Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolates and Data Collection
2.2. Phenotypic Characterization
2.3. Genotypic Characterization
2.4. Antifungal Susceptibility Test
3. Results
3.1. Geographic Distribution, Demographic Characteristics, and Clinical Data
3.2. Phenotypic Characteristics of S. schenckii Thai Isolates
3.3. Phylogenetic Analysis of S. schenckii Thai Isolates
3.4. Antifungal Susceptibility
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Strain | Source | Origin | Accession Number | ||
---|---|---|---|---|---|---|
ITS | CAL | b-TUB | ||||
S. schenckii | KU20017.9 | Cat | Thailand | MW819850 | MZ215901 | MZ215921 |
S. schenckii | KU2001810 | Cat | Thailand | MW819852 | MZ215902 | MZ215922 |
S. schenckii | KU20019.3 | Cat | Thailand | MW819851 | MZ215903 | MZ215923 |
S. schenckii | KU20020.45 | Cat | Thailand | MW857103 | MZ215906 | MZ215926 |
S. schenckii | KU20020.376 | Cat | Thailand | MW857105 | MZ215907 | MZ215927 |
S. schenckii | KU20020.377 | Cat | Thailand | MW857106 | MZ215908 | MZ215928 |
S. schenckii | KU20020.378 | Cat | Thailand | MW857108 | MZ215909 | MZ215929 |
S. schenckii | KU20020.379 | Cat | Thailand | MW857109 | MZ215910 | MZ215930 |
S. schenckii | KU20020.380 | Cat | Thailand | MW857111 | MZ215911 | MZ215931 |
S. schenckii | KU20020.381 | Cat | Thailand | MW857112 | MZ215912 | MZ215932 |
S. schenckii | KU20020.382 | Cat | Thailand | MW857113 | MZ215913 | MZ215933 |
S. schenckii | KU20020.383 | Human | Thailand | MW857114 | MZ215914 | MZ215934 |
S. schenckii | KU20020.384 | Cat | Thailand | MW857115 | MZ215915 | MZ215935 |
S. schenckii | KU20020.385 | Cat | Thailand | MW857126 | MZ215916 | MZ215936 |
S. schenckii | KU20020.386 | Cat | Thailand | MW857128 | MZ215917 | MZ215937 |
S. schenckii | KU20021.02 | Cat | Thailand | MW857129 | MZ215919 | MZ215939 |
S. schenckii | KU20021.05 | Cat | Thailand | MZ882155 | OK065908 | OK065918 |
S. schenckii | KU20021.06 | Cat | Thailand | MZ882257 | OK065909 | OK065919 |
S. schenckii | KU20021.07 | Cat | Thailand | MZ882288 | OK065910 | OK065920 |
S. schenckii | KU20021.08 | Cat | Thailand | MZ882289 | OK065911 | OK065921 |
S. schenckii | KU20021.10 | Cat | Thailand | MZ882290 | OK065912 | OK065922 |
S. schenckii | KU20021.11 | Cat | Thailand | MZ882291 | OK065913 | OK065923 |
S. schenckii | KU20021.12 | Cat | Thailand | MZ882292 | OK065914 | OK065924 |
S. schenckii | KU20021.13 | Cat | Thailand | MZ882293 | OK065915 | OK065925 |
S. schenckii | KU20021.40 | Cat | Thailand | MZ882294 | OK065916 | OK065926 |
S. schenckii | KU20021.42 | Cat | Thailand | MZ882295 | OK065917 | OK065927 |
S. schenckii | UTHSC 04-1064 | Human | USA | - | AM399014 | AM747285 |
S. schenckii | UTHSC 04-771 | Human | USA | - | AM398985 | AM747287 |
S. schenckii | UdeA_0001 | Human | Columbia | - | ON060845 | MG953922 |
S. schenckii | UdeA_0007 | ND | Columbia | - | ON060848 | ON023043 |
S. schenckii | UdeA_0008 | ND | Columbia | - | ON060849 | ON023044 |
S. schenckii | UdeA_22 | ND | Columbia | - | ON060860 | MG953936 |
S. schenckii | UdeA_220 | Human | Columbia | - | ON060861 | MG953935 |
