Cisd2 Preserves the Youthful Pattern of the Liver Proteome during Natural Aging of Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Liver Histopathology
2.3. Immunohistochemistry
2.4. Western Blotting
2.5. Liver Protein Extraction and Proteomics Analysis
2.6. Statistical Analysis
3. Results
3.1. Cisd2 Attenuates Age-Related Fat Accumulation and Improves Histopathology in the Liver
3.2. The Youthful Pattern of the Liver Proteome Is Preserved in Old Cisd2 Transgenic Mice
3.3. Cisd2 Ameliorates Age-Related Dysregulation of Lipid Metabolism
3.4. Cisd2 Modulates the Redox Status, Calcium Homeostasis and Mitochondrial Respiration
3.5. Cisd2 Maintains Intracellular Protein Homeostasis
4. Discussion
4.1. De Novo Lipogenesis and Lipid Droplet Homeostasis
4.2. The Compensatory Role of β-Oxidation in Aging-Induced NAFLD
4.3. Cisd2 Regulates Oxidative Stress and the Antioxidant Response
4.4. Cisd2 Preserves Mitochondrial ETC and Ribosomal Protein Complexes
4.5. IRS/AKT Signaling during Liver Aging
4.6. Activation of the Proteasome-Dependent Degradation Pathway during Aging
4.7. CISD2 Is a Promising Target for Developing Novel Therapies to Treat NAFLD
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huang, C.-H.; Huang, Y.-L.; Shen, Z.-Q.; Lin, C.-H.; Tsai, T.-F. Cisd2 Preserves the Youthful Pattern of the Liver Proteome during Natural Aging of Mice. Biomedicines 2021, 9, 1229. https://doi.org/10.3390/biomedicines9091229
Huang C-H, Huang Y-L, Shen Z-Q, Lin C-H, Tsai T-F. Cisd2 Preserves the Youthful Pattern of the Liver Proteome during Natural Aging of Mice. Biomedicines. 2021; 9(9):1229. https://doi.org/10.3390/biomedicines9091229
Chicago/Turabian StyleHuang, Chen-Hua, Yi-Long Huang, Zhao-Qing Shen, Chao-Hsiung Lin, and Ting-Fen Tsai. 2021. "Cisd2 Preserves the Youthful Pattern of the Liver Proteome during Natural Aging of Mice" Biomedicines 9, no. 9: 1229. https://doi.org/10.3390/biomedicines9091229