Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Adipose-Derived Stem Cells Isolation and Expansion
2.3. Adipose-Derived Stem Cells Characterization
2.4. Adipose-Derived Stem Cells Transduction
2.5. Surgical Procedure
2.6. Cell Transplantation
2.7. In Vivo Cell Tracking
2.8. Euthanasia and Tissue Collection
2.9. Anatomopathological Study—Histology and Immunohistochemistry
2.10. Statistical Analysis
3. Results
3.1. Animal Care and Surgical Procedures
3.2. ADSC Characterization
3.3. Histomophometric Analysis of Myelin and Neurons
3.4. Immunohistochemical Astrocyte Evaluation
3.5. In Vivo Cell Tracking with Bioluminescence Imaging
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Area | Group | N | Average | Median | Min | Max | SD |
---|---|---|---|---|---|---|---|
AR | CG1 | 9 | 19,738 | 18,760 | 8690 | 28,785 | 6500 |
CG2 | 10 | 16,778 | 15,287 | 7870 | 36,177 | 8326 | |
EG1 | 8 | 21,547 | 21,702 | 15,805 | 26,511 | 3597 | |
EG2 | 9 | 20,042 | 17,650 | 10,312 | 34,553 | 8369 | |
AL | CG1 | 9 | 19,882 | 21,492 | 7922 | 27,080 | 6766 |
CG2 | 10 | 13,586 | 12,688 | 5525 | 25,587 | 6325 | |
EG1 | 8 | 21,256 | 20,114 | 15,863 | 29,074 | 4339 | |
EG2 | 9 | 18,629 | 18,453 | 9779 | 37,710 | 9531 | |
PR | CG1 | 9 | 20,139 | 20,121 | 8302 | 30,488 | 6559 |
CG2 | 10 | 18,034 | 16,628 | 8737 | 32,206 | 7045 | |
EG1 | 8 | 24,795 | 24,927 | 15,770 | 29,270 | 4107 | |
EG2 | 9 | 19,064 | 21,752 | 10,207 | 31,100 | 7589 | |
PL | CG1 | 9 | 21,411 | 21,664 | 10,541 | 29,412 | 5929 |
CG2 | 10 | 15,765 | 16,124 | 8161 | 22,956 | 4404 | |
EG1 | 8 | 23,672 | 23,148 | 16,040 | 31,780 | 5776 | |
EG2 | 9 | 19,058 | 13,830 | 11,131 | 38,737 | 10,120 |
p * Value | ||||
---|---|---|---|---|
Groups | AR | AL | PR | PL |
EG1 × EG2 | 0.633 | 0.486 | 0.077 | 0.275 |
CG1 × CG2 | 0.404 | 0.051 | 0.511 | 0.033 |
EG1 × CG1 | 0.497 | 0.631 | 0.105 | 0.439 |
EG2 × CG2 | 0.407 | 0.188 | 0.763 | 0.387 |
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Vialle, E.N.; Fracaro, L.; Barchiki, F.; Dominguez, A.C.; Arruda, A.d.O.; Olandoski, M.; Brofman, P.R.S.; Kuniyoshi Rebelatto, C.L. Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats. Biomedicines 2023, 11, 1394. https://doi.org/10.3390/biomedicines11051394
Vialle EN, Fracaro L, Barchiki F, Dominguez AC, Arruda AdO, Olandoski M, Brofman PRS, Kuniyoshi Rebelatto CL. Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats. Biomedicines. 2023; 11(5):1394. https://doi.org/10.3390/biomedicines11051394
Chicago/Turabian StyleVialle, Emiliano Neves, Letícia Fracaro, Fabiane Barchiki, Alejandro Correa Dominguez, André de Oliveira Arruda, Marcia Olandoski, Paulo Roberto Slud Brofman, and Carmen Lúcia Kuniyoshi Rebelatto. 2023. "Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats" Biomedicines 11, no. 5: 1394. https://doi.org/10.3390/biomedicines11051394