S. schenckii | UdeA 1023 | Human | Columbia | - | ON060850 | MG953933 |
S. schenckii | UdeA 1027 | ND | Columbia | - | ON060851 | MG953934 |
S. schenckii | UdeA_3017 | Human | Columbia | - | ON060863 | MG953916 |
S. schenckii | UdeA_4015 | ND | Columbia | - | ON060864 | ON023050 |
S. schenckii | UdeA_4023 | ND | Columbia | - | ON060865 | MG953917 |
S. schenckii | UdeA_4047 | ND | Columbia | - | ON060866 | ON023051 |
S. schenckii | UdeA_6450 | Human | Columbia | - | ON060870 | MG953918 |
S. schenckii | UdeA_6514 | ND | Columbia | - | ON060871 | ON023052 |
S. schenckii | UdeA_7011 | ND | Columbia | - | ON060872 | ON023053 |
S. schenckii | UdeA_7027 | Human | Columbia | - | ON060873 | MH174665 |
S. schenckii | UdeA_8004 | Human | Columbia | - | ON060874 | MG953919 |
S. schenckii | UdeA_9043 | Human | Columbia | - | ON060876 | MH174668 |
S. schenckii | UdeA 10937 | Human | Columbia | - | ON060852 | MG953927 |
S. schenckii | UdeA 11024 | Human | Columbia | - | ON060853 | MG953925 |
S. schenckii | UdeA 15565 | Human | Columbia | - | ON060857 | MG953924 |
S. schenckii | UdeA 15741 | Human | Columbia | - | ON060858 | MG953920 |
S. schenckii | UdeA 20008 | Human | Columbia | - | ON060859 | MG953921 |
S. schenckii | PSUV_SS18-1 | Cat | Thailand | - | ON211523 | ON211485 |
S. schenckii | PSUV_SS18-2 | Cat | Thailand | - | ON211527 | ON211489 |
S. schenckii | PSUV_SS18-3 | Cat | Thailand | - | ON211510 | ON211472 |
S. schenckii | PSUV_SS18-4 | Cat | Thailand | - | ON211508 | ON211470 |
S. schenckii | PSUV_SS18-6 | Cat | Thailand | - | ON211517 | ON211479 |
S. schenckii | PSUV_SS18-7 | Cat | Thailand | - | ON211511 | ON211473 |
S. schenckii | PSUV_SS18-8 | Cat | Thailand | - | ON211520 | ON211482 |
S. schenckii | PSUV_SS18-9 | Cat | Thailand | - | ON211519 | ON211481 |
S. schenckii | PSUV_SS18-10 | Cat | Thailand | - | ON211529 | ON211491 |
S. schenckii | PSUV_SS19-1 | Cat | Thailand | - | ON211515 | ON211477 |
S. schenckii | PSUV_SS19-2 | Cat | Thailand | - | ON211513 | ON211475 |
S. schenckii | PSUV_SS19-3 | Cat | Thailand | - | ON211514 | ON211476 |
S. schenckii | PSUV_SS19-5 | Cat | Thailand | - | ON211542 | ON211504 |
S. schenckii | PSUV_SS19-6 | Cat | Thailand | - | ON211530 | ON211492 |
S. schenckii | PSUV_SS19-7 | Cat | Thailand | - | ON211540 | ON211502 |
S. schenckii | PSUV_SS19-8 | Cat | Thailand | - | ON211524 | ON211486 |
S. schenckii | PSUV_SS19-9 | Cat | Thailand | - | ON211518 | ON211480 |
S. schenckii | PSUV_SS19-10 | Cat | Thailand | - | ON211529 | ON211491 |
S. schenckii | PSUV_SS20-3 | Cat | Thailand | - | ON211516 | ON211478 |
S. schenckii | PSUV_SS20-4 | Cat | Thailand | - | ON211543 | ON211505 |
S. schenckii | PSUV_SS20-5 | Cat | Thailand | - | ON211525 | ON211487 |
S. schenckii | PSUV_SS20-6 | Cat | Thailand | - | ON211534 | ON211496 |
S. schenckii | PSUV_SS20-7 | Cat | Thailand | - | ON211539 | ON211501 |
S. schenckii | PSUV_SS20-8 | Cat | Thailand | - | ON211521 | ON211483 |
S. schenckii | PSUV_SS20-9 | Cat | Thailand | - | ON211522 | ON211484 |
S. schenckii | PSUV_SS21-1 | Cat | Thailand | - | ON211535 | ON211497 |
