demand that have facilitated gene function research on the mammary gland. Recent evidence has suggested that lipid metabolism, amino acid metabolism, and carbohydrate metabolism greatly modify the development and lactation process of the mammary gland. With the advantage of a variety of omics techniques, a few genes and metabolic networks that regulate the process of development and lactation of mammary gland have been investigated. Even so, the remaining genes and gene networks that play a role in the mammary gland are unclear, hindering the process of improving dairy livestock production using genetic breeding and precision nutritional manipulation.

The aim of this Special Issue is therefore to improve our knowledge about the genes and gene pathways in dairy livestock, with special emphasis on the metabolism, development, and function of the mammary gland. We would particularly welcome manuscripts that assess gene function, new gene identification, gene networks related to metabolism, and the development of the mammary gland in dairy livestock.

Dr. Hengbo Shi
Guest Editor

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Keywords

  • dairy livestock
  • mammary gland
  • fatty acid metabolism
  • amino acid metabolism
  • glucose metabolism
  • gene function verification
  • gene identification
  • gene network

Published Papers (1 paper)

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Research

17 pages, 5121 KiB  
Article
Proteomics Insights into the Gene Network of cis9, trans11-Conjugated Linoleic Acid Biosynthesis in Bovine Mammary Gland Epithelial Cells
by Liying Peng, Ge Bai, Chunzheng Wang, Jianan Dong, Yongjun Liu, Zhe Sun, Yuguo Zhen, Guixin Qin, Xuefeng Zhang, Natnael Demelash and Tao Wang
Animals 2022, 12(13), 1718; https://doi.org/10.3390/ani12131718 - 02 Jul 2022
Cited by 2 | Viewed by 1859
Abstract
The objective of the study was to elucidate the stearoyl-coenzyme A desaturase (SCD1)-dependent gene network of c9, t11-CLA biosynthesis in MAC-T cells from an energy metabolism perspective. The cells were divided into the CAY group (firstly incubated with CAY10566, a chemical inhibitor of [...] Read more.
The objective of the study was to elucidate the stearoyl-coenzyme A desaturase (SCD1)-dependent gene network of c9, t11-CLA biosynthesis in MAC-T cells from an energy metabolism perspective. The cells were divided into the CAY group (firstly incubated with CAY10566, a chemical inhibitor of SCD1, then incubated with trans-11-octadecenoic acid, (TVA)), the TVA group (only TVA), and the control group (without CAY, TVA). The c9, t11-CLA, and TVA contents were determined by gas chromatography. The mRNA levels of SCD1 and candidate genes were analyzed via real-time PCR. Tandem mass tag (TMT)-based quantitative proteomics, bioinformatic analysis, parallel reaction monitoring (PRM), and small RNA interference were used to explore genes involved in the SCD1-dependent c9, t11-CLA biosynthesis. The results showed that the SCD1 deficiency led by CAY10566 blocked the biosynthesis of c9, t11-CLA. In total, 60 SCD1-related proteins mainly involved in energy metabolism pathways were primarily screened by TMT-based quantitative proteomics analysis. Moreover, 17 proteins were validated using PRM analysis. Then, 11 genes were verified to have negative relationships with SCD1 after the small RNA interference analysis. Based on the above results, we concluded that genes involved in energy metabolism pathways have an impact on the SCD1-dependent molecular mechanism of c9, t11-CLA biosynthesis. Full article
(This article belongs to the Special Issue The Mammary Gland Identifies Genes and Networks in Dairy Livestock)
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