COVID, Volume 2, Issue 10 (October 2022) – 13 articles

The COVID-19 pandemic required massive testing of potential patients in resource-constrained areas in Senegal. The first case of COVID-19 was reported on 2 March 2020 in Dakar city and on 10 March, the first cases were reported in Touba city, the second most populous city in Senegal. Following the scale of confirmed COVID-19 cases in Touba city, the Institut Pasteur de Dakar mobile laboratory truck (MLT) was deployed on March 13 to bring diagnostics to the point of need for better management of patient and outbreak control. The MLT deployed is a 6 × 6 truck equipped with an isolator for sample inactivation, a generator and batteries to ensure energy autonomy, and a molecular platform for pathogens detection. Nasal and oropharyngeal swabs were collected from suspected COVID-19 cases and sent to the MLT located at the Touba primary healthcare center. Samples were extracted and RNA amplified by real time qRT-PCR. A total of 11,693 samples were collected from 14 regions of Senegal and tested between March to August 2021. Within the samples tested, 10.6% (1240/1693) were positive for SARS-CoV-2. Furthermore, the MLT allowed the confirmation of the first cases of COVID-19 in 25 out of 79 health districts of Senegal. Thereby, the MLT deployment during the first 6 months of COVID-19 in Senegal allowed rapid processing of suspected case samples collected in Touba and other surrounding areas and, thus, significantly contributed to the outbreak response and early case management in Senegal. Full article

An important step towards COVID-19 pandemic control is adequate knowledge and adherence to mitigation measures, including vaccination. We assessed the level of COVID-19 knowledge, attitudes, and practices (KAP) among residents from an urban informal settlement in the City of Nairobi (Kibera), and a rural community in western Kenya (Asembo). A cross-sectional survey was implemented from April to May 2021 among randomly selected adult residents from a population-based infectious diseases surveillance (PBIDS) cohort in Nairobi and Siaya Counties. KAP questions were adopted from previous studies. Factors associated with the level of COVID-19 KAP, were assessed using multivariable regression methods. COVID-19 vaccine acceptance was 83.6% for the participants from Asembo and 59.8% in Kibera. The reasons cited for vaccine hesitancy in Kibera were safety concerns (34%), insufficient information available to decide (18%), and a lack of belief in the vaccine (21%), while the reasons in Asembo were safety concerns (55%), insufficient information to decide (26%) and lack of belief in the vaccine (11%). Our study findings suggest the need for continued public education to enhance COVID-19 knowledge, attitudes, and practices to ensure adherence to mitigation measures. Urban informal settlements require targeted messaging to improve vaccine awareness, acceptability, and uptake. Full article

Introduction: Studies conducted in real-life scenarios on vaccine protection against COVID-19 constitute an important global priority, but one that is currently mostly neglected in low- and middle-income countries such as Angola. Here, we analyze for the first-time vaccine protection against COVID-19 in a real-life scenario after 6 months of implementing a multi-vaccination plan in Angola. Methods: 4232 vaccinated and unvaccinated individuals with the result of a rapid antigen diagnostic test against SARS-CoV-2 performed from 27 to 28 December 2021 were included in the study. The general and sex-adjusted and age-adjusted odds ratios were evaluated by comparing the chances of vaccination between cases and controls, and their associated 95% CI, which were calculated using the Mantel–Haenszel stratification method. Vaccine efficacy was calculated using the odds ratio applying the accepted statistical vaccine efficacy formula: (1 − odds ratio) × 100. For all estimates, a p-value < 0.05 was considered statistically significant. Results: The odds of SARS-CoV-2 infection were 0.85 (95% CI 0.70–1.03)-times lower in vaccinated compared to unvaccinated individuals, with p = 0.09. The overall vaccine efficacy (VE) was 15% (95% CI −3–30). Conclusion: There was no statistically significant decrease in the chances of SARS-CoV-2 infection in vaccinated versus unvaccinated individuals. Full article

A new COVID-19 variant, Omicron, has emerged from South Africa, indicating that the pandemic will not be over anytime soon. An unimaginable amount of economic damage has resulted from the pandemic. Omicron and its economic implications are discussed in detail in this review article. It also includes statistics on occurrence, mortality, and recuperation in Pakistan and data on the country’s immunization coverage. Developing countries with unstable economies, such as Pakistan, have experienced increased economic difficulties. As a result, all developed and underdeveloped countries should strictly adhere to preventive measures and improvements in diagnostic tests and vaccine development to bring the pandemic under control. Full article