S. schenckii | PSUV_SS21-2 | Cat | Thailand | - | ON211537 | ON211499 |
S. schenckii | PSUV_SS21-3 | Cat | Thailand | - | ON211538 | ON211500 |
S. schenckii | PSUV_SS21-5 | Cat | Thailand | - | ON211544 | ON211506 |
S. schenckii | PSUV_SS21-6 | Cat | Thailand | - | ON211545 | ON211507 |
S. schenckii | PSUV_SS20-10 | Cat | Thailand | - | ON211541 | ON211503 |
S. schenckii | PSUV_SS20-11 | Cat | Thailand | - | ON211526 | ON211488 |
S. schenckii | PSUV_SS20-12 | Cat | Thailand | - | ON211528 | ON211490 |
S. schenckii | PSUV_SS20-13 | Cat | Thailand | - | ON211512 | ON211474 |
S. schenckii | PSUV_SS20-14 | Cat | Thailand | - | ON211531 | ON211493 |
S. schenckii | PSUV_SS20-15 | Cat | Thailand | - | ON211533 | ON211495 |
S. schenckii | PSUV_SS20-16 | Cat | Thailand | - | ON211532 | ON211494 |
S. schenckii | PSUV_SS20-17 | Cat | Thailand | - | ON211536 | ON211498 |
S. schenckii | NBRC_8158 | ND | Japan | - | AM117438 | AM116910 |
S. schenckii | IHEM_3774 | Human | Colombia | - | AM117447 | AM116921 |
S. schenckii | IHEM_3787 | ND | South Africa | - | AM117435 | AM116925 |
S. schenckii | IHEM_15502 | Human | Peru | - | AM117427 | AM116927 |
S. schenckii | IHEM_15503 | Human | Peru | - | AM117433 | AM116930 |
S. schenckii | IHEM_15508 | Human | Peru | - | AM117443 | AM116924 |
S. schenckii | IHEM_15511 | Human | Peru | - | AM117440 | AM116917 |
S. schenckii | IHEM_15477 | Human | Bolivia | - | AM117444 | AM116916 |
S. schenckii | IHEM_15486 | Human | Peru | - | AM117432 | AM116929 |
S. schenckii | IHEM_15489 | Human | Peru | - | AM117430 | AM116926 |
S. schenckii | IHEM_15499 | Human | Peru | - | AM117434 | AM116928 |
S. schenckii | FMR_8604 | Human | Peru | - | AM117429 | AM116914 |
S. schenckii | FMR_8605 | Human | Peru | - | AM117442 | AM116923 |
S. schenckii | FMR_8606 | Human | Peru | - | AM117431 | AM116913 |
S. schenckii | FMR_8608 | Human | Peru | - | AM117441 | AM116919 |
S. schenckii | FMR_8609 | Human | Peru | - | AM117439 | AM116918 |
S. schenckii | FMR_8677 | Human | Argentina | - | AM117436 | AM116915 |
S. schenckii | FMR_8678 | Human | Argentina | - | AM117446 | AM116920 |
S. schenckii | FMR_8679 | Human | Argentina | - | AM117445 | AM116922 |
S. schenckii | EH-195 | ENV | Mexico | - | ON060840 | ON023042 |
S. schenckii | EH-197 | Human | Mexico | - | ON060841 | MH174664 |
S. schenckii | EH-230 | Human | Guatemala | - | ON060842 | MG953930 |
S. schenckii | EH-251 | ENV | Mexico | - | ON060843 | MG953938 |
S. schenckii | EH-254 | ENV | Mexico | - | ON060844 | MG953937 |
S. schenckii | 1099-18 | Human | USA | - | JF313360 | AXCR01000005 |
S. schenckii | VSMU-SS1702 | Human | Thailand | MG270182 | - | - |
S. schenckii | NUBS14005 | Cat | Malaysia | LC012513 | - | - |
S. schenckii | NUBS14007 | Cat | Malaysia | LC012518 | - | - |
S. schenckii | NUBS14009 | Cat | Malaysia | LC012520 | - | - |
S. schenckii | NUBS14013 | Cat | Malaysia | LC012599 | - | - |
S. schenckii | NUBS14016 | Cat | Malaysia | LC012602 | - | - |
S. schenckii | NUBS14018 | Cat | Malaysia | LC012604 | - | - |
S. schenckii | NUBS14019 | Cat | Malaysia | LC012605 | - | - |
S. schenckii | FMR 8609 | Human | Peru | - | AM117439 | AM116918 |
S. schenckii | CBS 359.36T | Human | USA | KP017100 | AM117437 | AM116911 |
S. schenckii | CBS 444.67 | ND | Mozambique | KP017099 | - | - |
S. schenckii | CBS 130097 | Human | Bolivia | KC113218 | - | - |
S. schenckii | CBS 130098 | Human | Peru | KP017091 | - | - |
S. schenckii | ATCC 10268 | Human | USA | AB122038 | - | - |
S. schenckii | CBS 130111 | Human | Colombia | KC113219 | - | - |
S. schenckii | CBS 130103 | Human | Argentina | KC113222 | - | - |
S. schenckii | CBS 130114 | Human | Peru | KC113221 | - | - |
S. schenckii | CBS 130101 | Human | Peru | KP017095 | - | - |
S. schenckii | CBS 130112 | Human | Peru | KC113223 | - | - |
S. schenckii | CBS 211.61 | ND | South Africa | KP017093 | - | - |
S. schenckii | CBS 938.72 | Human | France | KP017094 | - | - |
S. schenckii | CBS 130100 | Human | Argentina | KC113217 | - | - |
S. globosa | CBS 130117 | Human | Japan | KC113229 | - | - |
S. globosa | CBS 120340 T | Human | Spain | KC113229 | KP101459. | WOEH00000000 |
S. globosa | CBS 129720 | ND | China | - | KP101463 | KC113237 |
S. globosa | CBS 129722 | Human | China | - | KP101464 | KC113236 |
S. globosa | CBS 129724 | Human | China | - | KP101466 | KC113238 |
S. globosa | UdeA 0003 | ND | Columbia | - | ON060846 | MG953948 |
S. globosa | UdeA 0004 | ND | Columbia | - | ON060847 | MG953945 |
S. globosa | UdeA 64 | ND | Columbia | - | ON060869 | MG953946 |
S. globosa | UdeA 5048 | ND | Columbia | - | ON060867 | MG953939 |
S. globosa | UdeA 6004 | ND | Columbia | - | ON060868 | MG953940 |
S. globosa | UdeA 8029 | ND | Columbia | - | ON060875 | MH174666 |
S. globosa | UdeA 9051 | ND | Columbia | - | ON060877 | MG953941 |
S. globosa | UdeA 12926 | ND | Columbia | - | ON060855 | MH174667 |
S. globosa | NCCPF 220126 | Human | India | - | MN243809 | KX881724 |
S. globosa | NCCPF 220127 | Human | India | - | MN243810 | KX881725 |
S. globosa | NCCPF 220129 | Human | India | - | MN243811 | KX881701 |
S. globosa | NCCPF 220135 | Human | India | - | MN243812 | KX881721 |
S. globosa | NCCPF 220136 | Human | India | - | MN243813 | KX881722 |
S. globosa | NCCPF 220146 | Human | India | - | MN243822 | KX881719 |
S. globosa | NCCPF 220149 | Human | India | - | MN243823 | MN257636 |
S. luriei | CBS 937.72 T | Human | South Africa | AB128012 | - | - |
S. brasiliensis | CBS 120339 T | Human | Brazil | KP017087 | - | - |
S. brasiliensis | CBS 130106 | Human | Brazil | KC113212 | - | - |
S. brasiliensis | CBS 130109 | Human | Brazil | KC113213 | - | - |
S. brasiliensis | CF2018 | ND | Argentina | - | MK850446 | MK850450 |
S. brasiliensis | CHS2018 | ND | Argentina | - | MK850448 | MK850452 |
S. brasiliensis | CN2018 | ND | Argentina | - | MK850447 | MK850451 |
S. brasiliensis | IPEC_16919 | Human | Brazil | - | AM116898 | AM116934 |
S. pallida | CBS 150.87 T | ENV | Germany | EF127879 | - | - |
S. chilensis | CBS 139891 T | Human | Chile | KP711811 | - | - |
Characteristic | Number (%) | |
---|---|---|
Case number | 38 | |