The severity of coronavirus disease 2019 is closely linked to dysregulated immune responses. The search for viral proteins associated with immune regulation in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical to reveal the pathogenicity of the virus. In this study, accessory proteins ORF7a (referred to as ORF7a-1 and ORF7a-2, respectively) from two SARS-related coronaviruses, severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2, were produced through the denaturing and refolding of inclusion body proteins. The recombinant protein was incubated with alveolar epithelial cells, and the transcription and expression levels of major cytokines were determined by reverse transcription-quantitative PCR and enzyme-linked immunosorbent assay. SARS-related coronavirus ORF7a can up-regulate the transcription and expression of interleukin-6, C-C motif chemokine ligand 8, interferon α, and interferon β. The results also indicated that the two highly conserved ORF7a had certain differences in promoting the transcription and expression of cytokines. The study showed that ORF7a is a virus-encoded immune regulator by alveolar epithelial cells that plays an important role in the pathogenicity of SARS-related coronaviruses. Full article

In this study, we describe how to keep a campus safe and “open” by implementing a proactive, as opposed to reactive, strategy (the Green Zone strategy). The pillars are leadership, clear communication, clean air, vaccination campaigns, and intense efforts in mass testing. Over a period of 12 months, about 277,000 pooled real-time polymerase chain reaction (RT-PCR) samples and lateral flow tests (LFTs) were collected, and 201 people were identified as COVID-19-positive. For the PCRs, we use the Lollipop technique, combined with nose swabs and gargle samples, to minimize sample-collection efforts. Importantly, not only staff, students, and contractors, but also their family members, friends, and partners; daycare centers; and local sports and arts teams, etc., were invited and participated. This outreach made it possible to propagate the tests more widely and monitor a larger network. At times of larger social gatherings—most prominently, on 23 December 2021 before Christmas (during the rise of the Omicron wave)—testing capacities were increased. The results not only demonstrate the great power of mass testing in providing an open-but-safe work environment, even if the surroundings are highly infectious (red zone), but also the strength and resilience of a university. It shows how the unique pillars of science, infrastructure, students, and independency make it possible to maneuver a community, even through unpredictable times. Full article

The United States struggled exceptionally during the COVID-19 pandemic. For researchers and policymakers, it is of great interest to understand the risk factors associated with COVID-19 when examining data aggregated at a regional level. We examined the county-level association between the reported COVID-19 case fatality rate (CFR) and various demographic, socioeconomic and health factors in two hard-hit US states: New York and Florida. In particular, we examined the changes over time in the association patterns. For each state, we divided the data into three seasonal phases based on observed waves of the COVID-19 outbreak. For each phase, we used tests of correlations to explore the marginal association between each potential covariate and the reported CFR. We used graphical models to further clarify direct or indirect associations in a multivariate setting. We found that during the early phase of the pandemic, the association patterns were complex: the reported CFRs were high, with great variation among counties. As pandemics progressed, especially during the winter phase, socioeconomic factors such as median household income and health-related factors such as the prevalence of adult smokers and mortality rate of respiratory diseases became more significantly associated with the CFR. It is remarkable that common risk factors were identified for both states. Full article

The rationale for this review paper is to take stock of the current knowledge in the literature on the intersection of telework and work–life balance—an area that has grown in importance due to the impact of the COVID-19 pandemic. The review also considers the context of the government’s role in pursuing policies to reduce the impacts of the pandemic in order to improve societal if not personal resilience, as these policies sometimes had unintended adverse impacts. After a section on the literature search method, sections follow on the literature considering telework/working from home, stress, and gender; work–life balance figures prominently in the papers reviewed. An additional category for the government and its role in concerns related to this topic follows. For future research, the differences between groups in responding to the demands of telework and work–life balance, particularly in regard to gender, are worth further investigation, as the COVID-19 pandemic has offered great challenges but also immense opportunities to learn and prepare organizations for future crises. Full article

The spread of the new coronavirus SARS-CoV-2 has substantial social, health, and economic impacts. High viral load in the air in hospitals poses a risk to medical personnel. Cold atmospheric plasma (CAP) is a new technology based on the emission in the air of reactive species, neutral particles, UV radiation, and electromagnetic field. CAP has the potential as an antiviral agent. In this study, an 80-day clinical trial took place at Nicosia General Hospital to evaluate the application of CAP devices for lowering the viral load in the COVID rooms. A total of 284 indoor environment samples were tested by RT-PCR, for which 9 were positive (~3% Positive Rate). After analyzing the initial results, an ion emitter was paired with each patient, and the results showed that the method could eliminate the virus from the COVID wards up to 100%. The number of patients discharged from the hospital in the ionizer group was 4.8% higher than in the non-ionizer group, and 45% fewer patients in the ionizer group who remained in the rooms required oxygen support. The clinical trial shows evidence that composite CAP can decrease coronavirus spread in hospital environments and potentially prevent virus transmission. Full article