Geographic origin | Bangkok | 32 (85) |
Nonthaburi | 2 (5) | |
Pathum Thani | 2 (5) | |
Ayutthaya | 2 (5) | |
Age (years) | mean (±standard deviation) | 3.96 (±3.93) |
Youngest | 6 M | |
Oldest | 14 Y 10 M | |
Sex | Male | 23 (61) |
Female | 15 (39) | |
Breed | Domestic short hair | 36 (95) |
Purebred | 2 (5) | |
Castration | Yes | 22 (58) |
No | 16 (42) | |
Cat rearing | Indoor only | 9 (24) |
Outdoor access | 29 (76) | |
Possible transmission | Fights with other cats | 19 (50) |
None | 11 (29) | |
Unknown | 8 (21) | |
FeLV/FIV infected | Yes | 2 (5) |
No | 21 (55) | |
No data | 15 (40) | |
Number of lesions | 1 | 9 (24) |
2 | 11 (29) | |
≥3 | 18 (47) | |
The form of lesion | Ulcer | 35 (92) |
Granulomatous nodular | 3 (8) | |
Other organ involvement | Respiratory system | 8 (21) |
Eyes lids | 2 (5) | |
Bone | 1 (3) | |
None | 27 (71) | |
Therapeutics | Antifungal drugs | 31 (82) |
Combined surgy and anti-fungal drugs | 7 (18) | |
Outcome | Recovery | 20 (53) |
Stable disease and continue treatment | 4 (10) | |
death | 14 (37) |
Growth Condition | Growth (Number, %) and Average Colony Diameter (mm) | |||
---|---|---|---|---|
No Growth | Mold | Yeast-Like | Yeast | |
30 °C | ||||
SDA | 0 | 5, 19 (6.99) | 21, 81 (3.41) | 0 |
PDA | 0 | 5, 19 (9.34) | 21, 81 (4.11) | 0 |
BHIA | 0 | 0 | 13, 50 (4.11) | 13, 50 (4.15) |
35 °C | ||||
SDA | 0 | 1, 4 (0.74) | 22, 85 (1.74) | 3, 12 (2.24) |
PDA | 0 | 1, 4 (1.77) | 19, 73 (1.97) | 6, 23 (2.15) |
BHIA | 0 | 0 | 4, 15 (3.91) | 22, 85 (3.33) |
37 °C | ||||
SDA | 8 (31) | 0 | 6, 23 (0.39) | 12, 46 (0.48) |
PDA | 0 | 8, 31 (0.18) | 10, 38 (0.38) | |
BHIA | 0 | 3, 11 (0.46) | 15, 58 (0.98) |
Agents | Yeast Phase | Mold Phase | ||||||
---|---|---|---|---|---|---|---|---|
MIC Range | Mode | MIC50 | MIC90 | MIC Range | Mode | MIC50 | MIC90 | |
AMB | 0.25 to 4 | 2 | 1 | 4 | 0.25 to 4 | 0.5 | 0.5 | 4 |
ITR | 0.25 to >32 | 1 | 1 | 4 | 0.0625 to ≥32 | 0.5 | 0.5 | 4 |
POS | 0.25 to >32 | 0.5 | 0.5 | 8 | 0.0625 to ≥32 | 0.25 | 0.25 | 8 |
TERB | 0.0156 to 2 | 1 | 0.5 | 2 | 0.0312 to 4 | 1 | 0.75 | 2 |
KET | 0.0039 to 1 | 0.5 | 0.25 | 0.5 | 0.0039 to 1 | 0.25 | 0.25 | 0.5 |
MCZ | 0.0039 to 1 | 0.5 | 0.25 | 0.5 | 0.0039 to 1 | 0.25 | 0.25 | 0.5 |
FLU | 32 to >256 | >256 | >256 | >256 | 32 to >256 | >256 | >256 | >256 |
GRI | 0.0625 to ≥32 | 4 | 4 | 32 | 0.0625 to ≥32 | 4 | 4 | 16 |
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Yingchanakiat, K.; Limsivilai, O.; Sunpongsri, S.; Niyomtham, W.; Lugsomya, K.; Yurayart, C. Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand. J. Fungi 2023, 9, 590. https://doi.org/10.3390/jof9050590
Yingchanakiat K, Limsivilai O, Sunpongsri S, Niyomtham W, Lugsomya K, Yurayart C. Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand. Journal of Fungi. 2023; 9(5):590. https://doi.org/10.3390/jof9050590
Chicago/Turabian StyleYingchanakiat, Kanokporn, Orawan Limsivilai, Supita Sunpongsri, Waree Niyomtham, Kittitat Lugsomya, and Chompoonek Yurayart. 2023. "Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand" Journal of Fungi 9, no. 5: 590. https://doi.org/10.3390/jof9050590