COVID-19 and long COVID-19 vulnerabilities may be caused indirectly by albumin binding deficiency (ABD), which can be corrected by the correct administration of human serum albumin (HSA). The liver is the primary site of nutrient regulation and fluid volume maintenance; control of both is by changes to albumin concentration. In healthy subjects, the HSA lymphatic nutrient pump (HSALNP) ensures continual pumping of nutrients from the liver and that nutrients are appropriately distributed to organs. Nutrients are delivered to cells according to the availability of binding to HSA. The HSALNP, therefore, maintains the correct nutrient and colloidal pressure balance in all tissues independently. In unhealthy tissues, following COVID-19 infection, the passage of HSA/nutrients through the interstitial spaces and lymph will be impeded. Fluid therapy into the periphery leads to the dilution of essential nutrients attached to the protein carriers such as albumin. The levels of albumin being charged by the liver with nutrients is critical in maintaining immune stability by maintaining nutrient support and colloidal pressure of the cellular structures. The site of HSA binding by the liver is of great importance, and direct infusion of albumin into the hepatic portal vein is the most appropriate method of maintaining colloid pressure and cellular nutrient levels. Full article

Health systems challenges that emerged during the COVID-19 pandemic, such as a lack of resources and medical staff, are forcing solutions which optimize healthcare performance. One of the solutions is the development of clinical decision support systems (CDSS) based on artificial intelligence (AI). We classified AI-based clinical decision-supporting systems used during the pandemic and evaluated the mathematical algorithms present in these systems. Materials and methods: we searched for articles relevant to the aim of the study in the Scopus publication database. Results: depending on the purpose of the development a clinical decision support system based on artificial intelligence during pandemic, we identified three groups of tasks: organizational, scientific and diagnostic. Tasks such as predicting of pandemic parameters, searching of analogies in pandemic progression, prioritization of patients, use of telemedicine are solved for the purposes of healthcare organization. Artificial intelligence in drugs and vaccine development, alongside personalized treatment programs, apply to new scientific knowledge acquisition. Diagnostic tasks include the development of mathematical models for assessing COVID-19 outcomes, prediction of disease severity, analysis of factors influencing COVID-19 complications. Conclusion: artificial intelligence methods can be effectively implemented for decision support systems in solving tasks that face healthcare during pandemic. Full article

The purpose of this study was to examine the subjective reasons for hesitancy to receive COVID-19 vaccination and the sociodemographic factors associated with vaccination uptake. An online social media survey was conducted among the general Nigerian population using a self-developed questionnaire. Data were analyzed using binary logistic regression with crude and adjusted odds ratios (AOR) at a 95% confidence interval (CI) and a p value of less than 0.05. A total of 576 participants with a mean age of 31.86 years participated in the study. 28% (n = 158) received one or more doses of the COVID-19 vaccine. Teachers were significantly less likely than health professionals to be vaccinated (AOR = 0.33, 95% CI 0.16–0.69). In addition, unemployed people (AOR = 0.37, 95% CI 0.15–0.89) were less likely to be vaccinated than government employees, and those of intermediate socioeconomic status (AOR = 0.47 95% CI 0.26–0.88) were less likely to be vaccinated than were those of high socioeconomic status. Five main themes emerged regarding participants’ subjective reasons for hesitating to receive the COVID-19 vaccine: fear related to vaccine content (e.g., efficacy), negative effects on the body (e.g., blood clots), distrust of the system/government (e.g., politics), psychological concerns (e.g., anxiety), and misconceptions. Sociodemographic variables and vaccine misconceptions were found to play an important role in COVID-19 vaccination coverage in Nigeria. Full article

Fear-responses to bat-originating coronavirus pandemics with respect to quarantine imposition are gathered and interpreted from large datasets, identified and disseminated by media. Responses are effectively gauged using postmodern thought with a continuum ranging from people’s resilience to define their own perspectives to public views being socially conditioned from media persistence in maintaining fear. Public responses to the 2003 SARS pandemic generally presumed and supported resilience of citizens’ perspectives. In contrast, from late 2019 to mid-2022, public responses to the COVID-19 pandemic were media-determined, promoting fear. In this regard, reactions to the COVID-19 quarantines are contrasted to the hospital isolations of SARS. The primary source of the difference was the major polarizing influence by social media of the WHO policy makers’ pronouncements and of healthcare providers’ statements directing media spotlight in their guidance of public response to COVID-19 throughout the pandemic, unlike during SARS. An investigation of cognitive bias regarding the psychological and societal implications related to this migration from resilience to fear regarding public responses to novel bat-originating coronavirus pandemics elicits recommendations concerning future quarantine dictates. These recommendations are dependent on appropriate encouragement of hopeful resilience through evidence based practice with respect to one extreme of the postmodern thought continuum. Full article